PMID- 31694744
OWN - NLM
STAT- MEDLINE
DCOM- 20200917
LR  - 20200917
IS  - 0392-856X (Print)
IS  - 0392-856X (Linking)
VI  - 38
IP  - 4
DP  - 2020 Jul-Aug
TI  - Soluble IL-6R promotes chondrogenic differentiation of mesenchymal stem cells to 
      enhance the repair of articular cartilage defects using a rat model for 
      rheumatoid arthritis.
PG  - 670-679
AB  - OBJECTIVES: Although articular cartilage contributes to smooth joint motion, once 
      damaged this functionality cannot be recovered. Activation of the IL-6/STAT3 
      signalling pathway contributes to chondrogenic differentiation of mesenchymal 
      stem cells (MSCs), indicating a role for soluble IL-6R (sIL-6R) during 
      chondrogenesis in vitro. The aim of this study is to develop a novel therapeutic 
      tool for regenerative medicine of articular cartilage. METHODS: Human bone 
      marrow-derived MSCs were pre-treated with sIL-6R to direct their differentiation 
      into chondrocytes, then seeded on a poly-lactic-co-glycolic acid (PLGA) sheet to 
      enhance the localised residence of MSCs. The material was implanted into knee 
      joint spaces of antigen-induced arthritis (AIA) rats, an animal model of 
      rheumatoid arthritis (RA). After 8 weeks, the effects of the implantation on 
      articular cartilage repair were assessed by x-ray image and staining with 
      safranin O (S-O), aggrecan and human leukocyte antigen (HLA). RESULTS: Swelling 
      of knees in AIA rats, but not sham-treated rats, was observed. AIA rats implanted 
      with PLGA and sIL-6R-treated MSCs showed similar knee joint imaging to sham rats 
      using x-ray; however, those with PLGA alone, or with PLGA with MSCs, did not. 
      Rats implanted with PLGA and sIL-6R-treated MSCs, but not PLGA alone or PLGA with 
      MSCs, showed positive imaging by S-O staining as well as human aggrecan. HLA was 
      not detected in the knees of any of the rats. CONCLUSIONS: PLGA and 
      sIL-6R-treated MSCs help to repair articular cartilage with high efficacy. Thus, 
      the application of this promising strategy to regenerative medicine for articular 
      cartilage in patients with RA is anticipated.
FAU - Yamagata, Kaoru
AU  - Yamagata K
AD  - The First Department of Internal Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Nakayamada, Shingo
AU  - Nakayamada S
AD  - The First Department of Internal Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Zhang, Tong
AU  - Zhang T
AD  - The First Department of Internal Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Zhang, Xiangmei
AU  - Zhang X
AD  - Research Center, The Fourth Hospital of Hebei Medical University and The Tumor 
      Hospital of Hebei Province, Shijiazhuang, China.
FAU - Tanaka, Yoshiya
AU  - Tanaka Y
AD  - The First Department of Internal Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan. tanaka@med.uoeh-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20191030
PL  - Italy
TA  - Clin Exp Rheumatol
JT  - Clinical and experimental rheumatology
JID - 8308521
SB  - IM
MH  - Animals
MH  - *Arthritis, Rheumatoid
MH  - *Cartilage, Articular
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrogenesis
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Rats
EDAT- 2019/11/07 06:00
MHDA- 2020/09/18 06:00
CRDT- 2019/11/08 06:00
PHST- 2019/05/22 00:00 [received]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/09/18 06:00 [medline]
PHST- 2019/11/08 06:00 [entrez]
AID - 14340 [pii]
PST - ppublish
SO  - Clin Exp Rheumatol. 2020 Jul-Aug;38(4):670-679. Epub 2019 Oct 30.