PMID- 31707626
OWN - NLM
STAT- MEDLINE
DCOM- 20200716
LR  - 20200716
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 24
IP  - 8
DP  - 2020 Aug
TI  - Guanylate-binding protein 6 is a novel biomarker for tumorigenesis and prognosis 
      in tongue squamous cell carcinoma.
PG  - 2673-2682
LID - 10.1007/s00784-019-03129-y [doi]
AB  - OBJECTIVES: Guanylate-binding protein 6 (GBP6) is a member of the 
      guanylate-binding protein family, and its role in cancer has not yet been 
      reported. We aimed to investigate the clinical significance of GBP6 in oral 
      squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Next-generation sequencing 
      was applied for analyzing differential gene expression profiling between 
      corresponding tumor adjacent normal (CTAN) and tumor tissue from two paired OSCC 
      patients. Real-time PCRs (RT-PCRs) were used to investigate the gene expression 
      level of GBP6 of CTAN and tumor tissue samples from 14 TSCC patients. 
      Immunohistochemistry was used to investigate the protein expression level of GBP6 
      in tumor tissues and paired CTAN tissues from 488 OSCC patients, including 183 
      buccal mucosa squamous cell carcinoma (BMSCC), 245 tongue squamous cell carcinoma 
      (TSCC), and 60 lip squamous cell carcinoma (LSCC) patients. RESULTS: Compared 
      with CTAN tissues of OSCC patients, GBP6 is identified as a downregulated gene 
      using the NGS platform, which was confirmed in 14 OSCC patients by RT-PCR. 
      Moreover, protein expression level of GBP6 in tumor tissues was lower than that 
      in CTAN tissues and the low GBP6 expression was correlated with poor cell 
      differentiation/lymph node metastasis in TSCC patients. In addition, TSCC 
      patients with low expression levels of GBP6 had poor disease-specific survival 
      rate. CONCLUSION: The low expression of GBP6 was associated with tumorigenesis 
      and poor prognosis in OSCC patients, especially in TSCC patients. CLINICAL 
      RELEVANCE: GBP6 may serve as a novel favorable diagnostic and prognostic 
      biomarker in TSCC patients.
FAU - Liu, Pei-Feng
AU  - Liu PF
AUID- ORCID: 0000-0002-7849-8940
AD  - Department of Biomedical Science and Environmental Biology, Kaohsiung Medical 
      University, Kaohsiung, Taiwan. pfliu@kmu.edu.tw.
AD  - Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 
      Taiwan. pfliu@kmu.edu.tw.
FAU - Chen, Hung-Chih
AU  - Chen HC
AD  - Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, 
      Taiwan.
AD  - Department of Dental Technology, Shu-Zen Junior College of Medicine and 
      Management, Kaohsiung, Taiwan.
FAU - Shu, Chih-Wen
AU  - Shu CW
AD  - Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 
      Taiwan.
AD  - School of Medicine for International Students, I-Shou University, Kaohsiung, 
      Taiwan.
FAU - Sie, Huei-Cin
AU  - Sie HC
AD  - Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General 
      Hospital, Kaohsiung, Taiwan.
FAU - Lee, Cheng-Hsin
AU  - Lee CH
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung, Taiwan.
FAU - Liou, Huei-Han
AU  - Liou HH
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung, Taiwan.
FAU - Cheng, Jiin-Tsuey
AU  - Cheng JT
AD  - Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, 
      Taiwan.
FAU - Tsai, Kuo-Wang
AU  - Tsai KW
AD  - Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 
      Taiwan.
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung, Taiwan.
FAU - Ger, Luo-Ping
AU  - Ger LP
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung, Taiwan.
LA  - eng
GR  - VGHKS107-174, VGHKS108-178/Kaohsiung Veterans General Hospital/
GR  - MOST 106-2320-B-075B-002-MY2 and MOST 108-2320-B-075B-003/The Ministry of Science 
      and Technology/
GR  - CI-107-13/Yen Tjing Ling Medical Foundation/
PT  - Journal Article
DEP - 20191109
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - 0 (Biomarkers, Tumor)
MH  - Biomarkers, Tumor
MH  - Carcinogenesis
MH  - Cell Transformation, Neoplastic
MH  - Humans
MH  - Prognosis
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - *Tongue Neoplasms
OTO - NOTNLM
OT  - Guanylate-binding protein 6
OT  - Oral squamous cell carcinoma
OT  - Prognosis
OT  - Tumorigenesis
EDAT- 2019/11/11 06:00
MHDA- 2020/07/17 06:00
CRDT- 2019/11/11 06:00
PHST- 2019/08/14 00:00 [received]
PHST- 2019/10/16 00:00 [accepted]
PHST- 2019/11/11 06:00 [pubmed]
PHST- 2020/07/17 06:00 [medline]
PHST- 2019/11/11 06:00 [entrez]
AID - 10.1007/s00784-019-03129-y [pii]
AID - 10.1007/s00784-019-03129-y [doi]
PST - ppublish
SO  - Clin Oral Investig. 2020 Aug;24(8):2673-2682. doi: 10.1007/s00784-019-03129-y. 
      Epub 2019 Nov 9.