PMID- 31704204
OWN - NLM
STAT- MEDLINE
DCOM- 20200421
LR  - 20200421
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 95
DP  - 2019 Dec
TI  - Multivesicular bodies containing exosomes in immune-related cells of the 
      intestine in zebrafish (Danio rerio): Ultrastructural evidence.
PG  - 644-649
LID - S1050-4648(19)31007-1 [pii]
LID - 10.1016/j.fsi.2019.10.044 [doi]
AB  - Exosomes are secreted from various cells by multivesicular bodies (MVBs) that 
      fuse with the plasma membrane and are involved in the intestinal immune response 
      to maintain intestinal homeostasis. Here, we demonstrate the ultrastructural 
      characteristics of MVBs and their exosomes in immune-related cells of the 
      zebrafish intestine, including goblet cells (GCs), mitochondria-rich cells 
      (MRCs), high endothelial cells (HECs) and lymphocytes. In GCs, MVBs with a low 
      electron density were present under the nucleus. MVBs with exosomes were observed 
      among mucin granules. "Heterogeneous" MVBs were identified within the cytoplasm 
      around mucin granules. MRCs were observed in the intestinal mucosa epithelium, 
      including "open-type" MRCs and "close-type" MRCs. Typical MVBs were identified in 
      these MRCs. MVBs with a variety of exosomes were observed in the HECs of the 
      capillary located in the lamina propria (LP). The HEC basement membrane budded 
      outward to LP cells to form a plurality of basal blebs, later containing a large 
      number of exosomes. MVBs also existed in the LP lymphocytes. A schematic diagram 
      of the ultrastructural distribution of MVBs and their exosomes in the intestinal 
      mucosal immune-related cells was created. Our findings provide cytological 
      evidence for the source and ultrastructural distribution of exosomes within the 
      different intestine cells of zebrafish. Component analysis and immunological 
      functions of exosomes require future study.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Bai, Xuebing
AU  - Bai X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Shi, Yonghong
AU  - Shi Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Tarique, Imran
AU  - Tarique I
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Vistro, Waseem Ali
AU  - Vistro WA
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Huang, Yufei
AU  - Huang Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Chen, Hong
AU  - Chen H
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Haseeb, Abdul
AU  - Haseeb A
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Gandahi, Noor Samad
AU  - Gandahi NS
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Yang, Ping
AU  - Yang P
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China.
FAU - Chen, Qiusheng
AU  - Chen Q
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province, 210095, China. Electronic address: chenqsh305@njau.edu.cn.
FAU - Lin, Jinxing
AU  - Lin J
AD  - Shanghai Laboratory Animal Research Center, Shanghai, 201203, China. Electronic 
      address: linjinxing83@163.com.
LA  - eng
PT  - Journal Article
DEP - 20191105
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Exosomes/*immunology/*ultrastructure
MH  - Female
MH  - Intestines/*cytology/*immunology
MH  - Microscopy, Electron, Transmission
MH  - Multivesicular Bodies/*immunology/ultrastructure
MH  - Zebrafish/*immunology
OTO - NOTNLM
OT  - Exosome
OT  - Immune-related cells
OT  - Multivesicular body
OT  - Zebrafish intestine
EDAT- 2019/11/11 06:00
MHDA- 2020/04/22 06:00
CRDT- 2019/11/10 06:00
PHST- 2019/07/24 00:00 [received]
PHST- 2019/09/28 00:00 [revised]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/04/22 06:00 [medline]
PHST- 2019/11/10 06:00 [entrez]
AID - S1050-4648(19)31007-1 [pii]
AID - 10.1016/j.fsi.2019.10.044 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Dec;95:644-649. doi: 10.1016/j.fsi.2019.10.044. Epub 
      2019 Nov 5.