PMID- 31723591
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220411
IS  - 2373-8731 (Print)
IS  - 2373-8731 (Electronic)
IS  - 2373-8731 (Linking)
VI  - 5
IP  - 6
DP  - 2019 Jun
TI  - Cold Storage Increases Albumin and Advanced Glycation-End Product-Albumin Levels 
      in Kidney Transplants: A Possible Cause for Exacerbated Renal Damage.
PG  - e454
LID - 10.1097/TXD.0000000000000897 [doi]
LID - e454
AB  - Prolonged cold storage (CS) of kidneys is associated with poor renal outcome 
      after transplantation (Tx). We recently showed that in rats (Lewis), proteasome 
      and renal function were severely compromised in kidney transplants subjected to 
      CS (CS/Tx) as compared with those without CS exposure (autotransplanted [ATx]). 
      METHODS: Evaluation of whole-kidney extracts from our rat kidney transplant model 
      showed a subset of proteins induced after CS/Tx when compared with ATx or sham 
      groups; this study examined those proteins using mass spectrometry, western 
      blotting, immunoprecipitation, and immunohistochemistry. RESULTS: Mass 
      spectrometry identified basal albumin levels in sham kidney extracts; western 
      blots and immunohistochemistry confirmed this. Western blotting showed 
      exceptionally higher albumin levels in both soluble and insoluble fractions of 
      CS/Tx renal extracts when compared with ATx and sham groups. Surprisingly, levels 
      of advanced glycation-end products (AGE) were higher in CS/Tx renal extracts. 
      Furthermore, immunoprecipitation of albumin followed by western blotting for AGE 
      revealed AGE-albumin in all 3 extracts; its levels were highest in CS/Tx 
      extracts. Immunohistochemistry analysis of kidney sections revealed higher 
      albumin or AGE levels in the CS/Tx group, and the protein was detected all over 
      (within glomeruli, and intratubular and extratubular compartments) when compared 
      with ATx and sham groups, which show confinement of these proteins to the 
      extratubular compartment and within glomeruli. As expected, kidneys of the ATx 
      group showed evidence of more macrophages, which was exacerbated in the CS/Tx 
      group. CONCLUSIONS: These results suggested that CS/Tx increased AGE-albumin, 
      which was correlated with increased inflammation and renal damage.
CI  - Copyright (c) 2019 The Author(s). Transplantation Direct. Published by Wolters 
      Kluwer Health, Inc.
FAU - Lo, Sorena
AU  - Lo S
AD  - Department of Pharmacology and Toxicology, University of Arkansas for Medical 
      Sciences, Little Rock, AR.
FAU - Byrum, Stephanie D
AU  - Byrum SD
AD  - Department of Biochemistry and Molecular Biology, University of Arkansas for 
      Medical Sciences, Little Rock, AR.
AD  - Center for Translational Pediatric Research, Arkansas Children's Research 
      Institute, Little Rock, AR.
FAU - Tackett, Alan J
AU  - Tackett AJ
AD  - Department of Biochemistry and Molecular Biology, University of Arkansas for 
      Medical Sciences, Little Rock, AR.
AD  - Center for Translational Pediatric Research, Arkansas Children's Research 
      Institute, Little Rock, AR.
FAU - Parajuli, Nirmala
AU  - Parajuli N
AD  - Department of Pharmacology and Toxicology, University of Arkansas for Medical 
      Sciences, Little Rock, AR.
AD  - Center for Translational Pediatric Research, Arkansas Children's Research 
      Institute, Little Rock, AR.
LA  - eng
GR  - 16SDG27600026/AHA/American Heart Association-American Stroke Association/United 
      States
GR  - P20 GM121293/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20190522
PL  - United States
TA  - Transplant Direct
JT  - Transplantation direct
JID - 101651609
PMC - PMC6791592
COIS- The authors declare no conflicts of interest.
EDAT- 2019/11/15 06:00
MHDA- 2019/11/15 06:01
PMCR- 2019/05/22
CRDT- 2019/11/15 06:00
PHST- 2019/03/04 00:00 [received]
PHST- 2019/04/01 00:00 [revised]
PHST- 2019/04/03 00:00 [accepted]
PHST- 2019/11/15 06:00 [entrez]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2019/11/15 06:01 [medline]
PHST- 2019/05/22 00:00 [pmc-release]
AID - 10.1097/TXD.0000000000000897 [doi]
PST - epublish
SO  - Transplant Direct. 2019 May 22;5(6):e454. doi: 10.1097/TXD.0000000000000897. 
      eCollection 2019 Jun.