PMID- 31717606
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 11
IP  - 11
DP  - 2019 Nov 9
TI  - DUSP10 Is a Regulator of YAP1 Activity Promoting Cell Proliferation and 
      Colorectal Cancer Progression.
LID - 10.3390/cancers11111767 [doi]
LID - 1767
AB  - Cell contact inhibition (CCI) is deregulated in cancer. Colorectal cancer (CRC) 
      is the third most commonly diagnosed cancer worldwide. We found that 
      dual-specificity phosphatase 10 (DUSP10) is involved in CRC. DUSP10 
      overexpression increased the growth of CRC cell lines and mouse xenografts, while 
      the opposite phenotype was observed by DUSP10 silencing. High cell density (HD) 
      induced DUSP10 expression in CRC cell lines, particularly within the nucleus. 
      Yes-associated protein 1 (YAP1) is activated by dephosphorylation, controlling 
      organ growth and CCI, both processes being deregulated in CRC. Expression levels 
      and localization of DUSP10 matched with YAP1 levels in CRC cell lines. DUSP10 and 
      YAP1 co-immunoprecipitated and their interaction was dependent on YAP1 Ser397. 
      The existence of DUSP10 and YAP1 pathway in vivo was confirmed by using a 
      transgenic Drosophila model. Finally, in CRC patients' samples, high levels of 
      nuclear DUSP10 correlated with nuclear YAP1 in epithelial tumor tissue. Strong 
      nuclear DUSP10 staining also correlated with high tumor stage and poor survival. 
      Overall, these findings describe a DUSP10-YAP1 molecular link in CRC cell lines 
      promoting cell growth in HD. We present evidence suggesting a pro-tumorigenic 
      role of nuclear DUSP10 expression in CRC patients.
FAU - Jimenez-Martinez, Marta
AU  - Jimenez-Martinez M
AD  - Department of Cell Biology and Immunology, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
AD  - Department of Molecular Biology, Universidad Autonoma de Madrid (UAM), 28049 
      Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria de La Princesa (IIS-P), 28006 Madrid, Spain.
FAU - Ostale, Cristina M
AU  - Ostale CM
AD  - Department of Development and Regeneration, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
FAU - van der Burg, Lennart R
AU  - van der Burg LR
AD  - Department of Gastroenterology-Hepatology, Leiden University Medical Center, 
      2333ZA Leiden, The Netherlands.
FAU - Galan-Martinez, Javier
AU  - Galan-Martinez J
AD  - Department of Cell Biology and Immunology, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
AD  - Department of Molecular Biology, Universidad Autonoma de Madrid (UAM), 28049 
      Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria de La Princesa (IIS-P), 28006 Madrid, Spain.
FAU - Hardwick, James C H
AU  - Hardwick JCH
AD  - Department of Gastroenterology-Hepatology, Leiden University Medical Center, 
      2333ZA Leiden, The Netherlands.
FAU - Lopez-Perez, Ricardo
AU  - Lopez-Perez R
AUID- ORCID: 0000-0001-9495-8604
AD  - Department of Cell Biology and Immunology, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
FAU - Hawinkels, Lukas J A C
AU  - Hawinkels LJAC
AUID- ORCID: 0000-0002-2274-9325
AD  - Department of Gastroenterology-Hepatology, Leiden University Medical Center, 
      2333ZA Leiden, The Netherlands.
FAU - Stamatakis, Konstantinos
AU  - Stamatakis K
AUID- ORCID: 0000-0001-9934-3502
AD  - Department of Cell Biology and Immunology, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
AD  - Department of Molecular Biology, Universidad Autonoma de Madrid (UAM), 28049 
      Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria de La Princesa (IIS-P), 28006 Madrid, Spain.
FAU - Fresno, Manuel
AU  - Fresno M
AD  - Department of Cell Biology and Immunology, Centro de Biologia Molecular "Severo 
      Ochoa" (CSIC-UAM), 28049 Madrid, Spain.
AD  - Department of Molecular Biology, Universidad Autonoma de Madrid (UAM), 28049 
      Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria de La Princesa (IIS-P), 28006 Madrid, Spain.
LA  - eng
GR  - SAF2013-42850-R/Ministerio de Ciencia e Innovacion/
GR  - SAF2016-75988-R/Ministerio de Ciencia e Innovacion/
GR  - S2017/BMD-3671/Comunidad de Madrid/
GR  - BIOMID/Fondo de investigaciones Sanitarias La Princesa/
GR  - AIO/Fundacion Cientifica Asociacion Espanola Contra el Cancer/
PT  - Journal Article
DEP - 20191109
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC6896144
OTO - NOTNLM
OT  - cancer
OT  - cell contact inhibition
OT  - cell proliferation
OT  - prognosis
OT  - protein
COIS- The authors declare no conflict of interest.
EDAT- 2019/11/14 06:00
MHDA- 2019/11/14 06:01
PMCR- 2019/11/09
CRDT- 2019/11/14 06:00
PHST- 2019/10/18 00:00 [received]
PHST- 2019/10/31 00:00 [revised]
PHST- 2019/11/05 00:00 [accepted]
PHST- 2019/11/14 06:00 [entrez]
PHST- 2019/11/14 06:00 [pubmed]
PHST- 2019/11/14 06:01 [medline]
PHST- 2019/11/09 00:00 [pmc-release]
AID - cancers11111767 [pii]
AID - cancers-11-01767 [pii]
AID - 10.3390/cancers11111767 [doi]
PST - epublish
SO  - Cancers (Basel). 2019 Nov 9;11(11):1767. doi: 10.3390/cancers11111767.