PMID- 31687714 OWN - NLM STAT- MEDLINE DCOM- 20200416 LR - 20200416 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 10 IP - 11 DP - 2019 Nov 1 TI - Ingestion of Bifidobacterium longum subspecies infantis strain CCFM687 regulated emotional behavior and the central BDNF pathway in chronic stress-induced depressive mice through reshaping the gut microbiota. PG - 7588-7598 LID - 10.1039/c9fo01630a [doi] AB - Increasing evidence points to the effect of the gut microbiota on central nervous system functions. Supplementation of certain microbial strains has been demonstrated to alleviate depressive behaviors and neurological abnormalities. This study took the approach to screen for an anti-depressive Bifidobacterium longum strain from fourteen candidates and systematically verified its effect in a chronic stress-induced depression mice model. B. longum subsp. infantis strain CCFM687 could significantly enhance the biosynthesis of 5-hydroxytryptamine (5-HTP) in vitro in RIN14B cells through up-regulation of the Tph1 gene expression. Administration of CCFM687 in mice significantly improved the scores in behavioral tests and increased the level of 5-HTP and serotonin (5-HT) in the prefrontal cortex (PFC) of the brain. The brain-derived neurotrophic factor (BDNF) in the PFC was also increased, possibly through the 5-HT1A-CREB-BDNF pathway. In addition, CCFM687 alleviated the hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis response and accordingly reversed the peripheral inflammation status. Moreover, the stress-induced structural and functional dysbiosis of the gut microbiome was improved by CCFM687, through increased alpha diversity and abundance of butyrate-producing bacteria, in conjunction with inhibition of pathogenic gene expression. In summary, these results indicate that supplementation of B. longum subsp. infantis strain CCFM687 may prevent the onset of depression from chronic stress, and RIN14B could serve as an efficient cell model for rapid screening of anti-depressive probiotics. FAU - Tian, Peijun AU - Tian P AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn. FAU - Zou, Renying AU - Zou R AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn. FAU - Song, Linhong AU - Song L AD - State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China and University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhang, Xu AU - Zhang X AUID- ORCID: 0000-0003-2481-946X AD - State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China and University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Jiang, Bin AU - Jiang B AD - State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China and University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Wang, Gang AU - Wang G AUID- ORCID: 0000-0003-2007-2143 AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn and International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, P. R. China and (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou 225004, P. R. China. FAU - Lee, Yuan-Kun AU - Lee YK AD - Department of Microbiology & Immunology, National University of Singapore, Singapore 117597, Singapore. FAU - Zhao, Jianxin AU - Zhao J AUID- ORCID: 0000-0003-4516-0799 AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn and (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou 225004, P. R. China. FAU - Zhang, Hao AU - Zhang H AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn and (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou 225004, P. R. China and National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, P. R. China and Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, P. R. China. FAU - Chen, Wei AU - Chen W AUID- ORCID: 0000-0003-3348-4710 AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China and School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. wanggang@jiangnan.edu.cn and National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, P. R. China and Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, P. R. China. LA - eng PT - Journal Article DEP - 20191105 PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 01K63SUP8D (Fluoxetine) RN - 333DO1RDJY (Serotonin) SB - IM MH - Animals MH - Bifidobacterium longum subspecies infantis/*physiology MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Depression/etiology/*therapy MH - Fluoxetine/pharmacology MH - Gastrointestinal Microbiome/*physiology MH - Mice MH - Mice, Inbred C57BL MH - Probiotics MH - Serotonin/metabolism MH - Stress, Physiological/*physiology EDAT- 2019/11/07 06:00 MHDA- 2020/04/17 06:00 CRDT- 2019/11/06 06:00 PHST- 2019/11/07 06:00 [pubmed] PHST- 2020/04/17 06:00 [medline] PHST- 2019/11/06 06:00 [entrez] AID - 10.1039/c9fo01630a [doi] PST - ppublish SO - Food Funct. 2019 Nov 1;10(11):7588-7598. doi: 10.1039/c9fo01630a. Epub 2019 Nov 5.