PMID- 31693458
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20200727
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 32
IP  - 9
DP  - 2019 Nov
TI  - Recombinant Porcine Interferon Alpha Enhances Immune Responses to Killed Porcine 
      Reproductive and Respiratory Syndrome Virus Vaccine in Pigs.
PG  - 383-392
LID - 10.1089/vim.2019.0092 [doi]
AB  - In this study, the immunoadjuvant effects of recombinant porcine interferon alpha 
      (rPoIFNalpha) on the killed virus vaccine (KV) of porcine reproductive and 
      respiratory syndrome virus (PRRSV) in pigs were investigated. The experimental 
      pigs were divided into six groups, including normal control group, rPoIFNalpha 
      control group, PRRSV KV control group, KV+40,000 U rPoIFNalpha immunization group, 
      KV+400,000 U rPoIFNalpha immunization group, and KV+4,000,000 U rPoIFNalpha immunization 
      group. The experimental pigs were boosted immunized on the 28th day after the 
      initial immunization, and the heparinized blood and serum samples were collected 
      at different time points of these two immunizations to detect and evaluate the 
      immune responses of pigs after immunization by ELISA assay, neutralization assay, 
      flow cytometry, and so on. The results showed that the proportion of the levels 
      of PRRSV-specific antibodies, neutralizing antibodies, stimulation index, IL-4, 
      IFN-gamma, and lymphocytes within the groups immunized with KV+rPoIFNalpha were 
      significantly higher than that group immunized with KV alone. The humoral and 
      cellular immune responses in pigs were markedly enhanced by rPoIFNalpha after the 
      coadministration with KV vaccine. Therefore, we tentatively think that rPoIFNalpha is 
      a potential immune promoter with prospects for future applications in the pig 
      industry.
FAU - Yu, Hai-Yang
AU  - Yu HY
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
FAU - Qu, Ming-Sheng
AU  - Qu MS
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Zhang, Jun-Ling
AU  - Zhang JL
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Gan, Lin
AU  - Gan L
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Zhao, Yu
AU  - Zhao Y
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Shan, Xue-Qin
AU  - Shan XQ
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Zhou, Wei
AU  - Zhou W
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Xia, Bing-Bing
AU  - Xia BB
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Chen, Jason
AU  - Chen J
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Department of Pathology and Cell Biology, Columbia University, New York, New 
      York.
FAU - Wang, Ming-Li
AU  - Wang ML
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
FAU - Zhao, Jun
AU  - Zhao J
AD  - Department of Microbiology, Anhui Medical University, Hefei, Anhui Province, 
      China.
AD  - Anhui JiuChuan Biotech Co., Ltd., Wuhu, Anhui Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Interferon-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Animals
MH  - Antibodies, Neutralizing/blood/isolation & purification
MH  - Antibodies, Viral/blood/isolation & purification
MH  - *Immunogenicity, Vaccine
MH  - Interferon-alpha/*administration & dosage/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Recombinant Proteins/administration & dosage
MH  - Sus scrofa
MH  - Swine
MH  - Vaccination/methods/veterinary
MH  - Vaccines, Inactivated/administration & dosage/immunology
MH  - Viral Vaccines/administration & dosage/*immunology
OTO - NOTNLM
OT  - adjuvant
OT  - cellular immune response
OT  - humoral immune response
OT  - killed virus vaccine (KV)
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - recombinant porcine interferon alpha
EDAT- 2019/11/07 06:00
MHDA- 2020/07/28 06:00
CRDT- 2019/11/07 06:00
PHST- 2019/11/07 06:00 [entrez]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
AID - 10.1089/vim.2019.0092 [doi]
PST - ppublish
SO  - Viral Immunol. 2019 Nov;32(9):383-392. doi: 10.1089/vim.2019.0092.