PMID- 31703755
OWN - NLM
STAT- MEDLINE
DCOM- 20200318
LR  - 20200318
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 17
IP  - 10
DP  - 2019 Oct
TI  - YQWY decoction reverses cardiac hypertrophy induced by TAC through inhibiting 
      GATA4 phosphorylation and MAPKs.
PG  - 746-755
LID - S1875-5364(19)30091-3 [pii]
LID - 10.1016/S1875-5364(19)30091-3 [doi]
AB  - To investigate the effect of Yiqi Wenyang (YQWY) decoction on reversing cardiac 
      hypertrophy induced by the transverse aortic constriction (TAC). Wistar rats aged 
      7-8 weeks were subjected to TAC surgery and then randomly divided into 4 groups 
      (n = 5/group): Sham group, TAC group, low-dose group and high dose group. After 
      16-week intragastric administration of YQWY decoction, the effect of YQWY 
      decoction on alleviating cardiomyocyte hypertrophy was examined by transthoracic 
      echocardiography (TTE), hematoxylin/eosin (HE), wheat germ agglutinin (WGA) 
      staining, enzyme linked immunosorbent assay (ELISA), Western blot (WB), 
      immunohistochemistry (IHC) and immunofluorescence (IF), respectively. The results 
      showed significant differences in left ventricle volume-diastole/systole (LV Vol 
      d/s), N-terminal pro-B-type brain natriuretic peptide (NT-proBNP) (P < 0.01), 
      Ejection Fraction (EF), LV mass and fractional shortening (FS) (P < 0.05) between 
      YQWY-treated group and TAC group. HE and WGA staining showed that treatment with 
      YQWY decoction dramatically prevented TAC-induced cardiomycyte hypertrophy. 
      Moreover, the results of WB, IHC and IF indicated that administration of YQWY 
      could suppress the expressions of cardiac hypertrophic markers, which included 
      the atrial natriuretic peptide (ANP), BNP and myosin heavy chain 7 (MYH7) (P < 
      0.05) and inhibit phosphorylation of GATA binding protein 4 (P-GATA4) (P < 0.05), 
      phosphorylation of extracellular signal-regulated kinase (P-ERK) (P < 0.05), 
      phosphorylation of P38 mitogen activated protein kinase (P-P38) (P < 0.05) and 
      phosphorylation of c-Jun N-terminal kinase (P-JNK) (P < 0.05). Thus, we concluded 
      that YQWY decoction suppressed cardiomyocyte hypertrophy and reversed the 
      impaired heart function, and the curative effects of YQWY decoction were 
      associated with the decreased phosphorylation of GATA4 and mitogen activated 
      protein kinases (MAPKs), as well as the reduced expression of the downstream 
      targets of GATA4, including ANP, BNP, and MYH7.
CI  - Copyright (c) 2019 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Huang, Jing-Jing
AU  - Huang JJ
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Xie, Yong
AU  - Xie Y
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Li, He
AU  - Li H
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Zhang, Xiao-Xiao
AU  - Zhang XX
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Huang, Qing
AU  - Huang Q
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Zhu, Yao
AU  - Zhu Y
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China.
FAU - Gu, Ping
AU  - Gu P
AD  - Department of Endocrinology, Jinling Hospital, School of Medicine, Nanjing 
      University, Nanjing 21002, China. Electronic address: ping-19762000@163.com.
FAU - Jiang, Wei-Min
AU  - Jiang WM
AD  - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu 
      Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China. 
      Electronic address: jwm0410@njucm.edu.cn.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (GATA4 Transcription Factor)
RN  - 0 (Gata4 protein, rat)
RN  - 0 (MYH7 protein, rat)
RN  - 0 (Peptide Fragments)
RN  - 0 (pro-brain natriuretic peptide (1-76))
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Aorta/surgery
MH  - Cardiomegaly/*drug therapy/genetics/*metabolism
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - GATA4 Transcription Factor/genetics/*metabolism
MH  - Humans
MH  - Male
MH  - Mitogen-Activated Protein Kinases/genetics/*metabolism
MH  - Myosin Heavy Chains/genetics/metabolism
MH  - Natriuretic Peptide, Brain/genetics/metabolism
MH  - Peptide Fragments/genetics/metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Cardiac hypertrophy
OT  - GATA4 phosphorylation
OT  - MAPKs
OT  - YQWY decoction
EDAT- 2019/11/11 06:00
MHDA- 2020/03/19 06:00
CRDT- 2019/11/10 06:00
PHST- 2019/05/17 00:00 [received]
PHST- 2019/11/10 06:00 [entrez]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/03/19 06:00 [medline]
AID - S1875-5364(19)30091-3 [pii]
AID - 10.1016/S1875-5364(19)30091-3 [doi]
PST - ppublish
SO  - Chin J Nat Med. 2019 Oct;17(10):746-755. doi: 10.1016/S1875-5364(19)30091-3.