PMID- 31703754
OWN - NLM
STAT- MEDLINE
DCOM- 20200318
LR  - 20200318
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 17
IP  - 10
DP  - 2019 Oct
TI  - DNA barcoding and rapid identification of the precious herb Herba Anoectochili.
PG  - 738-745
LID - S1875-5364(19)30090-1 [pii]
LID - 10.1016/S1875-5364(19)30090-1 [doi]
AB  - Herba Anoectochili is a commonly used medicinal material. However, its 
      adulteration is a serious concern. Due to the similar morphological 
      characteristics of Herba Anoectochili and its adulterants, traditional 
      identification techniques often fail to distinguish between them accurately, 
      which is not conducive to the circulation management and safety of the medicinal 
      materials. To improve the distinction between Herba Anoectochili and its 
      adulterants accurately, this study identified 41 Herba Anoectochili and its 
      adulterant samples based on the ITS2 sequence. Sequence characteristics, Basic 
      Local Alignment Search Tool (BLAST) application, genetic distance, construction 
      of phylogenetic tree, secondary structure prediction, and other methods showed 
      the ITS2 sequence to accurately identify Herba Anoectochili from its adulterants. 
      Furthermore, in this study, we designed a specific primer, based on the ITS2 
      sequence, and established a real-time PCR detection system for the rapid, 
      sensitive, and specific identification of the original plant of Herba 
      Anoectochili. Compared to DNA barcoding technology, this method has shorter 
      detection time, stronger specificity, and higher sensitivity, which lays the 
      foundation for the rapid identification of Herba Anoectochili.
CI  - Copyright (c) 2019 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Hu, Shuai-Jun
AU  - Hu SJ
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan 430070, China.
FAU - Hu, Hao-Yu
AU  - Hu HY
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
FAU - Gao, Han
AU  - Gao H
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan 430070, China.
FAU - Liu, Xia
AU  - Liu X
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan 430070, China. Electronic address: lrx1125@126.com.
FAU - Chen, Shi-Lin
AU  - Chen SL
AD  - School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of 
      Technology, Wuhan 430070, China; Institute of Chinese Materia Medica, China 
      Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: 
      slchen@icmm.ac.cn.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - DNA Barcoding, Taxonomic
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal Spacer/genetics
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Phylogeny
MH  - Plants, Medicinal/classification/*genetics
MH  - Quality Control
OTO - NOTNLM
OT  - DNA barcode
OT  - Herba Anoectochili
OT  - ITS2
OT  - Rapid identification
OT  - Real-time PCR
EDAT- 2019/11/11 06:00
MHDA- 2020/03/19 06:00
CRDT- 2019/11/10 06:00
PHST- 2019/08/26 00:00 [received]
PHST- 2019/11/10 06:00 [entrez]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/03/19 06:00 [medline]
AID - S1875-5364(19)30090-1 [pii]
AID - 10.1016/S1875-5364(19)30090-1 [doi]
PST - ppublish
SO  - Chin J Nat Med. 2019 Oct;17(10):738-745. doi: 10.1016/S1875-5364(19)30090-1.