PMID- 31703698
OWN - NLM
STAT- MEDLINE
DCOM- 20200910
LR  - 20211204
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 17
IP  - 1
DP  - 2019 Nov 8
TI  - Epitope-based peptide vaccine design and target site depiction against Middle 
      East Respiratory Syndrome Coronavirus: an immune-informatics study.
PG  - 362
LID - 10.1186/s12967-019-2116-8 [doi]
LID - 362
AB  - BACKGROUND: Middle East Respiratory Syndrome Coronavirus (MERS-COV) is the main 
      cause of lung and kidney infections in developing countries such as Saudi Arabia 
      and South Korea. This infectious single-stranded, positive (+) sense RNA virus 
      enters the host by binding to dipeptidyl-peptide receptors. Since no vaccine is 
      yet available for MERS-COV, rapid case identification, isolation, and infection 
      prevention strategies must be used to combat the spreading of MERS-COV infection. 
      Additionally, there is a desperate need for vaccines and antiviral strategies. 
      METHODS: The present study used immuno-informatics and computational approaches 
      to identify conserved B- and T cell epitopes for the MERS-COV spike (S) protein 
      that may perform a significant role in eliciting the resistance response to 
      MERS-COV infection. RESULTS: Many conserved cytotoxic T-lymphocyte epitopes and 
      discontinuous and linear B-cell epitopes were predicted for the MERS-COV S 
      protein, and their antigenicity and interactions with the human leukocyte antigen 
      (HLA) B7 allele were estimated. Among B-cell epitopes, QLQMGFGITVQYGT displayed 
      the highest antigenicity-score, and was immensely immunogenic. Among T-cell 
      epitopes, MHC class-I peptide YKLQPLTFL and MHC class-II peptide YCILEPRSG were 
      identified as highly antigenic. Furthermore, docking analyses revealed that the 
      predicted peptides engaged in strong bonding with the HLA-B7 allele. CONCLUSION: 
      The present study identified several MERS-COV S protein epitopes that are 
      conserved among various isolates from different countries. The putative antigenic 
      epitopes may prove effective as novel vaccines for eradication and combating of 
      MERS-COV infection.
FAU - Tahir Ul Qamar, Muhammad
AU  - Tahir Ul Qamar M
AUID- ORCID: 0000-0003-4832-4250
AD  - College of Informatics, Huazhong Agricultural University, Wuhan, People's 
      Republic of China. m.tahirulqamar@webmail.hzau.edu.cn.
FAU - Saleem, Saman
AU  - Saleem S
AD  - Department of Bioinformatics and Biotechnology, Government College University 
      Faisalabad, Faisalabad, Pakistan.
FAU - Ashfaq, Usman Ali
AU  - Ashfaq UA
AD  - Department of Bioinformatics and Biotechnology, Government College University 
      Faisalabad, Faisalabad, Pakistan.
FAU - Bari, Amna
AU  - Bari A
AD  - College of Informatics, Huazhong Agricultural University, Wuhan, People's 
      Republic of China.
FAU - Anwar, Farooq
AU  - Anwar F
AD  - Department of Chemistry, University of Sargodha, Sargodha, Pakistan.
FAU - Alqahtani, Safar
AU  - Alqahtani S
AD  - Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin 
      Abdul Aziz University, Alkharj, Saudi Arabia. safar.alqahtani@psau.edu.sa.
LA  - eng
PT  - Journal Article
DEP - 20191108
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Viral/chemistry/genetics/immunology
MH  - Coronavirus Infections/*immunology/*prevention & control/virology
MH  - Epitopes, B-Lymphocyte/chemistry/genetics/immunology
MH  - Epitopes, T-Lymphocyte/chemistry/genetics/immunology
MH  - Genome, Viral
MH  - HLA Antigens/chemistry/genetics
MH  - Host Microbial Interactions/genetics/immunology
MH  - Humans
MH  - Middle East Respiratory Syndrome Coronavirus/chemistry/genetics/*immunology
MH  - Models, Molecular
MH  - Molecular Docking Simulation
MH  - Republic of Korea
MH  - Saudi Arabia
MH  - Spike Glycoprotein, Coronavirus/chemistry/genetics/immunology
MH  - Translational Research, Biomedical
MH  - Vaccines, Subunit/chemistry/genetics/immunology
MH  - Viral Vaccines/chemistry/genetics/immunology
PMC - PMC6839065
OTO - NOTNLM
OT  - Computational approaches
OT  - MERS-COV
OT  - Spike protein
OT  - T-and B-cell epitopes
OT  - Vaccine design
COIS- The authors declare that they have no competing interests.
EDAT- 2019/11/11 06:00
MHDA- 2020/09/12 06:00
PMCR- 2019/11/08
CRDT- 2019/11/10 06:00
PHST- 2019/08/15 00:00 [received]
PHST- 2019/10/26 00:00 [accepted]
PHST- 2019/11/10 06:00 [entrez]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/09/12 06:00 [medline]
PHST- 2019/11/08 00:00 [pmc-release]
AID - 10.1186/s12967-019-2116-8 [pii]
AID - 2116 [pii]
AID - 10.1186/s12967-019-2116-8 [doi]
PST - epublish
SO  - J Transl Med. 2019 Nov 8;17(1):362. doi: 10.1186/s12967-019-2116-8.