PMID- 31690709
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20240229
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 182
IP  - 1
DP  - 2020 Jan
TI  - Phytochrome B Induces Intron Retention and Translational Inhibition of 
      PHYTOCHROME-INTERACTING FACTOR3.
PG  - 159-166
LID - 10.1104/pp.19.00835 [doi]
AB  - The phytochrome B (phyB) photoreceptor stimulates light responses in plants in 
      part by inactivating repressors of light responses, such as 
      PHYTOCHROME-INTERACTING FACTOR3 (PIF3). Activated phyB inhibits PIF3 by rapid 
      protein degradation and decreased transcription. PIF3 protein degradation is 
      mediated by EIN3-BINDING F-BOX PROTEIN (EBF) and LIGHT-RESPONSE BTB (LRB) E3 
      ligases, the latter of which simultaneously targets phyB for degradation. In this 
      study, we show that PIF3 levels are additionally regulated by alternative 
      splicing and protein translation in Arabidopsis (Arabidopsis thaliana). 
      Overaccumulation of photo-activated phyB, which occurs in the mutant defective 
      for LRB genes under continuous red light, induces a specific alternative splicing 
      of PIF3 that results in retention of an intron in the 5' untranslated region of 
      PIF3 mRNA. In turn, the upstream open reading frames contained within this intron 
      inhibit PIF3 protein synthesis. The phyB-dependent alternative splicing of PIF3 
      is diurnally regulated under the short-day light cycle. We hypothesize that this 
      reversible regulatory mechanism may be utilized to fine tune the level of PIF3 
      protein in light-grown plants and may contribute to the oscillation of PIF3 
      protein abundance under the short-day environment.
CI  - (c) 2020 American Society of Plant Biologists. All Rights Reserved.
FAU - Dong, Jie
AU  - Dong J
AUID- ORCID: 0000-0003-0493-1665
AD  - Department of Molecular, Cellular and Developmental Biology, Yale University, New 
      Haven, Connecticut 06520.
FAU - Chen, Haodong
AU  - Chen H
AUID- ORCID: 0000-0001-7228-6341
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agricultural Sciences and School of Life 
      Sciences, Peking University, Beijing 100871, China.
FAU - Deng, Xing Wang
AU  - Deng XW
AUID- ORCID: 0000-0003-0590-8993
AD  - State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center 
      for Life Sciences, School of Advanced Agricultural Sciences and School of Life 
      Sciences, Peking University, Beijing 100871, China.
FAU - Irish, Vivian F
AU  - Irish VF
AUID- ORCID: 0000-0002-2943-6604
AD  - Department of Molecular, Cellular and Developmental Biology, Yale University, New 
      Haven, Connecticut 06520.
FAU - Wei, Ning
AU  - Wei N
AUID- ORCID: 0000-0001-8827-1320
AD  - Department of Molecular, Cellular and Developmental Biology, Yale University, New 
      Haven, Connecticut 06520 ning.wei@yale.edu.
LA  - eng
GR  - R01 GM047850/GM/NIGMS NIH HHS/United States
GR  - R37 GM047850/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20191105
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (LRB3 protein, Arabidopsis)
RN  - 0 (PIF3 protein, Arabidopsis)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
MH  - Alternative Splicing/genetics/physiology
MH  - Arabidopsis/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism
MH  - Gene Expression Regulation, Plant/genetics
MH  - Introns/*genetics
MH  - Phytochrome B/genetics/*metabolism
PMC - PMC6945864
EDAT- 2019/11/07 06:00
MHDA- 2020/09/09 06:00
PMCR- 2019/11/05
CRDT- 2019/11/07 06:00
PHST- 2019/07/10 00:00 [received]
PHST- 2019/10/22 00:00 [accepted]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
PHST- 2019/11/07 06:00 [entrez]
PHST- 2019/11/05 00:00 [pmc-release]
AID - pp.19.00835 [pii]
AID - 201900835R1 [pii]
AID - 10.1104/pp.19.00835 [doi]
PST - ppublish
SO  - Plant Physiol. 2020 Jan;182(1):159-166. doi: 10.1104/pp.19.00835. Epub 2019 Nov 
      5.