PMID- 31705999
OWN - NLM
STAT- MEDLINE
DCOM- 20200605
LR  - 20200605
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 138
DP  - 2020 Jan
TI  - Enhancement of host infectivity, immunity, and protective efficacy by addition of 
      sodium bicarbonate antacid to oral vaccine formulation of live attenuated 
      Salmonella secreting Brucella antigens.
PG  - 103857
LID - S0882-4010(19)31530-X [pii]
LID - 10.1016/j.micpath.2019.103857 [doi]
AB  - In the present study, the importance of sodium bicarbonate antacid as an agent 
      for an orally delivered attenuated Salmonella strain secreting Brucella antigens 
      Cu-Zn superoxide dismutase (SodC) and outer membrane protein 19 (Omp19) as a live 
      vaccine candidate against Brucella infection was investigated. First, Brucella 
      antigens SodC and Omp19 were cloned into a prokaryotic constitutive expression 
      vector, pJHL65. Then secretion of proteins was verified after transformation into 
      an attenuated Salmonella typhimurium (ST) strain, JOL1800 (Deltalon, DeltacpxR, Deltaasd, 
      DeltarfaL), using western blot analysis. Mice were orally inoculated with 
      phosphate-buffered saline (PBS) or with a co-mixture Salmonella secreting each 
      antigens at a 1:1 ratio, each containing 1 x 10(8) CFU/mouse with and without 
      sodium bicarbonate treatment. For antacid treatment, 1.3% w/v sodium bicarbonate 
      was orally administered 30 min before and immediately after immunization with the 
      Salmonella formulation. Humoral and cell-mediated immune responses were evaluated 
      to investigate the efficacy of sodium bicarbonate in an oral formulation. The 
      results indicated that addition of sodium bicarbonate to the vaccine 
      significantly increased (P < 0.05) levels of anti-Brucella-specific systemic IgG 
      responses, lymphocyte proliferation, and CD4(+) T cell responses, indicating 
      induction of a mixed Th1-Th2 response. Immunohistochemical assays and bacterial 
      enumeration in intestinal samples also indicated that administration of sodium 
      bicarbonate enhanced colonization of Salmonella. These results indicate that 
      ingestion of the Salmonella formulation with sodium bicarbonate can enhance 
      colonization of Salmonella and induce a significant protective immune response 
      against Brucella compared with a formulation without sodium bicarbonate. Thus, 
      incorporation of sodium bicarbonate as an antacid buffer is highly recommended 
      for this oral live vaccine.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Hewawaduge, Chamith
AU  - Hewawaduge C
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, 
      Iksan, Republic of Korea.
FAU - Senevirathne, Amal
AU  - Senevirathne A
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, 
      Iksan, Republic of Korea.
FAU - Lee, John Hwa
AU  - Lee JH
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, 
      Iksan, Republic of Korea. Electronic address: johnhlee@jbnu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20191106
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Brucella Vaccine)
RN  - 0 (Vaccines, Attenuated)
RN  - 8MDF5V39QO (Sodium Bicarbonate)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Bacterial Vaccines/administration & dosage/biosynthesis/chemistry
MH  - *Brucella Vaccine/administration & dosage/biosynthesis/chemistry
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - Intestines/immunology/microbiology
MH  - Mice
MH  - Microorganisms, Genetically-Modified
MH  - Salmonella typhimurium/genetics/immunology
MH  - *Sodium Bicarbonate/administration & dosage
MH  - Transformation, Bacterial
MH  - Vaccination/methods
MH  - *Vaccines, Attenuated/administration & dosage/biosynthesis/chemistry
OTO - NOTNLM
OT  - Antacid agent
OT  - Brucella
OT  - Humoral and cell-mediated immunity
OT  - Oral vaccination
OT  - Salmonella typhimurium
COIS- Declaration of competing interest The authors declare that they have no competing 
      interests.
EDAT- 2019/11/11 06:00
MHDA- 2020/06/06 06:00
CRDT- 2019/11/10 06:00
PHST- 2019/08/28 00:00 [received]
PHST- 2019/11/05 00:00 [revised]
PHST- 2019/11/05 00:00 [accepted]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/06/06 06:00 [medline]
PHST- 2019/11/10 06:00 [entrez]
AID - S0882-4010(19)31530-X [pii]
AID - 10.1016/j.micpath.2019.103857 [doi]
PST - ppublish
SO  - Microb Pathog. 2020 Jan;138:103857. doi: 10.1016/j.micpath.2019.103857. Epub 2019 
      Nov 6.