PMID- 31715290 OWN - NLM STAT- MEDLINE DCOM- 20201210 LR - 20201214 IS - 1872-7972 (Electronic) IS - 0304-3940 (Linking) VI - 715 DP - 2020 Jan 10 TI - Inhaled molecular hydrogen attenuates intense acute exercise-induced hippocampal inflammation in sedentary rats. PG - 134577 LID - S0304-3940(19)30680-9 [pii] LID - 10.1016/j.neulet.2019.134577 [doi] AB - Physical exercise-induced inflammation may be beneficial when exercise is regular but it may be harmful when exercise is intense and performed by unaccustomed individuals/rats. Molecular hydrogen (H(2)) has recently emerged as a powerful anti-inflammatory, antioxidant and anti-apoptotic molecule in a number of pathological conditions, but little is known about its putative role under physiological conditions such as physical exercise. Therefore, we tested the hypothesis that H(2) decreases intense acute exercise-induced inflammation in the hippocampus, since it is a brain region particularly susceptible to inflammation. Moreover, we also assessed hippocampus oxidative status. Rats ran on a sealed treadmill inhaling either the H(2) (2% H(2), 21% O(2), balanced with N(2)) or the control gas (0% H(2), 21% O(2), balanced with N(2)) and hippocampal samples were collected immediately or 3 h after exercise. We measured hippocampal levels of cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-6 and IL-10] and oxidative markers [superoxide dismutase (SOD), thiobarbituric acid reactive species (TBARS) and nitrite/nitrate (NOx)]. Exercise increased TNF-alpha, IL-6 and IL-10 immediately after the session, whereas no change in IL-1beta levels was observed. Conversely, exercise did not cause any change in SOD activity, TBARS and NOx levels. H(2) inhibited the exercise-induced surges in TNF-alpha and IL-6, and potentiated the IL-10 surge, immediately after the exercise. Moreover, no change in IL1-beta levels of rats inhaling H(2) was observed. Regarding the oxidative stress markers, H(2) failed to cause any change in SOD activity, TBARS and NOx levels. No significant change was observed in any of the assessed parameters 3 h after the exercise bout. These data are consistent with the notion that H(2) acts as a powerful anti-inflammatory agent not only down-modulating pro-inflammatory cytokines (TNF-alpha and IL-6) but also upregulating an anti-inflammatory cytokine (IL-10) production without affecting the local oxidative stress status. These data indicate that H(2) effectively decreases exercise-induced inflammation in the hippocampus, despite the fact that this region is particularly prone to inflammatory insults. CI - Copyright (c) 2019. Published by Elsevier B.V. FAU - Nogueira, Jonatas E AU - Nogueira JE AD - Postgraduate Program in Rehabilitation and Functional Performance, University of Sao Paulo, Ribeirao Preto, SP, Brazil; School of Physical Education and Sports of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. FAU - de Deus, Junia L AU - de Deus JL AD - Department of Basic and Oral Biology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. FAU - Amorim, Mateus R AU - Amorim MR AD - Department of Basic and Oral Biology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. FAU - Batalhao, Marcelo E AU - Batalhao ME AD - Department of General and Specialized Nursing, School of Nursing of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. FAU - Leao, Ricardo M AU - Leao RM AD - Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. FAU - Carnio, Evelin C AU - Carnio EC AD - Department of General and Specialized Nursing, School of Nursing of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil; Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. FAU - Branco, Luiz G S AU - Branco LGS AD - Postgraduate Program in Rehabilitation and Functional Performance, University of Sao Paulo, Ribeirao Preto, SP, Brazil; Department of Basic and Oral Biology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil; Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: branco@forp.usp.br. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191109 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Inflammation Mediators) RN - 7YNJ3PO35Z (Hydrogen) SB - IM MH - Administration, Inhalation MH - Animals MH - Anti-Inflammatory Agents/*administration & dosage MH - Hippocampus/drug effects/*metabolism MH - Hydrogen/*administration & dosage MH - Inflammation/etiology/metabolism/prevention & control MH - Inflammation Mediators/antagonists & inhibitors/*metabolism MH - Male MH - Physical Conditioning, Animal/*adverse effects/trends MH - Random Allocation MH - Rats MH - Rats, Wistar MH - *Sedentary Behavior OTO - NOTNLM OT - Physical exercise OT - ROS OT - cytokines OT - hippocampus EDAT- 2019/11/13 06:00 MHDA- 2020/12/15 06:00 CRDT- 2019/11/13 06:00 PHST- 2019/07/05 00:00 [received] PHST- 2019/09/24 00:00 [revised] PHST- 2019/10/21 00:00 [accepted] PHST- 2019/11/13 06:00 [pubmed] PHST- 2020/12/15 06:00 [medline] PHST- 2019/11/13 06:00 [entrez] AID - S0304-3940(19)30680-9 [pii] AID - 10.1016/j.neulet.2019.134577 [doi] PST - ppublish SO - Neurosci Lett. 2020 Jan 10;715:134577. doi: 10.1016/j.neulet.2019.134577. Epub 2019 Nov 9.