PMID- 31702026
OWN - NLM
STAT- MEDLINE
DCOM- 20200331
LR  - 20200331
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 5
DP  - 2019 Nov
TI  - Knockdown of long noncoding RNA DLEU1 suppresses the progression of renal cell 
      carcinoma by downregulating the Akt pathway.
PG  - 4551-4557
LID - 10.3892/mmr.2019.10705 [doi]
AB  - Increasing evidence has indicated that long noncoding RNAs (lncRNAs) are involved 
      in the tumorigenesis and progression of various types of cancer. The lncRNA 
      deleted in lymphocytic leukemia 1 (DLEU1) has been reported to be dysregulated in 
      cancer cells and thus associated with tumor development; however, the role of 
      DLEU1 in renal cell carcinoma (RCC) remains unclear. In the present study, DLEU1 
      was knocked down using small interfering RNA in the RCC cell lines KETR3 and 
      786‑O to determine the role of DLEU1. Cell Counting Kit‑8, colony formation, 
      Transwell and flow cytometry assays were performed to assess the effects of DLEU1 
      on cell proliferation, migration, invasion and apoptosis in KETR3 and 786‑O 
      cells. The protein expression levels of factors associated with apoptosis and 
      epithelial‑mesenchymal transition (EMT) were examined by western blot. The 
      results demonstrated that silencing DLEU1 decreased the growth capacity, 
      migration and invasion of KETR3 and 786‑O cells. Additionally, loss of DLEU1 was 
      observed to stimulate the mitochondrial pathway of cell apoptosis via regulation 
      of the expression of Bcl‑2/Bax, cleaved caspase‑3 and cleaved caspase‑9 in KETR3 
      and 786‑O cells. Furthermore, DLEU1 knockdown significantly inhibited the protein 
      kinase B (Akt) pathway by downregulating the expression of phosphorylated‑Akt, 
      cyclin  D1 and P70S6 kinase. In addition, depletion of DLEU1 was observed to 
      impair the process of EMT in RCC cells via the upregulation of E‑cadherin, and 
      downregulation of N‑cadherin and vimentin. Collectively, these results indicated 
      a pro‑oncogenic role of DLEU1 in the progression and development of RCC via 
      modulation of the Akt pathway and EMT phenotype.
FAU - Yue, Genquan
AU  - Yue G
AD  - Department of Urology, The Affiliated Hospital of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Chen, Caixia
AU  - Chen C
AD  - Clinical Medical Research Center, The Affiliated Hospital of Inner Mongolia 
      Medical University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Bai, Ligang
AU  - Bai L
AD  - Department of Urology, The Affiliated Hospital of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Wang, Guoqiang
AU  - Wang G
AD  - Department of Urology, The Affiliated Hospital of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Huang, Yong
AU  - Huang Y
AD  - Department of Urology, The Affiliated Hospital of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Wang, Yunbin
AU  - Wang Y
AD  - Department of Urology, The Affiliated Hospital of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Cui, Hongwei
AU  - Cui H
AD  - Clinical Medical Research Center, The Affiliated Hospital of Inner Mongolia 
      Medical University, Hohhot, Inner Mongolia 010000, P.R. China.
FAU - Xiao, Yunfeng
AU  - Xiao Y
AD  - The Center for New Drug Safety Evaluation and Research of Inner Mongolia Medical 
      University, Hohhot, Inner Mongolia 010110, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190925
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Carcinoma, Renal Cell/genetics/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - *Down-Regulation
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Kidney Neoplasms/genetics/*metabolism/pathology
MH  - Proto-Oncogene Proteins c-akt/*biosynthesis/genetics
MH  - RNA, Long Noncoding/*biosynthesis/genetics
MH  - RNA, Neoplasm/*biosynthesis/genetics
MH  - *Signal Transduction
PMC - PMC6797951
EDAT- 2019/11/09 06:00
MHDA- 2020/04/01 06:00
PMCR- 2019/09/25
CRDT- 2019/11/09 06:00
PHST- 2018/10/18 00:00 [received]
PHST- 2019/04/09 00:00 [accepted]
PHST- 2019/11/09 06:00 [pubmed]
PHST- 2020/04/01 06:00 [medline]
PHST- 2019/11/09 06:00 [entrez]
PHST- 2019/09/25 00:00 [pmc-release]
AID - mmr-20-05-4551 [pii]
AID - 10.3892/mmr.2019.10705 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Nov;20(5):4551-4557. doi: 10.3892/mmr.2019.10705. Epub 2019 Sep 
      25.