PMID- 31683569
OWN - NLM
STAT- MEDLINE
DCOM- 20200702
LR  - 20200702
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 11
IP  - 11
DP  - 2019 Oct 31
TI  - Analysis of Swine Conventional Dendritic Cells, DEC205(+)CD172a(+/-)CADM1(+), 
      from Blood and Spleen in Response to PRRSV and PEDV.
LID - 10.3390/v11111001 [doi]
LID - 1001
AB  - Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive 
      and respiratory syndrome virus (PRRSV) but respond to infection via cytokine 
      production, indicating a possible role in initiation/regulation of the immune 
      response against PRRSV. In this work, we evaluated the responses of splenic and 
      blood cDCs, with DEC205(+)CADM1(+)CD172a(+/-) phenotype, as well as those of 
      CD163(+) cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both 
      populations were incubated in the presence of PRRSV or PEDV with and without 
      naive CD3(+) T cells, and cytokine responses were evaluated by qPCR and ELISA. 
      Our results showed that cDCs, but not CD163(+) cells, produced IL-12 in response 
      to PRRSV. PEDV did not induce IL-12 production. Cocultures of cDCs and autologous 
      naive CD3(+) cells resulted in decreased IL-12 production and low expression of 
      IFN-gamma transcripts in response to PRRSV. Interestingly, cDCs increased the 
      proliferation of naive T cells in the presence of PRRSV compared with that 
      achieved with monocytes and peripheral blood mononuclear cells (PBMCs). 
      Cocultures of CD163(+) cells induced IL-10 and IL-4 expression in the presence of 
      PRRSV and PEDV, respectively. In conclusion, cDCs can selectively produce IL-12 
      in response to PRRSV but poorly participate in the activation of naive T cells.
FAU - Parra-Sanchez, Hector
AU  - Parra-Sanchez H
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      parraqh@gmail.com.
FAU - Bustamante-Cordova, Lorena
AU  - Bustamante-Cordova L
AUID- ORCID: 0000-0002-0432-856X
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      lorebcw@gmail.com.
FAU - Resendiz, Monica
AU  - Resendiz M
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      mresendiz@ciad.mx.
FAU - Mata-Haro, Veronica
AU  - Mata-Haro V
AUID- ORCID: 0000-0002-7394-4569
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      vmata@ciad.mx.
FAU - Pinelli-Saavedra, Araceli
AU  - Pinelli-Saavedra A
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      pinelli@ciad.mx.
FAU - Hernandez, Jesus
AU  - Hernandez J
AUID- ORCID: 0000-0002-5131-3600
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C., Kilometro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico. 
      jhdez@ciad.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191031
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Cell Adhesion Molecule-1)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Cell Surface)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Antigens, CD/blood
MH  - Antigens, Differentiation, Myelomonocytic/blood
MH  - Cell Adhesion Molecule-1/blood
MH  - Coronavirus Infections/immunology/*veterinary
MH  - Cytokines/blood
MH  - Dendritic Cells/*immunology/virology
MH  - Interleukin-10/blood
MH  - Interleukin-12/blood
MH  - Interleukin-4/blood
MH  - Leukocytes, Mononuclear/immunology/virology
MH  - Monocytes/immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine epidemic diarrhea virus
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Primary Cell Culture
MH  - Receptors, Cell Surface/blood
MH  - Spleen/cytology/immunology/virology
MH  - Swine
MH  - T-Lymphocyte Subsets/immunology/virology
MH  - *T-Lymphocytes/immunology/virology
PMC - PMC6893809
OTO - NOTNLM
OT  - DEC205
OT  - IL-12
OT  - PEDV
OT  - PRRSV
OT  - cDC
OT  - dendritic cells
OT  - naive T cells
COIS- The authors have no conflicts of interest to declare.
EDAT- 2019/11/07 06:00
MHDA- 2020/07/03 06:00
PMCR- 2019/11/01
CRDT- 2019/11/06 06:00
PHST- 2019/09/26 00:00 [received]
PHST- 2019/10/28 00:00 [revised]
PHST- 2019/10/30 00:00 [accepted]
PHST- 2019/11/06 06:00 [entrez]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/07/03 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - v11111001 [pii]
AID - viruses-11-01001 [pii]
AID - 10.3390/v11111001 [doi]
PST - epublish
SO  - Viruses. 2019 Oct 31;11(11):1001. doi: 10.3390/v11111001.