PMID- 31704988
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Nov 8
TI  - Reconstruction of fish allergenicity from the content and structural traits of 
      the component beta-parvalbumin isoforms.
PG  - 16298
LID - 10.1038/s41598-019-52801-6 [doi]
LID - 16298
AB  - Most fish-allergic patients have anti-beta-parvalbumin (beta-PV) immunoglobulin E 
      (IgE), which cross-reacts among fish species with variable clinical effects. 
      Although the beta-PV load is considered a determinant for allergenicity, fish 
      species express distinct beta-PV isoforms with unknown pathogenic contributions. To 
      identify the role various parameters play in allergenicity, we have taken Gadus 
      morhua and Scomber japonicus models, determined their beta-PV isoform composition 
      and analyzed the interaction of the IgE from fish-allergic patient sera with 
      these different conformations. We found that each fish species contains a major 
      and a minor isoform, with the total PV content four times higher in Gadus morhua 
      than in Scomber japonicus. The isoforms showing the best IgE recognition 
      displayed protease-sensitive globular folds, and if forming amyloids, they were 
      not immunoreactive. Of the isoforms displaying stable globular folds, one was not 
      recognized by IgE under any of the conditions, and the other formed highly 
      immunoreactive amyloids. The results showed that Gadus morhua muscles are 
      equipped with an isoform combination and content that ensures the IgE recognition 
      of all PV folds, whereas the allergenic load of Scomber japonicus is under the 
      control of proteolysis. We conclude that the consideration of isoform properties 
      and content may improve the explanation of fish species allergenicity 
      differences.
FAU - Perez-Tavarez, Raquel
AU  - Perez-Tavarez R
AD  - Insto Quimica-Fisica "Rocasolano", Consejo Superior de Investigaciones 
      Cientificas, 28006, Madrid, Spain.
FAU - Carrera, Monica
AU  - Carrera M
AD  - Insto Investigaciones Marinas, Consejo Superior de Investigaciones Cientificas, 
      36208, Vigo, Spain.
FAU - Pedrosa, Maria
AU  - Pedrosa M
AD  - Dpto de Alergologia, Hospital Universitario La Paz, 28046, Madrid, Spain.
AD  - Insto de Investigacion Hospital Universitario La Paz (IdiPaz), 28046, Madrid, 
      Spain.
FAU - Quirce, Santiago
AU  - Quirce S
AD  - Dpto de Alergologia, Hospital Universitario La Paz, 28046, Madrid, Spain.
AD  - Insto de Investigacion Hospital Universitario La Paz (IdiPaz), 28046, Madrid, 
      Spain.
FAU - Rodriguez-Perez, Rosa
AU  - Rodriguez-Perez R
AD  - Insto de Investigacion Hospital Universitario La Paz (IdiPaz), 28046, Madrid, 
      Spain.
FAU - Gasset, Maria
AU  - Gasset M
AUID- ORCID: 0000-0001-6436-4055
AD  - Insto Quimica-Fisica "Rocasolano", Consejo Superior de Investigaciones 
      Cientificas, 28006, Madrid, Spain. maria.gasset@csic.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191108
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Allergens)
RN  - 0 (Fish Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (Protein Isoforms)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/*immunology
MH  - Amino Acid Sequence
MH  - Fish Proteins/*chemistry/*immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Muscles
MH  - Parvalbumins/*chemistry/*immunology
MH  - Protein Conformation
MH  - *Protein Isoforms
MH  - Species Specificity
MH  - Structure-Activity Relationship
PMC - PMC6841720
COIS- The authors declare no competing interests.
EDAT- 2019/11/11 06:00
MHDA- 2020/11/03 06:00
PMCR- 2019/11/08
CRDT- 2019/11/10 06:00
PHST- 2019/06/03 00:00 [received]
PHST- 2019/10/23 00:00 [accepted]
PHST- 2019/11/10 06:00 [entrez]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2019/11/08 00:00 [pmc-release]
AID - 10.1038/s41598-019-52801-6 [pii]
AID - 52801 [pii]
AID - 10.1038/s41598-019-52801-6 [doi]
PST - epublish
SO  - Sci Rep. 2019 Nov 8;9(1):16298. doi: 10.1038/s41598-019-52801-6.