PMID- 31701529
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20210114
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 594
IP  - 6
DP  - 2020 Mar
TI  - Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels 
      in Arabidopsis chloroplasts.
PG  - 986-994
LID - 10.1002/1873-3468.13664 [doi]
AB  - Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in 
      the chloroplast. Although the regulation of ROS in chloroplasts is well 
      elucidated, the precise regulation mechanism of Trx remains unknown. Here, we 
      characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), 
      which contains only a Lon domain, the precise function of which is not known. We 
      find that LCP1 interacts with Trx-y2 and represses its activity, and that 
      knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin 
      deposition. In addition, LCP1 KD plants show less ROS accumulation and lower 
      expression of ROS-responsive marker genes than the wild-type plant. Taken 
      together, we suggest that LCP1 directly regulates Trx-y2 and controls H(2) O(2) 
      levels and, thereby, regulates lignin polymerization in the anther endothecium.
CI  - (c) 2019 Federation of European Biochemical Societies.
FAU - Shin, Jin Seok
AU  - Shin JS
AUID- ORCID: 0000-0002-3617-7954
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
FAU - Kim, Soo Youn
AU  - Kim SY
AUID- ORCID: 0000-0002-4498-4799
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
AD  - Bionics Inc., Seongdong-gu, Seoul, Korea.
FAU - So, Won Mi
AU  - So WM
AUID- ORCID: 0000-0003-4572-3248
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
FAU - Noh, Minsoo
AU  - Noh M
AUID- ORCID: 0000-0002-1239-3783
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
FAU - Yoo, Kyoung Shin
AU  - Yoo KS
AUID- ORCID: 0000-0002-5519-5568
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX, USA.
FAU - Shin, Jeong Sheop
AU  - Shin JS
AUID- ORCID: 0000-0002-5944-4947
AD  - Division of Life Sciences, Korea University, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191122
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Arabidopsis Proteins)
RN  - 52500-60-4 (Thioredoxins)
RN  - 9005-53-2 (Lignin)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Arabidopsis/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Flowers/genetics/*metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Hydrogen Peroxide/metabolism
MH  - Lignin/biosynthesis/genetics
MH  - Thioredoxins/genetics/*metabolism
OTO - NOTNLM
OT  - Arabidopsis
OT  - ROS
OT  - chloroplast
OT  - dehiscence
OT  - lignification
OT  - thioredoxin
EDAT- 2019/11/09 06:00
MHDA- 2021/01/15 06:00
CRDT- 2019/11/09 06:00
PHST- 2019/09/16 00:00 [received]
PHST- 2019/10/11 00:00 [revised]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/11/09 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
PHST- 2019/11/09 06:00 [entrez]
AID - 10.1002/1873-3468.13664 [doi]
PST - ppublish
SO  - FEBS Lett. 2020 Mar;594(6):986-994. doi: 10.1002/1873-3468.13664. Epub 2019 Nov 
      22.