PMID- 31703129
OWN - NLM
STAT- MEDLINE
DCOM- 20191120
LR  - 20200108
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 36
IP  - 11
DP  - 2019 Nov 10
TI  - [Application of multiple MLL gene rearrangement detection techniques for children 
      with acute mononuclear leukemia].
PG  - 1077-1080
LID - 10.3760/cma.j.issn.1003-9406.2019.11.005 [doi]
AB  - OBJECTIVE: To assess the value of detecting multiple rearrangements of MLL gene 
      in children with acute mononuclear leukemia (AML). METHODS: Eighty six children 
      with AML were analyzed by fluorescence in situ hybridization (FISH), chromosomal 
      karyotyping and multiplex reverse transcription-PCR (RT-PCR). RESULTS: Cross 
      signals were detected by FISH in 26 cases, and 30.2% were detected with MLL gene 
      rearrangements. R-band karyotyping analysis revealed 14 translocations with 
      breakages involving 11q23 and 5 other aberrations, which yielded an overall 
      detection rate of 22.1%. Multiple RT-PCR has detected 12 fusion genes produced by 
      the MLL translocation, which yielded a detection rate of 14.0%. A significant 
      difference was found in the detection rate of the three methods (P< 0.05). 
      CONCLUSION: Combined use of FISH, chromosomal karyotyping and multiplex RT-PCR 
      can improve the detection of MLL gene rearrangements and provide important clues 
      for clinical diagnosis, treatment and prognosis of AML.
FAU - Zhao, Ding
AU  - Zhao D
AD  - Henan Provincial Key Laboratory for Genetics and Metabolic Diseases in Childhood, 
      Children's Hospital Affiliated of Zhengzhou University, Henan Children's 
      Hospital, Zhengzhou Children's Hospital, Zhengzhou, Henan 450018, China. 
      15003998000@139.com.
FAU - Li, Rui
AU  - Li R
FAU - Zhang, Yaodong
AU  - Zhang Y
FAU - Song, Jijun
AU  - Song J
FAU - Zhang, Zhenhua
AU  - Zhang Z
FAU - Wang, Meiye
AU  - Wang M
FAU - Wang, Chaojie
AU  - Wang C
FAU - Wang, Jin
AU  - Wang J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (KMT2A protein, human)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Child
MH  - Chromosomes, Human, Pair 11
MH  - Gene Rearrangement
MH  - Histone-Lysine N-Methyltransferase/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Myeloid-Lymphoid Leukemia Protein/*genetics
MH  - Translocation, Genetic
EDAT- 2019/11/09 06:00
MHDA- 2019/11/21 06:00
CRDT- 2019/11/09 06:00
PHST- 2019/11/09 06:00 [entrez]
PHST- 2019/11/09 06:00 [pubmed]
PHST- 2019/11/21 06:00 [medline]
AID - 940636227 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2019.11.005 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2019 Nov 10;36(11):1077-1080. doi: 
      10.3760/cma.j.issn.1003-9406.2019.11.005.