PMID- 31722974
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20240229
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 182
IP  - 1
DP  - 2020 Jan
TI  - Visualization of Protein Coding, Long Noncoding, and Nuclear RNAs by Fluorescence 
      in Situ Hybridization in Sections of Shoot Apical Meristems and Developing 
      Flowers.
PG  - 147-158
LID - 10.1104/pp.19.00980 [doi]
AB  - In addition to transcriptional regulation, gene expression is further modulated 
      through mRNA spatiotemporal distribution, by RNA movement between cells, and by 
      RNA localization within cells. Here, we have adapted RNA fluorescence in situ 
      hybridization (FISH) to explore RNA localization in Arabidopsis (Arabidopsis 
      thaliana). We show that RNA FISH on sectioned material can be applied to 
      investigate the tissue and subcellular localization of meristem and flower 
      development genes, cell cycle transcripts, and plant long noncoding RNAs. We also 
      developed double RNA FISH to dissect the coexpression of different mRNAs at the 
      shoot apex and nuclear-cytoplasmic separation of cell cycle gene transcripts in 
      dividing cells. By coupling RNA FISH with fluorescence immunocytochemistry, we 
      further demonstrate that a gene's mRNA and protein may be simultaneously 
      detected, for example revealing uniform distribution of PIN-FORMED1 (PIN1) mRNA 
      and polar localization of PIN1 protein in the same cells. Therefore, our method 
      enables the visualization of gene expression at both transcriptional and 
      translational levels with subcellular spatial resolution, opening up the 
      possibility of systematically tracking the dynamics of RNA molecules and their 
      cognate proteins in plant cells.
CI  - (c) 2020 American Society of Plant Biologists. All Rights Reserved.
FAU - Yang, Weibing
AU  - Yang W
AUID- ORCID: 0000-0002-2379-5729
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
FAU - Schuster, Christoph
AU  - Schuster C
AUID- ORCID: 0000-0002-1948-2367
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
FAU - Prunet, Nathanael
AU  - Prunet N
AUID- ORCID: 0000-0002-8939-5920
AD  - Howard Hughes Medical Institute and Division of Biology and Biological 
      Engineering, California Institute of Technology, Pasadena, California 91125.
FAU - Dong, Qingkun
AU  - Dong Q
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agrobioresources, South China Agricultural University, Guangzhou 510642, China.
FAU - Landrein, Benoit
AU  - Landrein B
AUID- ORCID: 0000-0002-2371-9996
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
FAU - Wightman, Raymond
AU  - Wightman R
AUID- ORCID: 0000-0003-1295-4875
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
FAU - Meyerowitz, Elliot M
AU  - Meyerowitz EM
AUID- ORCID: 0000-0003-4798-5153
AD  - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom 
      meyerow@caltech.edu.
AD  - Howard Hughes Medical Institute and Division of Biology and Biological 
      Engineering, California Institute of Technology, Pasadena, California 91125.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191113
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (RNA, Nuclear)
SB  - IM
MH  - Arabidopsis/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Flowers/genetics/*metabolism
MH  - Gene Expression Regulation, Developmental/genetics/physiology
MH  - Gene Expression Regulation, Plant/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Meristem/genetics/metabolism
MH  - Plant Shoots/genetics/metabolism
MH  - Plants, Genetically Modified
MH  - RNA, Nuclear/genetics/*metabolism
PMC - PMC6945838
EDAT- 2019/11/15 06:00
MHDA- 2020/09/09 06:00
PMCR- 2019/11/13
CRDT- 2019/11/15 06:00
PHST- 2019/08/08 00:00 [received]
PHST- 2019/11/05 00:00 [accepted]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
PHST- 2019/11/15 06:00 [entrez]
PHST- 2019/11/13 00:00 [pmc-release]
AID - pp.19.00980 [pii]
AID - 201900980R1 [pii]
AID - 10.1104/pp.19.00980 [doi]
PST - ppublish
SO  - Plant Physiol. 2020 Jan;182(1):147-158. doi: 10.1104/pp.19.00980. Epub 2019 Nov 
      13.