PMID- 31686194
OWN - NLM
STAT- MEDLINE
DCOM- 20191220
LR  - 20191220
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 475
IP  - 6
DP  - 2019 Dec
TI  - Novel markers in pediatric-type follicular lymphoma.
PG  - 771-779
LID - 10.1007/s00428-019-02681-y [doi]
AB  - The aim of this study was to review the histopathological, phenotypic, and 
      molecular characteristics of pediatric-type follicular lymphoma (PTFL) and to 
      assess the diagnostic value of novel immunohistochemical markers in 
      distinguishing PTFL from follicular hyperplasia (FH). A total of 13 nodal PTFLs 
      were investigated using immunohistochemistry, fluorescence in situ hybridization 
      (FISH), and PCR and were compared with a further 20 reactive lymph nodes showing 
      FH. Morphologically, PTFL cases exhibited a follicular growth pattern with 
      irregular lymphoid follicles in which the germinal centers were composed of 
      numerous blastoid cells showing a starry-sky appearance. Immunohistochemistry 
      highlighted preserved CD10 (13/13) and BCL6 (13/13) staining, CD20 (13/13) 
      positivity, a K light chain predominance (7/13), and partial BCL2 expression in 
      6/13 cases (using antibodies 124, E17, and SP66). The germinal center 
      (GC)-associated markers stathmin and LLT-1 were positive in most of the cases 
      (12/13 and 12/13, respectively). Interestingly, FOXP-1 was uniformly positive in 
      PTFL (12/13 cases) in contrast to reactive GCs in FH, where only a few isolated 
      positive cells were observed. FISH revealed no evidence of BCL2, BCL6, or MYC 
      rearrangements in the examined cases. By PCR, clonal immunoglobulin gene 
      rearrangements were detected in 100% of the tested PTFL cases. Our study 
      confirmed the unique morphological and immunophenotypic features of PTFL and 
      suggests that FOXP-1 can represent a novel useful diagnostic marker in the 
      differential diagnosis between PTFL and FH.
FAU - Agostinelli, Claudio
AU  - Agostinelli C
AD  - Haematopathology Unit, Department of Experimental Diagnostic and Specialty 
      Medicine, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
FAU - Akarca, Ayse U
AU  - Akarca AU
AD  - Department of Pathology, University College London, London, UK.
FAU - Ramsay, Alan
AU  - Ramsay A
AD  - Department of Cellular Pathology, University College Hospital London, London, UK.
FAU - Rizvi, Hasan
AU  - Rizvi H
AD  - Department of Cellular Pathology, Barts Health NHS Trust, London, UK.
FAU - Rodriguez-Justo, Manuel
AU  - Rodriguez-Justo M
AD  - Department of Pathology, University College London, London, UK.
AD  - Department of Cellular Pathology, University College Hospital London, London, UK.
FAU - Pomplun, Sabine
AU  - Pomplun S
AD  - Department of Cellular Pathology, University College Hospital London, London, UK.
FAU - Proctor, Ian
AU  - Proctor I
AD  - Department of Cellular Pathology, University College Hospital London, London, UK.
FAU - Sabattini, Elena
AU  - Sabattini E
AD  - Haematopathology Unit, Department of Experimental Diagnostic and Specialty 
      Medicine, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
FAU - Linch, David
AU  - Linch D
AD  - Department of Haematology, University College London Cancer Institute, London, 
      UK.
FAU - Daw, Stephen
AU  - Daw S
AD  - Children and Young People's Cancer Service, University College Hospital London, 
      London, UK.
FAU - Pittaluga, Stefania
AU  - Pittaluga S
AD  - Haematology section, Laboratory of Pathology, Center for Cancer Research National 
      Cancer Institute, Bethesda, MD, USA.
FAU - Pileri, Stefano A
AU  - Pileri SA
AD  - Division of Haematopathology, European Institute of Oncology, University Hospital 
      of Tubingen, Institute of Pathology, Tubingen, Germany.
FAU - Jaffe, Elaine S
AU  - Jaffe ES
AD  - Haematology section, Laboratory of Pathology, Center for Cancer Research National 
      Cancer Institute, Bethesda, MD, USA.
FAU - Quintanilla-Martinez, Leticia
AU  - Quintanilla-Martinez L
AD  - University Hospital of Tubingen, Institute of Pathology, Tubingen, Germany.
FAU - Marafioti, Teresa
AU  - Marafioti T
AD  - Department of Pathology, University College London, London, UK. 
      t.marafioti@ucl.ac.uk.
AD  - Department of Cellular Pathology, University College Hospital London, London, UK. 
      t.marafioti@ucl.ac.uk.
LA  - eng
GR  - 21198/Associazione Italiana per la Ricerca sul Cancro/
PT  - Journal Article
DEP - 20191104
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (FOXP1 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Repressor Proteins)
RN  - 0 (STMN1 protein, human)
RN  - 0 (Stathmin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Diagnosis, Differential
MH  - Forkhead Transcription Factors/*metabolism
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Immunophenotyping/methods
MH  - Lymphoma, B-Cell/diagnosis/metabolism/*pathology
MH  - Lymphoma, Follicular/diagnosis/*metabolism/*pathology
MH  - Male
MH  - Repressor Proteins/*metabolism
MH  - Stathmin/metabolism
MH  - Young Adult
PMC - PMC6881426
OTO - NOTNLM
OT  - Differential diagnosis
OT  - FOXP-1
OT  - Immunohistochemistry
OT  - Pediatric-type follicular lymphoma
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/11/07 06:00
MHDA- 2019/12/21 06:00
PMCR- 2019/11/04
CRDT- 2019/11/06 06:00
PHST- 2019/03/24 00:00 [received]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/04/29 00:00 [revised]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2019/12/21 06:00 [medline]
PHST- 2019/11/06 06:00 [entrez]
PHST- 2019/11/04 00:00 [pmc-release]
AID - 10.1007/s00428-019-02681-y [pii]
AID - 2681 [pii]
AID - 10.1007/s00428-019-02681-y [doi]
PST - ppublish
SO  - Virchows Arch. 2019 Dec;475(6):771-779. doi: 10.1007/s00428-019-02681-y. Epub 
      2019 Nov 4.