PMID- 31680128
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20200720
IS  - 0300-0729 (Print)
IS  - 0300-0729 (Linking)
VI  - 58
IP  - 1
DP  - 2020 Feb 1
TI  - Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal 
      epithelial cells.
PG  - 66-73
LID - 10.4193/Rhin19.172 [doi]
AB  - BACKGROUND: Airway inflammation and excessive mucin production are 
      pathophysiological characteristics of airway diseases. Fipronil, a pesticide, is 
      being extensively used in agriculture and veterinary medicine worldwide. However, 
      this compound impairs immune function in non-target organisms. The present study 
      aimed to evaluate the effect of fipronil on pro-inflammatory cytokine and mucus 
      production and signalling pathways in human primary nasal METHODOLOGY: The effect 
      of fipronil on pro-inflammatory cytokine and MUC5AC expression and the signalling 
      pathway of fipronil were investigated using real-time PCR, enzyme immunoassays, 
      immunofluorescence, and immunoblot analysis with specific inhibitors and small 
      interfering RNA. RESULTS: Fipronil treatment increased pro-inflammatory cytokine 
      interleukin (IL)-1beta, IL-6, IL-8, and MUC5AC expression in human primary nasal 
      epithelial cells. It also induced phosphorylation of extracellular 
      signal-regulated kinase 1/2 (ERK1/2) mitogenactivated protein kinase (MAPK), p38 
      MAPK, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB). 
      MAPK and NF-kB inhibitor treatment significantly inhibited increases in IL-1beta, 
      IL-6, IL-8, and MUC5AC expression. Ex vivo data confirmed that fipronil-induced 
      MUC5AC expression occurs through ERK1/2, p38, and NF-kB signalling pathways in 
      nasal inferior turbinate tissue. CONCLUSIONS: Fipronil induced pro-inflammatory 
      cytokine IL-1beta, IL-6, IL-8, and MUC5AC expression via ERK1/2 MAPK, p38 MAPK, 
      and NF-kB in human primary nasal epithelial cells.
FAU - Kwak, S
AU  - Kwak S
AD  - Department of Medical Science, College of Medicine, Graduate School of Yeungnam 
      University, Daegu, Republic of Korea; Department of Otorhinolaryngology-Head and 
      Neck Surgery, College of Medicine, Yeungnam University, Daegu, Republic of Korea.
FAU - Choi, Y S
AU  - Choi YS
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, 
      Yeungnam University, Daegu, Republic of Korea.
FAU - Na, H G
AU  - Na HG
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, 
      Yeungnam University, Daegu, Republic of Korea.
FAU - Bae, C H
AU  - Bae CH
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, 
      Yeungnam University, Daegu, Republic of Korea.
FAU - Song, S-Y
AU  - Song SY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, 
      Yeungnam University, Daegu, Republic of Korea.
FAU - Kim, Y-D
AU  - Kim YD
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, 
      Yeungnam University, Daegu, Republic of Korea; Regional Center for Respiratory 
      Diseases, Yeungnam University Medical Center, Daegu, Republic of Korea.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Rhinology
JT  - Rhinology
JID - 0347242
RN  - 0 (Cytokines)
RN  - 0 (MUC5AC protein, human)
RN  - 0 (Mucin 5AC)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrazoles)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - QGH063955F (fipronil)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Mucin 5AC/*metabolism
MH  - NF-kappa B/metabolism
MH  - Pyrazoles/*pharmacology
MH  - Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2019/11/05 06:00
MHDA- 2020/07/21 06:00
CRDT- 2019/11/05 06:00
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
PHST- 2019/11/05 06:00 [entrez]
AID - 2192 [pii]
AID - 10.4193/Rhin19.172 [doi]
PST - ppublish
SO  - Rhinology. 2020 Feb 1;58(1):66-73. doi: 10.4193/Rhin19.172.