PMID- 31704621
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1132
DP  - 2019 Nov 15
TI  - Application of response surface modeling and chemometrics methods for the 
      determination of Atenolol, Metoprolol and Propranolol in blood sample using 
      dispersive liquid-liquid microextraction combined with HPLC-DAD.
PG  - 121823
LID - S1570-0232(19)31104-3 [pii]
LID - 10.1016/j.jchromb.2019.121823 [doi]
AB  - A novel dispersive liquid-liquid microextraction (DLLME) method using ionic 
      liquids (ILs) followed by high-performance liquid chromatography-diode array 
      detector (HPLC-DAD) has been devised to specify Atenolol, Atenolol, Metoprolol 
      and Propranolol in blood real samples. Fourteen effective parameters in DLLME 
      process, including pH of aqueous sample, volume of the dispersion and extraction 
      solvents and ionic strength of donor phase, etc.; were screened using fractional 
      factorial screening methodology (FFSM) based on Placket-Burman design (PBD) and 
      subsequently were optimized by response surface methodology using central 
      composite design (CCD). A mixture of IL (1-butyl-3-methyl imidazolium hexa fluoro 
      phosphate) and disperser solvent (methanol) was quickly injected into the sample 
      solution leading to the formation of the semi cloudy solution. Afterwards, 
      HPLC-DAD was applied to examine the sedimented IL drop. The detection limits 
      (LOD) for all analytes ranges were 0.00268-0.00300 microg L(-1). The relative 
      standard deviations (RSDs) for seven experiments were between 3.832% and 4.432% 
      for three target analytes. The proposed method illustrated wide dynamic linear 
      range (DLR, 0.009-1 microg L(-1)), desirable linearity (R(2)  approximately  0.997), high 
      enrichment factors (EF, 313-330) and good relative recoveries (RR, 96-104%). 
      Clear separation and desirable chromatogram was quickly reached without the 
      intervention of the matrix. Besides, a comparison of this method with previous 
      methods indicated that the suggested method is a reproducible, quick and 
      dependable sample pretreatment technique for extraction and determination of 
      pharmaceuticals in blood sample.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Raoufi, Arastou
AU  - Raoufi A
AD  - Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
FAU - Ebrahimi, Mahmoud
AU  - Ebrahimi M
AD  - Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran. 
      Electronic address: ebrachem2007@yahoo.com.
FAU - Bozorgmehr, Mohammad Reza
AU  - Bozorgmehr MR
AD  - Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20191011
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Ionic Liquids)
RN  - 0 (Phenoxypropanolamines)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Ionic Liquids
MH  - Limit of Detection
MH  - Linear Models
MH  - Liquid Phase Microextraction/*methods
MH  - Male
MH  - Phenoxypropanolamines/*blood/chemistry/isolation & purification
MH  - Reproducibility of Results
MH  - Young Adult
OTO - NOTNLM
OT  - Atenolol
OT  - Dispersive liquid-liquid microextraction
OT  - HPLC-DAD
OT  - Ionic liquid
OT  - Metoprolol
OT  - Propranolol
EDAT- 2019/11/11 06:00
MHDA- 2020/01/23 06:00
CRDT- 2019/11/10 06:00
PHST- 2019/07/21 00:00 [received]
PHST- 2019/09/28 00:00 [revised]
PHST- 2019/10/02 00:00 [accepted]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
PHST- 2019/11/10 06:00 [entrez]
AID - S1570-0232(19)31104-3 [pii]
AID - 10.1016/j.jchromb.2019.121823 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Nov 15;1132:121823. doi: 
      10.1016/j.jchromb.2019.121823. Epub 2019 Oct 11.