PMID- 31709631
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 121
IP  - 2
DP  - 2020 Feb
TI  - Ameliorative effect of combined low dose of Pioglitazone and omega-3 on 
      spermatogenesis and steroidogenesis in diabetic rats.
PG  - 1524-1540
LID - 10.1002/jcb.29388 [doi]
AB  - BACKGROUND: Chronic hyperglycemia is linked to either subfertility or infertility 
      among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs 
      that are selective peroxisome proliferator-activated receptor (PPAR-gamma agonists 
      used for treating type 2 diabetes mellitus (T2DM). AIM: This study aims to 
      explore the possible effect of low Pioglitazone dose and omega (omega-3) on rat male 
      reproductive function. Furthermore, we evaluated the add-on effect of combined 
      use of low Pioglitazone dose of and omega-3 on reproductive functions in adult male 
      T2DM rats. METHODS: Fifty adult male rats were included and subdivided into 
      control and four test subgroups. T2DM was induced in test groups and subdivided 
      into non-treated T2DM, omega-3 treated, 0.6 mg/kg Pioglitazone treated, and combined 
      treated group (orally by gavage). Following 16 weeks, final body weight, 
      testicular weight, fasting plasma glucose, and serum testosterone levels were 
      measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) 
      concentrations, superoxide dismutase (SOD) activity, immunohistochemistry 
      staining for apoptosis marker B-cell lymphoma protein 2 (Bcl-2), proliferation 
      marker as proliferating cell nuclear antigen (PCNA), estrogen receptor alpha (ERalpha), 
      androgen receptor (AR) were determined. Caspase-3, nuclear factor-kappa B 
      (NF-kB), glucose transporter 3 (GLUT3), 17beta-hydroxysteroid dehydrogenases 
      (17beta-HSD) PPARgamma, and PPARalpha genes expression were analyzed by real-time polymerase 
      chain reaction (RT-PCR). RESULTS: Our findings revealed that treatment with low 
      dose of Pioglitazone or omega-3 significantly lowered fasting plasma glucose and MDA 
      levels, ameliorated diabetes effects on histological damage, improved antioxidant 
      activity (SOD), significantly improved anti-apoptosis BCL-2 and proliferation 
      (PCNA), remarkably elevated ERalpha, AR, 17beta-HSD PPARgamma, and PPARalpha expression with 
      significant reduction in caspase-3, NF-kB genes expression and improved semen 
      quality as well. Combined use of low dose of and omega-3 has better effects on all 
      measured parameters. CONCLUSION: Small Pioglitazone dose and omega-3 possess 
      beneficial effects on spermatogenic and steroidogenic functions in adult diabetic 
      rat; while combined use of both has an add-on effect.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Hasan, Mai M
AU  - Hasan MM
AD  - Department of Medical Physiology, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
FAU - El-Shal, Amal S
AU  - El-Shal AS
AUID- ORCID: 0000-0003-2167-8841
AD  - Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
FAU - Mackawy, Amal M H
AU  - Mackawy AMH
AD  - Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
AD  - Department of Medical Lab, Applied Medical Science, Qassim University, Qassim, 
      KSA.
FAU - Ibrahim, Ebtesam M
AU  - Ibrahim EM
AD  - Department of Medical Physiology, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
FAU - Abdelghany, Eman M M A
AU  - Abdelghany EMMA
AD  - Department with Anatomy and Embryology, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
FAU - Saeed, Abeer A
AU  - Saeed AA
AD  - Department of Medical Physiology, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
FAU - El-Gendy, Jehan
AU  - El-Gendy J
AD  - Department with Medical Pharmacology, Faculty of Medicine, Zagazig University, 
      Zagazig, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20191110
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antioxidants)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (PPAR alpha)
RN  - 3XMK78S47O (Testosterone)
RN  - X4OV71U42S (Pioglitazone)
SB  - IM
MH  - Animals
MH  - Antioxidants
MH  - Diabetes Mellitus, Experimental/*complications/pathology
MH  - Diabetes Mellitus, Type 2/*complications/pathology
MH  - Dose-Response Relationship, Drug
MH  - Drug Therapy, Combination
MH  - Fatty Acids, Omega-3/administration & dosage/*pharmacology
MH  - Hypoglycemic Agents/pharmacology
MH  - Infertility, Male/*drug therapy/etiology/metabolism/pathology
MH  - Male
MH  - PPAR alpha
MH  - Pioglitazone/administration & dosage/*pharmacology
MH  - Rats
MH  - Semen Analysis
MH  - *Spermatogenesis
MH  - Testis/drug effects/metabolism/pathology
MH  - Testosterone/*metabolism
OTO - NOTNLM
OT  - PPAR
OT  - Pioglitazone
OT  - gene expression
OT  - hyperglycemia
OT  - omega-3
OT  - rat male fertility
OT  - real-time polymerase chain reaction
EDAT- 2019/11/12 06:00
MHDA- 2021/05/11 06:00
CRDT- 2019/11/12 06:00
PHST- 2018/12/30 00:00 [received]
PHST- 2019/08/20 00:00 [accepted]
PHST- 2019/11/12 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2019/11/12 06:00 [entrez]
AID - 10.1002/jcb.29388 [doi]
PST - ppublish
SO  - J Cell Biochem. 2020 Feb;121(2):1524-1540. doi: 10.1002/jcb.29388. Epub 2019 Nov 
      10.