PMID- 31701719
OWN - NLM
STAT- MEDLINE
DCOM- 20191118
LR  - 20200108
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 35
IP  - 4
DP  - 2019 Jul 28
TI  - [Effects of endurance exercise on synaptic plasticity in cerebral cortex of aged 
      rats and related regulatory mechanism].
PG  - 339-345
LID - 10.12047/j.cjap.5803.2019.072 [doi]
AB  - OBJECTIVE: To understand and analyze the rules of endurance exercise on the 
      cerebral cortex adaptive mechanism in aged rats. METHODS: In this study, 
      3-month-old (n=20), 13-month-old (n=24) and 23-month-old (n=24) specific-pathogen 
      free (SPF) male Sprague-Dawley Rat (SD) rats were divided into young (Y-SED), 
      middle-aged (M-SED) and old-aged (O-SED) sedentary control group, and the 
      corresponding Y-EX, M-EX and O-EX in the endurance exercise runner group. The 
      10-weeks of regular moderate-intensity aerobic exercise intervention were carried 
      out in the endurance exercise runner group. The exercise mode is treadmill 
      exercise (slope 0), and the exercise intensity gradually increases from 60%～65% 
      of the maximum oxygen consumption (V.O(2max)) to 70%～75%, and the exercise time 
      is 10 weeks. Hematoxylin and eosin (HE) staining was used to detect age-related 
      morphological changes. The expressions of superoxide dismutase(SOD) and 
      brain-derived neurotrophic factor (BDNF) and the expressions of synapsin 1 (SYN1) 
      and Ca(2+)/calmodulin- dependent protein kinases IIalpha (CaMK IIalpha) / AMP-activated 
      protein kinase alpha1(AMPKalpha1) / mammalian target of rapamycin (mTOR) pathway -related 
      genes were detected. RESULTS: The cerebral cortex structure of the rats in each 
      group showed age-related aging changes, the expression of SOD in the cortex 
      showed a gradual decline, the expression of BDNF showed an age-increasing trend, 
      and the expression levels of SYN1 and CaMK IIalpha were increased with age. The 
      changes in AMPKalpha1 and SirT2 and IP3R, AKT1 and mTOR mRNA levels were increased 
      slightly in middle-aged rats and decreased in aged rats. Compared with the rats 
      in each sedentary control group, the nucleus of the cerebral cortex was tightly 
      arranged and the number of nuclei observed under the microscope was increased 
      significantly in each exercise group. Exercise promoted the expressions of SOD, 
      BDNF and synaptophysin SYN1 in the cortex of rats, and the expression levels of 
      SOD and BDNF in aged rats were up-regulated significantly (P＜ 0.01). The 
      expression level of SYN1 in rats was up-regulated significantly (P＜0.05) in the 
      young and aged rats. The expression of CaMK IIalpha in the cortex of middle-aged and 
      aged rats was up-regulated (P＜0.01), while the expression level of CaMK IIalpha in 
      young rats was down-regulated (P＜0.01). Exercise could up-regulate the expression 
      level of AMPKalpha1 in the cortex of young rats (P＜ 0.05), but not in middle-aged and 
      old-age rats. Exercise could up-regulate the expression of SirT2 in the cortex of 
      rats in all age groups (P＜0.05). Exercise up-regulated the expression of 
      phosphoinositide 3-kinase (IP3R)/ protein kinase B 1(AKT1) /mTOR in the cortex of 
      rats, among which young IP3R was significantly up-regulated (P＜0.01) in the young 
      group, mTOR was significantly up-regulated in young and middle-aged group 
      (P＜0.01), and mTOR was also significantly up-regulated in the aged group 
      (P＜0.05). CONCLUSION: Endurance exercise up-regulates BDNF expression, regulates 
      CaMKIIalpha signaling, activates AMPK signaling pathway and IP3R / AKT1 / mTOR 
      signaling pathway, and improves synaptic plasticity in the cortex.
FAU - Liu, Wen-Feng
AU  - Liu WF
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Liu, Shao-Peng
AU  - Liu SP
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Fu, Rang
AU  - Fu R
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Wang, Zhi-Yuan
AU  - Wang ZY
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Kuang, He-Yu
AU  - Kuang HY
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Xia, Yan
AU  - Xia Y
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
FAU - Tang, Chang-Fa
AU  - Tang CF
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University, Changsha 410012, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Cerebral Cortex/*physiology
MH  - Male
MH  - *Neuronal Plasticity
MH  - *Physical Conditioning, Animal
MH  - *Physical Endurance
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
EDAT- 2019/11/09 06:00
MHDA- 2019/11/19 06:00
CRDT- 2019/11/09 06:00
PHST- 2019/11/09 06:00 [entrez]
PHST- 2019/11/09 06:00 [pubmed]
PHST- 2019/11/19 06:00 [medline]
AID - 10.12047/j.cjap.5803.2019.072 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2019 Jul 28;35(4):339-345. doi: 
      10.12047/j.cjap.5803.2019.072.