PMID- 31680444
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20210317
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 24
IP  - 1
DP  - 2020 Jan
TI  - LncRNA HOXA11-AS regulates calcium oxalate crystal-induced renal inflammation via 
      miR-124-3p/MCP-1.
PG  - 238-249
LID - 10.1111/jcmm.14706 [doi]
AB  - Long noncoding RNA (lncRNA) has been suggested to play an important role in a 
      variety of diseases over the past decade. In a previous study, we identified a 
      novel lncRNA, termed HOXA11-AS, which was significantly up-regulated in calcium 
      oxalate (CaOx) nephrolithiasis. However, the biological function of HOXA11-AS in 
      CaOx nephrolithiasis remains poorly defined. Here, we demonstrated that HOXA11-AS 
      was significantly up-regulated in CaOx nephrolithiasis both in vivo and in vitro. 
      Gain-/loss-of-function studies revealed that HOXA11-AS inhibited proliferation, 
      promoted apoptosis and aggravated cellular damage in HK-2 cells exposed to 
      calcium oxalate monohydrate (COM). Further investigations showed that HOXA11-AS 
      regulated monocyte chemotactic protein 1 (MCP-1) expression in HK-2 cell model of 
      CaOx nephrolithiasis. In addition, online bioinformatics analysis and 
      dual-luciferase reporter assay results showed that miR-124-3p directly bound to 
      HOXA11-AS and the 3'UTR of MCP-1. Furthermore, rescue experiment results revealed 
      that HOXA11-AS functioned as a competing endogenous RNA to regulate MCP-1 
      expression through sponging miR-124-3p and that overexpression of miR-124-3p 
      restored the inhibitory effect of proliferation, promotion effects of apoptosis 
      and cell damage induced by HOXA11-AS overexpression. Taken together, HOXA11-AS 
      mediated CaOx crystal-induced renal inflammation via the miR-124-3p/MCP-1 axis, 
      and this outcome may provide a good potential therapeutic target for 
      nephrolithiasis.
CI  - (c) 2019 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Li, Yinhui
AU  - Li Y
AUID- ORCID: 0000-0001-7900-6103
AD  - Department of Nephrology, Changhai Hospital, The Naval Military Medical 
      University, Shanghai, China.
FAU - Yan, Guiling
AU  - Yan G
AD  - Department of Breast and Thyroid Surgery, Changhai Hospital, The Naval Military 
      Medical University, Shanghai, China.
AD  - Department of General Surgery, The Naval Hospital, Eastern Theater PLA, Zhoushan, 
      Zhejiang, China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Department of Nephrology, Changhai Hospital, The Naval Military Medical 
      University, Shanghai, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Nephrology, Changhai Hospital, The Naval Military Medical 
      University, Shanghai, China.
FAU - Ding, Tao
AU  - Ding T
AD  - Department of Nephrology, Changhai Hospital, The Naval Military Medical 
      University, Shanghai, China.
FAU - Yin, Yupeng
AU  - Yin Y
AD  - Department of Medical Genetics, The Naval Military Medical University, Shanghai, 
      China.
FAU - Li, Minghan
AU  - Li M
AD  - Department of Medical Genetics, The Naval Military Medical University, Shanghai, 
      China.
FAU - Zhu, Yiqing
AU  - Zhu Y
AD  - Department of Medical Genetics, The Naval Military Medical University, Shanghai, 
      China.
FAU - Sun, Shuhan
AU  - Sun S
AD  - Department of Medical Genetics, The Naval Military Medical University, Shanghai, 
      China.
FAU - Yuan, Ji Hang
AU  - Yuan JH
AD  - Department of Medical Genetics, The Naval Military Medical University, Shanghai, 
      China.
FAU - Guo, Zhiyong
AU  - Guo Z
AUID- ORCID: 0000-0001-7816-3270
AD  - Department of Nephrology, Changhai Hospital, The Naval Military Medical 
      University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191103
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Chemokine CCL2)
RN  - 0 (MIRN124 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - Nephrolithiasis, Calcium Oxalate
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Apoptosis/drug effects/genetics
MH  - Base Sequence
MH  - Calcium Oxalate/*toxicity
MH  - Cell Line
MH  - Cell Proliferation/drug effects/genetics
MH  - Chemokine CCL2/*metabolism
MH  - Crystallization
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Inflammation/*genetics/pathology
MH  - Kidney/metabolism/*pathology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - Nephrolithiasis/genetics
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Up-Regulation/drug effects/genetics
PMC - PMC6933336
OTO - NOTNLM
OT  - HOXA11-AS
OT  - MCP-1
OT  - calcium oxalate
OT  - lncRNA
OT  - mir-124-3p
COIS- The authors confirm that there are no conflicts of interest.
EDAT- 2019/11/05 06:00
MHDA- 2021/03/18 06:00
PMCR- 2020/01/01
CRDT- 2019/11/05 06:00
PHST- 2019/01/15 00:00 [received]
PHST- 2019/08/25 00:00 [revised]
PHST- 2019/09/01 00:00 [accepted]
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
PHST- 2019/11/05 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - JCMM14706 [pii]
AID - 10.1111/jcmm.14706 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2020 Jan;24(1):238-249. doi: 10.1111/jcmm.14706. Epub 2019 Nov 3.