PMID- 31676685
OWN - NLM
STAT- MEDLINE
DCOM- 20200615
LR  - 20210314
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 294
IP  - 49
DP  - 2019 Dec 6
TI  - Desumoylation of RNA polymerase III lies at the core of the Sumo stress response 
      in yeast.
PG  - 18784-18795
LID - S0021-9258(20)30428-2 [pii]
LID - 10.1074/jbc.RA119.009721 [doi]
AB  - Post-translational modification by small ubiquitin-like modifier (Sumo) regulates 
      many cellular processes, including the adaptive response to various types of 
      stress, referred to as the Sumo stress response (SSR). However, it remains 
      unclear whether the SSR involves a common set of core proteins regardless of the 
      type of stress or whether each particular type of stress induces a 
      stress-specific SSR that targets a unique, largely nonoverlapping set of Sumo 
      substrates. In this study, we used MS and a Gene Ontology approach to identify 
      differentially sumoylated proteins during heat stress, hyperosmotic stress, 
      oxidative stress, nitrogen starvation, and DNA alkylation in Saccharomyces 
      cerevisiae cells. Our results indicate that each stress triggers a specific SSR 
      signature centered on proteins involved in transcription, translation, and 
      chromatin regulation. Strikingly, whereas the various stress-specific SSRs were 
      largely nonoverlapping, all types of stress tested here resulted in desumoylation 
      of subunits of RNA polymerase III, which correlated with a decrease in tRNA 
      synthesis. We conclude that desumoylation and subsequent inhibition of RNA 
      polymerase III constitutes the core of all stress-specific SSRs in yeast.
CI  - (c) 2019 Nguea P et al.
FAU - Nguea P, Aurelie
AU  - Nguea P A
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Norwegian 
      Radium Hospital, Montebello, N-0379 Oslo, Norway; Centre for Cancer Cell 
      Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of 
      Oslo, 0371 Oslo, Norway; Department of Biosciences, Faculty of Mathematics and 
      Natural Sciences, University of Oslo, 0371 Oslo, Norway.
FAU - Robertson, Joseph
AU  - Robertson J
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Norwegian 
      Radium Hospital, Montebello, N-0379 Oslo, Norway; Centre for Cancer Cell 
      Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of 
      Oslo, 0371 Oslo, Norway.
FAU - Herrera, Maria Carmen
AU  - Herrera MC
AD  - Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, 0371 Oslo, Norway; Department of Biosciences, 
      Faculty of Mathematics and Natural Sciences, University of Oslo, 0371 Oslo, 
      Norway.
FAU - Chymkowitch, Pierre
AU  - Chymkowitch P
AD  - Department of Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, 
      Norway. Electronic address: pierre.chymkowitch@rr-research.no.
FAU - Enserink, Jorrit M
AU  - Enserink JM
AD  - Department of Molecular Cell Biology, Institute for Cancer Research, Norwegian 
      Radium Hospital, Montebello, N-0379 Oslo, Norway; Centre for Cancer Cell 
      Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of 
      Oslo, 0371 Oslo, Norway; Department of Biosciences, Faculty of Mathematics and 
      Natural Sciences, University of Oslo, 0371 Oslo, Norway. Electronic address: 
      jorrit.enserink@rr-research.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191101
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
MH  - Mass Spectrometry
MH  - Oxidative Stress
MH  - Protein Processing, Post-Translational
MH  - RNA Polymerase III/*metabolism
MH  - Saccharomyces cerevisiae/*enzymology/*metabolism
MH  - Saccharomyces cerevisiae Proteins/*metabolism
PMC - PMC6901327
OTO - NOTNLM
OT  - Nutrient starvation
OT  - RNA polymerase III
OT  - Saccharomyces cerevisiae
OT  - gene regulation
OT  - mass spectrometry (MS)
OT  - post-translational modification (PTM)
OT  - small ubiquitin-like modifier (SUMO)
OT  - stress response
OT  - transcription regulation
OT  - transfer RNA (tRNA)
COIS- The authors declare that they have no conflicts of interest with the contents of 
      this article
EDAT- 2019/11/05 06:00
MHDA- 2020/06/17 06:00
PMCR- 2020/12/06
CRDT- 2019/11/03 06:00
PHST- 2019/06/07 00:00 [received]
PHST- 2019/10/30 00:00 [revised]
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/11/03 06:00 [entrez]
PHST- 2020/12/06 00:00 [pmc-release]
AID - S0021-9258(20)30428-2 [pii]
AID - RA119.009721 [pii]
AID - 10.1074/jbc.RA119.009721 [doi]
PST - ppublish
SO  - J Biol Chem. 2019 Dec 6;294(49):18784-18795. doi: 10.1074/jbc.RA119.009721. Epub 
      2019 Nov 1.