PMID- 31721818
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 11
DP  - 2019
TI  - Identifying resurrection genes through the differentially expressed genes between 
      Selaginella tamariscina (Beauv.) spring and Selaginella moellendorffii Hieron 
      under drought stress.
PG  - e0224765
LID - 10.1371/journal.pone.0224765 [doi]
LID - e0224765
AB  - Selaginella tamariscina (Beauv.) spring, a primitive vascular resurrection plant, 
      can survive extreme drought and recover when water becomes available. To identify 
      drought-inducible genes and to clarify the molecular mechanism of drought 
      tolerance, a comparative transcriptional pattern analysis was conducted between 
      S. tamariscina and Selaginella moellendorffii Hieron (drought sensitive). 133 
      drought related genes were identified, including 72 functional genes and 61 
      regulatory genes. And several drought responsive reactions, such as antioxidant 
      activity, osmotic balance, cuticle defense and signal transduction were 
      highlighted in S. tamariscina under drought. Notably, besides peroxidase, 
      catalase and L-ascorbate oxidase genes, DEGs associated with phenylalanine 
      metabolism and polyamine catabolism could be alternative ways to enhance 
      antioxidant ability in S. tamariscina. DEGs related to soluble carbohydrate 
      metabolism, late embryogenesis abundant protein (LEA) and aquaporin protein (AQP) 
      confirmed that osmotic adjustment could resist drought during desiccation. DEGs 
      involved in xyloglucan metabolic process, pectin metabolic process and cutin 
      biosynthesis may also contribute to drought tolerance of S. tamariscina by 
      cuticle defense. Drought-responsive genes encoding protein kinases, calcium 
      sensors, transcription factors (TFs) and plant hormones also help to drought 
      resistance of S. tamariscina. The preliminary validation experiments were 
      performed and the results were consistent with our hypothetical integrated 
      regulatory network. The results of this study provide candidate resurrection 
      genes and an integrated regulatory network for further studies on the molecular 
      mechanisms of stress tolerance in S. tamariscina.
FAU - Gu, Wei
AU  - Gu W
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
AD  - Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources 
      Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Zhang, Aqin
AU  - Zhang A
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Sun, Hongmei
AU  - Sun H
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Gu, Yuchen
AU  - Gu Y
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Chao, Jianguo
AU  - Chao J
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Tian, Rong
AU  - Tian R
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Duan, Jin-Ao
AU  - Duan JA
AUID- ORCID: 0000-0001-7109-7848
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
AD  - Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources 
      Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191113
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Desiccation
MH  - *Droughts
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant/*genetics
MH  - Selaginellaceae/*genetics
MH  - Stress, Physiological/*genetics
PMC - PMC6853609
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/11/14 06:00
MHDA- 2020/03/17 06:00
PMCR- 2019/11/13
CRDT- 2019/11/14 06:00
PHST- 2019/06/23 00:00 [received]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2019/11/14 06:00 [entrez]
PHST- 2019/11/14 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2019/11/13 00:00 [pmc-release]
AID - PONE-D-19-17762 [pii]
AID - 10.1371/journal.pone.0224765 [doi]
PST - epublish
SO  - PLoS One. 2019 Nov 13;14(11):e0224765. doi: 10.1371/journal.pone.0224765. 
      eCollection 2019.