PMID- 31689951
OWN - NLM
STAT- MEDLINE
DCOM- 20200508
LR  - 20200508
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 11
IP  - 11
DP  - 2019 Nov 4
TI  - The Impact of a Large Bolus Dose of l-leucine and l-isoleucine on Enteroendocrine 
      and Pancreatic Hormones, and Glycemia in Healthy, Inactive Adults.
LID - 10.3390/nu11112650 [doi]
LID - 2650
AB  - BACKGROUND: The ingestion of whey protein and amino acids with carbohydrate (CHO) 
      enhances the release of glucagon-like peptide-1 (GLP-1) and 
      glucose-dependent-insulinotropic peptide (GIP) that promote insulin secretion. It 
      is unknown if L-isoleucine (Ile) and L-leucine (Leu) have this same effect. The 
      purpose of this study was to examine how Ile and Leu influence both GLP-1 and 
      GIP, subsequent pancreatic hormones, and glycemia in healthy, inactive adults. 
      METHODS: Twelve adults (6F/6M; age 27.4 +/- 2 years; BMI 26.3 +/- 2 kg/m(2); lean 
      body mass 53.2 +/- 5 kg; body fat 34.1 +/- 3%) completed four conditions in a 
      randomized, cross-over fashion. Treatments standardized (0.3 g/kg.LBM(-1)) (1) 
      Leu, (2) Ile, (3) Equal (1:1 g) of Leu + Ile, and (4) placebo (Pla, 3.5 g inert 
      stevia) ingested 30 min prior to an oral glucose tolerance test (OGTT). Samples 
      of plasma glucose, insulin, glucagon, GIP(Total), and GLP-1(Active) were 
      assessed. RESULTS: A treatment (p = 0.01) effect comparing Ile vs. Leu (p = 0.02) 
      in GIP(Total). Area under the curve showed an increase in GIP(Total) from Ile 
      compared to Leu and Pla (p = 0.03). No effect was found on GLP-1. The ingestion 
      of Ile prior to CHO augmented GIP concentration greater than Leu or Pla. No 
      correlation was found between GIP, insulin, and glucose between conditions. 
      CONCLUSIONS: Ile impacts GIP concentration, which did not relate to either 
      insulin or glucose concentrations. Neither Ile, nor Leu seem to have an effect on 
      hyperglycemia ingested prior to a CHO drink.
FAU - Newmire, Daniel E
AU  - Newmire DE
AUID- ORCID: 0000-0001-8666-0728
AD  - Exercise Physiology and Biochemistry Lab, Department of Kinesiology, Texas A&M 
      University-Corpus Christi, Corpus Christi, TX 78414, USA. 
      daniel.newmire@tamucc.edu.
FAU - Rivas, Eric
AU  - Rivas E
AD  - Exercise and Thermal Integrative Physiology Laboratory, Department of Kinesiology 
      and Sports Management, Texas Tech University, Lubbock, TX 79409, USA. 
      eric.rivas@ttu.edu.
FAU - Deemer, Sarah E
AU  - Deemer SE
AUID- ORCID: 0000-0002-4758-6796
AD  - Nutrition Obesity Research Center, University of Alabama-Birmingham, Birmingham, 
      AL 35233, USA. sdeemer@uab.edu.
FAU - Willoughby, Darryn S
AU  - Willoughby DS
AD  - Exercise and Biochemical Nutrition Laboratory, Department of Health, Human 
      Performance, Recreation, Baylor University, Waco, TX 76707, USA. 
      Darryn_Willoughby@baylor.edu.
FAU - Ben-Ezra, Victor
AU  - Ben-Ezra V
AD  - Exercise Physiology and Biochemistry Lab, Department of Kinesiology, Texas 
      Woman's University, Denton, TX 76204, USA. VBenEzra@twu.edu.
LA  - eng
GR  - P30 DK056336/DK/NIDDK NIH HHS/United States
GR  - N/A/Texas American College of Sports Medicine SRDA/
PT  - Journal Article
DEP - 20191104
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Blood Glucose)
RN  - 0 (Pancreatic Hormones)
RN  - 04Y7590D77 (Isoleucine)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Adult
MH  - Blood Glucose/*drug effects
MH  - Body Composition
MH  - Dose-Response Relationship, Drug
MH  - Exercise
MH  - Female
MH  - Humans
MH  - Isoleucine/administration & dosage/*pharmacology
MH  - Leucine/administration & dosage/*pharmacology
MH  - Male
MH  - Pancreatic Hormones/*metabolism
PMC - PMC6893504
OTO - NOTNLM
OT  - L-isoleucine
OT  - L-leucine
OT  - glucagon-like peptide 1
OT  - glucose-dependent insulinotropic peptide
OT  - glycemia
COIS- The authors declare no conflict of interests that relate to the content of this 
      manuscript.
EDAT- 2019/11/07 06:00
MHDA- 2020/05/10 06:00
PMCR- 2019/11/01
CRDT- 2019/11/07 06:00
PHST- 2019/09/29 00:00 [received]
PHST- 2019/10/15 00:00 [revised]
PHST- 2019/10/31 00:00 [accepted]
PHST- 2019/11/07 06:00 [entrez]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/05/10 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - nu11112650 [pii]
AID - nutrients-11-02650 [pii]
AID - 10.3390/nu11112650 [doi]
PST - epublish
SO  - Nutrients. 2019 Nov 4;11(11):2650. doi: 10.3390/nu11112650.