PMID- 31680667
OWN - NLM
STAT- MEDLINE
DCOM- 20200702
LR  - 20200702
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 152
DP  - 2019 Oct 20
TI  - Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of 
      Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues.
LID - 10.3791/60366 [doi]
AB  - Hydatidiform mole (HM) is an abnormal human pregnancy characterized by excessive 
      trophoblastic proliferation and abnormal embryonic development. There are two 
      types of HM based on microscopic morphological evaluation, complete HM (CHM) and 
      partial HM (PHM). These can be further subdivided based on the parental 
      contribution to the molar genomes. Such characterization of HM, by morphology and 
      genotype analyses, is crucial for patient management and for the fundamental 
      understanding of this intriguing pathology. It is well documented that 
      morphological analysis of HM is subject to wide interobserver variability and is 
      not sufficient on its own to accurately classify HM into CHM and PHM and 
      distinguish them from hydropic non-molar abortions. Genotyping analysis is mostly 
      performed on DNA and tissues from formalin-fixed paraffin-embedded (FFPE) 
      products of conception, which have less than optimal quality and may consequently 
      lead to wrong conclusions. In this article, detailed protocols for multiplex 
      genotyping and flow cytometry analyses of FFPE molar tissues are provided, along 
      with the interpretation of the results of these methods, their troubleshooting, 
      and integration with the morphological evaluation, p57(KIP2) 
      immunohistochemistry, and fluorescence in situ hybridization (FISH) to reach a 
      correct and robust diagnosis. Here, the authors share the methods and lessons 
      learned in the past 10 years from the analysis of approximately 400 products of 
      conception.
FAU - Khawajkie, Yassemine
AU  - Khawajkie Y
AD  - Department of Experimental Medicine, McGill University.
FAU - Mechtouf, Nawel
AU  - Mechtouf N
AD  - Department of Human Genetics, McGill University.
FAU - Nguyen, Phuong
AU  - Nguyen P
AD  - Department of Human Genetics, McGill University.
FAU - Slim, Rima
AU  - Slim R
AD  - Department of Experimental Medicine, McGill University; Department of Human 
      Genetics, McGill University; Department of Obstetrics and Gynecology, McGill 
      University; rima.slim@muhc.mcgill.ca.
LA  - eng
GR  - MOP-130364/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20191020
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (CDKN1C protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p57)
SB  - IM
MH  - Cyclin-Dependent Kinase Inhibitor p57/analysis
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Genotyping Techniques/*methods
MH  - Humans
MH  - Hydatidiform Mole/*genetics/pathology
MH  - Microsatellite Repeats/*genetics
MH  - Paraffin Embedding
MH  - *Ploidies
MH  - Pregnancy
MH  - Uterine Neoplasms/*genetics/pathology
EDAT- 2019/11/05 06:00
MHDA- 2020/07/03 06:00
CRDT- 2019/11/05 06:00
PHST- 2019/11/05 06:00 [entrez]
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2020/07/03 06:00 [medline]
AID - 10.3791/60366 [doi]
PST - epublish
SO  - J Vis Exp. 2019 Oct 20;(152). doi: 10.3791/60366.