PMID- 31686219 OWN - NLM STAT- MEDLINE DCOM- 20200427 LR - 20200427 IS - 1573-4838 (Electronic) IS - 0957-4530 (Linking) VI - 30 IP - 11 DP - 2019 Nov 4 TI - Diffusion tensor imaging predicting neurological repair of spinal cord injury with transplanting collagen/chitosan scaffold binding bFGF. PG - 123 LID - 10.1007/s10856-019-6322-y [doi] AB - Prognosis and treatment evaluation of spinal cord injury (SCI) are still in the long-term research stage. Prognostic factors for SCI treatment need effective biomarker to assess therapeutic effect. Quantitative diffusion tensor imaging (DTI) may become a potential indicators for assessing SCI repair. However, its correlation with the results of locomotor function recovery and tissue repair has not been carefully studied. The aim of this study was to use quantitative DTI to predict neurological repair of SCI with transplanting collagen/chitosan scaffold binding basic fibroblast growth factor (bFGF). To achieve our research goals, T10 complete transection SCI model was established. Then collagen/chitosan mixture adsorbed with bFGF (CCS/bFGF) were implanted into rats with SCI. At 8 weeks after modeling, implanting CCS/bFGF demonstrated more significant improvements in locomotor function according to Basso-Beattie-Bresnahan (BBB) score, inclined-grid climbing test, and electrophysiological examinations. DTI was carried out to evaluate the repair of axons by diffusion tensor tractgraphy (DTT), fractional anisotropy (FA) and apparent diffusion coefficient (ADC), a numerical measure of relative white matter from the rostral to the caudal. Parallel to locomotor function recovery, the CCS/bFGF group could significantly promote the regeneration of nerve fibers tracts according to DTT, magnetic resonance imaging (MRI), Bielschowsky's silver staining and immunofluorescence staining. Positive correlations between imaging and locomotor function or histology were found at all locations from the rostral to the caudal (P < 0.0001). These results demonstrated that DTI might be used as an effective predictor for evaluating neurological repair after SCI in experimental trails and clinical cases. FAU - Liu, Xiao-Yin AU - Liu XY AUID- ORCID: 0000-0001-9409-4201 AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. AD - Tianjin Medical University, Qixiangtai Road No. 22, Tianjin, 300070, China. FAU - Liang, Jun AU - Liang J AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. FAU - Wang, Yi AU - Wang Y AD - Department of Neurology, Tianjin Hospital of Tianjin, Tianjin, 300211, China. FAU - Zhong, Lin AU - Zhong L AD - Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, China. FAU - Zhao, Chang-Yu AU - Zhao CY AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Wei, Meng-Guang AU - Wei MG AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Wang, Jing-Jing AU - Wang JJ AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Sun, Xiao-Zhe AU - Sun XZ AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Wang, Ke-Qiang AU - Wang KQ AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. FAU - Duan, Jing-Hao AU - Duan JH AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. FAU - Chen, Chong AU - Chen C AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Tu, Yue AU - Tu Y AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Zhang, Sai AU - Zhang S AD - Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China. FAU - Ming, Dong AU - Ming D AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. richardming@tju.edu.cn. FAU - Li, Xiao-Hong AU - Li XH AD - Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China. lixiaohong12@hotmail.com. LA - eng GR - 81771352, 81671222, 81771350, 81772018/he National Nature Scientific Fund of China/ GR - 81771352, 81671222, 81771350, 81772018/he National Nature Scientific Fund of China/ PT - Journal Article DEP - 20191104 PL - United States TA - J Mater Sci Mater Med JT - Journal of materials science. Materials in medicine JID - 9013087 RN - 103107-01-3 (Fibroblast Growth Factor 2) RN - 9007-34-5 (Collagen) RN - 9012-76-4 (Chitosan) SB - IM MH - Animals MH - Blood Vessel Prosthesis MH - Chitosan/chemistry/*therapeutic use MH - Collagen/chemistry/*therapeutic use MH - *Diffusion Tensor Imaging MH - Fibroblast Growth Factor 2/chemistry/*pharmacology MH - Rats MH - Spinal Cord Injuries/*therapy EDAT- 2019/11/07 06:00 MHDA- 2020/04/28 06:00 CRDT- 2019/11/06 06:00 PHST- 2019/05/15 00:00 [received] PHST- 2019/10/16 00:00 [accepted] PHST- 2019/11/06 06:00 [entrez] PHST- 2019/11/07 06:00 [pubmed] PHST- 2020/04/28 06:00 [medline] AID - 10.1007/s10856-019-6322-y [pii] AID - 10.1007/s10856-019-6322-y [doi] PST - epublish SO - J Mater Sci Mater Med. 2019 Nov 4;30(11):123. doi: 10.1007/s10856-019-6322-y.