PMID- 31720043
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 19
IP  - 5
DP  - 2019 Oct
TI  - Ubiquitin E3 Ligase Pellino-1 Inhibits IL-10-mediated M2c Polarization of 
      Macrophages, Thereby Suppressing Tumor Growth.
PG  - e32
LID - 10.4110/in.2019.19.e32 [doi]
LID - e32
AB  - Pellino-1 is a ubiquitin (Ub) E3 ligase that plays a role in M1, but not M2a 
      polarization of macrophages. However, it is unknown whether Pellino-1 regulates 
      IL-10-mediated M2c polarization of macrophages. Here, we found that Pellino-1 
      attenuated tumor growth by inhibiting M2c polarization of macrophages. Upon IL-10 
      stimulation, Pellino-1-deificient bone marrow-derived macrophages (BMDMs) showed 
      higher expression of M2c markers, but not M2a, and M2b markers than wild-type 
      (WT) BMDMs, indicating that Pellino-1 inhibits M2c polarization of macrophages. 
      Pellino-1-deficient BMDMs exhibited a defect in mitochondria respiration, but 
      enhancement of glycolysis during M2c polarization. During M2c polarization of 
      macrophages, Pellino-1 increased STAT1 phosphorylation via K63-linked 
      ubiquitination of IL-1 receptor associated kinase 1 (IRAK1). Furthermore, 
      Lysm-CrePellino-1 (fl/fl) mice showed enhancement of tumor growth via regulating 
      M2c polarization of tumor-associated macrophages. These results demonstrate that 
      Pellino-1 inhibits IL-10-induced M2c macrophage polarization via K63-linked 
      ubiquitination of IRAK1 and activation of STAT1, thereby inhibiting tumor growth 
      in vivo.
CI  - Copyright (c) 2019. The Korean Association of Immunologists.
FAU - Kim, Donghyun
AU  - Kim D
AUID- ORCID: 0000-0001-7863-7384
AD  - Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul 
      National University College of Medicine, Seoul 03080, Korea.
FAU - Koh, Jaemoon
AU  - Koh J
AUID- ORCID: 0000-0002-2824-5080
AD  - Department of Pathology, Seoul National University College of Medicine, Seoul 
      03080, Korea.
FAU - Ko, Jae Sung
AU  - Ko JS
AUID- ORCID: 0000-0003-3195-5910
AD  - Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul 
      National University College of Medicine, Seoul 03080, Korea.
FAU - Kim, Hye Young
AU  - Kim HY
AUID- ORCID: 0000-0001-5978-512X
AD  - Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul 
      National University College of Medicine, Seoul 03080, Korea.
FAU - Lee, Ho
AU  - Lee H
AD  - Graduate School of Cancer Science and Policy, Research Institute, National Cancer 
      Center, Goyang 10408, Korea.
FAU - Chung, Doo Hyun
AU  - Chung DH
AUID- ORCID: 0000-0002-9948-8485
AD  - Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul 
      National University College of Medicine, Seoul 03080, Korea.
AD  - Department of Pathology, Seoul National University College of Medicine, Seoul 
      03080, Korea.
LA  - eng
PT  - Journal Article
DEP - 20191017
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC6829073
OTO - NOTNLM
OT  - E3 ligase
OT  - M2c
OT  - Macrophages
OT  - Pellino-1
OT  - Polarization
OT  - Tumors
COIS- Conflict of Interest: The authors declare no potential conflicts of interest.
EDAT- 2019/11/14 06:00
MHDA- 2019/11/14 06:01
PMCR- 2019/10/01
CRDT- 2019/11/14 06:00
PHST- 2019/08/26 00:00 [received]
PHST- 2019/10/01 00:00 [revised]
PHST- 2019/10/02 00:00 [accepted]
PHST- 2019/11/14 06:00 [entrez]
PHST- 2019/11/14 06:00 [pubmed]
PHST- 2019/11/14 06:01 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - 2019190503 [pii]
AID - 10.4110/in.2019.19.e32 [doi]
PST - epublish
SO  - Immune Netw. 2019 Oct 17;19(5):e32. doi: 10.4110/in.2019.19.e32. eCollection 2019 
      Oct.