PMID- 31713587
OWN - NLM
STAT- MEDLINE
DCOM- 20201016
LR  - 20231213
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 12
DP  - 2019 Dec 20
TI  - SP1-DLEU1-miR-4429 feedback loop promotes cell proliferative and anti-apoptotic 
      abilities in human glioblastoma.
LID - 10.1042/BSR20190994 [doi]
LID - BSR20190994
AB  - Mounting studies have revealed that long non-coding RNA (lncRNA) deleted in 
      lymphocytic leukemia 1 (DLEU1) positively regulated the initiation and 
      development of various human malignant tumors. Nevertheless, the function and 
      mechanism of DLEU1 in human glioblastoma multiforme (GBM) remain elusive and 
      ill-defined. The current study was designed to highlight the functional role and 
      disclose the underlying molecular mechanism by which DLEU1 regulated GBM 
      development. We found that DLEU1 was up-regulated in GBM and DLEU1 knockdown 
      significantly inhibited GBM cell proliferation and induced apoptosis. As 
      predicted by bioinformatics analysis and validated in mechanistic assays, SP1 
      could bind to the promoter region of DLEU1 to activate DLEU1 transcription. 
      Additionally, miR-4429 was verified as a target gene of DLEU1 and negatively 
      modulated by DLEU1. More importantly, miR-4429 overexpression repressed the mRNA 
      and protein levels of SP1 via binding to the 3'UTR of SP1. Overexpression of SP1 
      or miR-4429 inhibitor could partly abolish the effect of DLEU1 knockdown on cell 
      viability and apoptosis in GBM. Accordingly, our experimental data revealed that 
      SP1-DLEU1-miR-4429 formed a feedback loop to promote GBM development, providing a 
      new evidence for the role of DLEU1 in GBM.
CI  - (c) 2019 The Author(s).
FAU - Liu, Xiaolei
AU  - Liu X
AD  - Department of Neurosurgery, Xianyang Hospital of Yan'an University, Xianyang 
      City, Shaanxi Province 712000, P.R. China.
FAU - Chen, Ruwei
AU  - Chen R
AD  - Department of Neurosurgery, Binzhou People's Hospital, Shandong Province 256610, 
      P.R. China.
FAU - Liu, Lijun
AU  - Liu L
AD  - Department of Neurosurgery, Xiangyang No. 1 People's Hospital Affiliated to Hubei 
      University of Medicine, Xiangyang 441000, Hubei, P.R. China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (DLEU1 lncRNA, human)
RN  - 0 (MIRN4429 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (SP1 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Apoptosis/genetics
MH  - Carcinogenesis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Glioblastoma/*genetics/pathology
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - RNA, Long Noncoding
MH  - Sp1 Transcription Factor/*genetics
MH  - Tumor Suppressor Proteins/*genetics
PMC - PMC6900472
OTO - NOTNLM
OT  - DLEU1
OT  - SP1
OT  - feedback loop
OT  - glioblastoma
OT  - miR-4429
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2019/11/13 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/12/06
CRDT- 2019/11/13 06:00
PHST- 2019/04/12 00:00 [received]
PHST- 2019/10/29 00:00 [revised]
PHST- 2019/11/11 00:00 [accepted]
PHST- 2019/11/13 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/11/13 06:00 [entrez]
PHST- 2019/12/06 00:00 [pmc-release]
AID - 221115 [pii]
AID - BSR20190994 [pii]
AID - 10.1042/BSR20190994 [doi]
PST - ppublish
SO  - Biosci Rep. 2019 Dec 20;39(12):BSR20190994. doi: 10.1042/BSR20190994.