PMID- 31702786
OWN - NLM
STAT- MEDLINE
DCOM- 20210202
LR  - 20240329
IS  - 1464-3804 (Electronic)
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 35
IP  - 1
DP  - 2020 Feb 13
TI  - Urinary N7-(1-hydroxy-3-buten-2-yl) guanine adducts in humans: temporal stability 
      and association with smoking.
PG  - 19-26
LID - 10.1093/mutage/gez030 [doi]
AB  - 1,3-Butadiene (BD) is a known human carcinogen found in cigarette smoke, 
      automobile exhaust, and urban air. Workers occupationally exposed to BD in the 
      workplace have an increased incidence of leukemia and lymphoma. BD undergoes 
      cytochrome P450-mediated metabolic activation to 3,4-epoxy-1-butene (EB), 
      1,2,3,4-diepoxybutane (DEB) and 1,2-dihydroxy-3,4-epoxybutane (EBD), which form 
      covalent adducts with DNA. We have previously reported a quantitative 
      nanoLC/ESI+-HRMS3 method for urinary N7-(1-hydroxy-3-buten-2-yl) guanine (EB-GII) 
      adducts as a mechanism-based biomarker of BD exposure. In the present study, the 
      method was updated to include high throughput 96-well solid phase extraction 
      (SPE) and employed to establish urinary EB-GII biomarker stability and 
      association with smoking. Urinary EB-GII levels were measured bimonthly for 1 
      year in 19 smokers to determine whether single adduct measurement provides 
      reliable levels of EB-GII in an individual smoker. In addition, association of 
      EB-GII with smoking was studied in 17 individuals participating in a smoking 
      cessation program. EB-GII levels decreased 34% upon smoking cessation, indicating 
      that it is associated with smoking status, but may also originate from sources 
      other than exposure to cigarette smoke.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of the UK 
      Environmental Mutagen Society. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Jokipii Krueger, Caitlin C
AU  - Jokipii Krueger CC
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
FAU - Madugundu, Guru
AU  - Madugundu G
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
FAU - Degner, Amanda
AU  - Degner A
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
FAU - Patel, Yesha
AU  - Patel Y
AD  - Department of Preventative Medicine, School of Medicine, University of Southern 
      California, Los Angeles, CA.
FAU - Stram, Daniel O
AU  - Stram DO
AD  - Department of Preventative Medicine, School of Medicine, University of Southern 
      California, Los Angeles, CA.
FAU - Church, Timothy R
AU  - Church TR
AD  - Division of Environmental Health Sciences, University of Minnesota School of 
      Public Health, Minneapolis, MN.
FAU - Tretyakova, Natalia
AU  - Tretyakova N
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
LA  - eng
GR  - P01 CA138338/CA/NCI NIH HHS/United States
GR  - R01 CA100670/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Butadienes)
RN  - 0 (Carcinogens)
RN  - 0 (DNA Adducts)
RN  - 0 (N-7-(1-hydroxy-3-buten-2-yl)guanine)
RN  - 5Z93L87A1R (Guanine)
RN  - JSD5FGP5VD (1,3-butadiene)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/urine
MH  - Butadienes/metabolism
MH  - Carcinogens/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - DNA Adducts/isolation & purification/metabolism/*urine
MH  - Female
MH  - Guanine/isolation & purification/urine
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Smoking/ethnology/*urine
MH  - Smoking Prevention
MH  - Solid Phase Extraction
MH  - Spectrometry, Mass, Electrospray Ionization
PMC - PMC7016204
EDAT- 2019/11/09 06:00
MHDA- 2021/02/03 06:00
PMCR- 2021/02/13
CRDT- 2019/11/09 06:00
PHST- 2019/05/16 00:00 [received]
PHST- 2019/09/13 00:00 [accepted]
PHST- 2019/11/09 06:00 [pubmed]
PHST- 2021/02/03 06:00 [medline]
PHST- 2019/11/09 06:00 [entrez]
PHST- 2021/02/13 00:00 [pmc-release]
AID - 5614980 [pii]
AID - gez030 [pii]
AID - 10.1093/mutage/gez030 [doi]
PST - ppublish
SO  - Mutagenesis. 2020 Feb 13;35(1):19-26. doi: 10.1093/mutage/gez030.