PMID- 31721020
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20220510
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 6
DP  - 2019 Dec
TI  - 1-Deoxynojirimycin modulates glucose homeostasis by regulating the combination of 
      IR-GlUT4 and ADIPO-GLUT4 pathways in 3T3-L1 adipocytes.
PG  - 6277-6285
LID - 10.1007/s11033-019-05069-y [doi]
AB  - 1-Deoxynojirimycin (DNJ), the main alkaloid in mulberry leaves, was recognized to 
      treat patients with type 2 diabetes mellitus (T2DM). However, the regulatory 
      mechanism of DNJ on glucose homeostasis was still unclear. In the present study, 
      a safe concentration of 0.1-10 mumol/L for DNJ was incubated with mature 3T3-L1 
      adipocytes. The results demonstrated that the genes/proteins expression of 
      insulin receptor (IR), phosphatidylinositol-3-kinase (PI3K), protein kinase B 
      (AKt/PkB), and adiponectin (ADIPO) increased with the increasing of DNJ 
      concentration from 0.1-10 mumol/L. However the mRNA expression of adenosine 
      5'-monophosphate (AMP)-activated protein kinase (AMPK), glucose transporter 4 
      (GLUT4) and glucose absorption increased to the maximum at concentration of 
      5 mumol/L then decreased with further increase of DNJ concentration to 10 mumol/L. 
      Both IR and ADIPO signaling pathways simultaneously affect the glucose 
      homeostasis regulation effect of DNJ, whereas the key response target located in 
      AMPK and its effect on subsequent GLUT4 mRNA expression.
FAU - Li, Qian
AU  - Li Q
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China.
FAU - Wang, Yiguo
AU  - Wang Y
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China.
FAU - Dai, Yanli
AU  - Dai Y
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China.
FAU - Shen, Weizhi
AU  - Shen W
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China.
FAU - Liao, Sentai
AU  - Liao S
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China. 
      liaost@163.com.
FAU - Zou, Yuxiao
AU  - Zou Y
AUID- ORCID: 0000-0002-8245-3286
AD  - Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research 
      Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural 
      Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Dongguan 
      Village, Yiheng Road No. 133, Guangzhou, 510610, Guangdong, China. 
      yuxiaozou@163.com.
LA  - eng
GR  - No.2017A030310416/Guangdong Science and Technology Department/
GR  - No. 2019B020213001/Guangdong Science and Technology Department/
GR  - 2017B020207006/Science & Technology Projects of Guangdong Province/
GR  - No. 2018LM1087/Guangdong Province Introduction of Innovative R&D Team/
GR  - No.2018LM2154/Guangdong Province Introduction of Innovative R&D Team/
PT  - Journal Article
DEP - 20191112
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Adiponectin)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (SLC2A4 protein, human)
RN  - 19130-96-2 (1-Deoxynojirimycin)
RN  - EC 2.7.10.1 (Receptor, Insulin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - 1-Deoxynojirimycin/*pharmacology
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/*metabolism
MH  - Adiponectin/*metabolism
MH  - Animals
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4/*metabolism
MH  - Homeostasis/*drug effects
MH  - Mice
MH  - Models, Biological
MH  - Receptor, Insulin/*metabolism
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - 3T3-L1 adipocytes
OT  - AMPK
OT  - DNJ
OT  - GLUT4
OT  - Glucose homeostasis
EDAT- 2019/11/14 06:00
MHDA- 2020/04/16 06:00
CRDT- 2019/11/14 06:00
PHST- 2019/05/12 00:00 [received]
PHST- 2019/09/10 00:00 [accepted]
PHST- 2019/11/14 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
PHST- 2019/11/14 06:00 [entrez]
AID - 10.1007/s11033-019-05069-y [pii]
AID - 10.1007/s11033-019-05069-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Dec;46(6):6277-6285. doi: 10.1007/s11033-019-05069-y. Epub 
      2019 Nov 12.