PMID- 31683196
OWN - NLM
STAT- MEDLINE
DCOM- 20200221
LR  - 20201209
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 127
DP  - 2019 Dec
TI  - TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 
      replication in vitro.
PG  - 105-112
LID - S0034-5288(19)30340-6 [pii]
LID - 10.1016/j.rvsc.2019.10.004 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus 
      (PRRSV), has ranked among the major economically significant pathogen in the 
      global swine industry. The PRRSV nonstructural protein (nsp)11 possesses 
      nidovirus endoribonuclease (NendoU) activity, which is important for virus 
      replication and suppression of the host innate immunity system. Recent proteomic 
      study found that TRIM59 (tripartite motif-containing 59) interacted with the 
      nsp11, albeit the exact role it plays in PRRSV infection remains enigmatic. 
      Herein, we first confirmed the interaction between nsp11 and TRIM59 in 
      co-transfected HEK293T cells and PRRSV-infected pulmonary alveolar macrophages 
      (PAMs). The interacting domains between TRIM59 and nsp11 were further determined 
      to be the N-terminal RING domain in TRIM59 and the C-terminal NendoU domain in 
      nsp11, respectively. Moreover, we reported that overexpression of TRIM59 
      inhibited PRRSV infection in Marc-145 cells. Conversely, small interfering RNA 
      (siRNA) depletion of TRIM59 resulted in enhanced production of PRRSV in PAMs. 
      Together, these data add TRIM59 as a crucial antiviral component against PRRSV 
      and provide new insights for development of new compounds to reduce PRRSV 
      infection.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Jing, Huiyuan
AU  - Jing H
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China. 
      Electronic address: lhsjhy@126.com.
FAU - Ke, Wenting
AU  - Ke W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Tao, Ran
AU  - Tao R
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Li, Yang
AU  - Li Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Zhao, Yabo
AU  - Zhao Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Cao, Sufang
AU  - Cao S
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Sun, Yanting
AU  - Sun Y
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Wang, Jinhe
AU  - Wang J
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Dong, Wang
AU  - Dong W
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Zhao, Pandeng
AU  - Zhao P
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
FAU - Duan, Erzhen
AU  - Duan E
AD  - College of Biological Engineering, Henan university of technology, Zhengzhou 
      450001, PR China.
LA  - eng
PT  - Journal Article
DEP - 20191024
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.- (nidoviral uridylate-specific endoribonuclease)
SB  - IM
MH  - Animals
MH  - Endoribonucleases
MH  - HEK293 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*genetics/metabolism
MH  - Macrophages, Alveolar/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - Tripartite Motif Proteins/*genetics/metabolism
MH  - Viral Nonstructural Proteins/physiology
MH  - Virus Replication/*physiology
OTO - NOTNLM
OT  - Nonstructural protein 11 (nsp11)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Replication
OT  - Tripartite motif 59 (TRIM59)
OT  - Virus-host interactions
EDAT- 2019/11/05 06:00
MHDA- 2020/02/23 06:00
CRDT- 2019/11/05 06:00
PHST- 2019/04/01 00:00 [received]
PHST- 2019/09/27 00:00 [revised]
PHST- 2019/10/06 00:00 [accepted]
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2020/02/23 06:00 [medline]
PHST- 2019/11/05 06:00 [entrez]
AID - S0034-5288(19)30340-6 [pii]
AID - 10.1016/j.rvsc.2019.10.004 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Dec;127:105-112. doi: 10.1016/j.rvsc.2019.10.004. Epub 2019 Oct 
      24.