PMID- 31696493
OWN - NLM
STAT- MEDLINE
DCOM- 20201013
LR  - 20201013
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 20
DP  - 2019 Oct
TI  - Correlation between endothelial cell apoptosis and SIRT3 gene expression in 
      atherosclerosis rats.
PG  - 9033-9040
LID - 19305 [pii]
LID - 10.26355/eurrev_201910_19305 [doi]
AB  - OBJECTIVE: To investigate the correlation between the endothelial cell apoptosis 
      and sirtuin-3 (SIRT3) gene expression in atherosclerosis (AS) rats. MATERIALS AND 
      METHODS: The AS model in rats was established through the high-fat diet. A total 
      of 12 rats fed normally were enrolled as the control group, while 12 rats fed 
      with high-fat diet were enrolled as the experimental group. After the experiment, 
      the aortic tissues of rats were collected, and the relative area of the arterial 
      plaque (total area of plaque/total area of the vessel) was measured via oil red O 
      staining. The serum was collected to detect the levels of blood lipid, including 
      total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol 
      (LDL-C) and high-density lipoprotein cholesterol (HDL-C). Moreover, the 
      expression levels of SIRT3 and apoptotic genes were determined via Reverse 
      Transcription-Polymerase Chain Reaction (RT-PCR), Western blotting and 
      immunohistochemistry (IHC), respectively. The apoptosis was detected via terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. 
      RESULTS: The area of aortic plaque in the experimental group [(36.15+/-9.52)%] was 
      significantly larger than that in the control group [(11.62+/-3.25)%] (p<0.01). 
      Compared with those in the control group, the serum TC, TG and LDL-C levels were 
      significantly increased in the experimental group, while the HDL-C level was 
      significantly decreased (p<0.05). Compared with those in the control group, the 
      mRNA and protein expression levels of SIRT3 in the aorta of rats markedly 
      declined in the experimental group (p<0.05), while Caspase-3 and Caspase-9 
      expressions were significantly increased (p<0.05), respectively. The results of 
      TUNEL staining revealed that the apoptosis in the aorta of rats in the 
      experimental group was remarkably higher than that in the control group (p<0.05). 
      CONCLUSIONS: The expression of SIRT3 is deleted in the aorta of AS rats and 
      closely related to the apoptosis. SIRT3 may serve as a potential target for the 
      treatment of AS.
FAU - Jing, S-H
AU  - Jing SH
AD  - Department of Cardiovascular, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, China. jingshenhong7915@163.com.
FAU - Yu, B
AU  - Yu B
FAU - Qiao, H
AU  - Qiao H
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Cholesterol, HDL)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (SIRT3 protein, rat)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.5.1.- (Sirtuins)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Atherosclerosis/chemically induced/genetics/metabolism/*pathology
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Cholesterol/blood
MH  - Cholesterol, HDL/blood
MH  - Cholesterol, LDL/blood
MH  - Diet, High-Fat/*adverse effects
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Endothelial Cells/*cytology/drug effects
MH  - Male
MH  - Rats
MH  - Sirtuins/*genetics/*metabolism
MH  - Triglycerides/blood
EDAT- 2019/11/07 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/11/08 06:00
PHST- 2019/11/08 06:00 [entrez]
PHST- 2019/11/07 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 19305 [pii]
AID - 10.26355/eurrev_201910_19305 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):9033-9040. doi: 
      10.26355/eurrev_201910_19305.