PMID- 31725165
OWN - NLM
STAT- MEDLINE
DCOM- 20200131
LR  - 20200131
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 60
IP  - 14
DP  - 2019 Nov 1
TI  - Lowered Decorin With Aberrant Extracellular Matrix Remodeling in Aqueous Humor 
      and Tenon's Tissue From Primary Glaucoma Patients.
PG  - 4661-4669
LID - 10.1167/iovs.19-27091 [doi]
AB  - PURPOSE: To evaluate the inflammatory cytokine, growth factors, extracellular 
      matrix (ECM) remodeling genes, profibrotic and antifibrotic molecules in patients 
      undergoing glaucoma filtration surgery (GFS). Additionally, the effect of 
      preoperative antiglaucoma medications (AGMs) and postoperative bleb status were 
      related to these parameters. METHODS: Tenon's tissue and aqueous humour (AH) were 
      collected from 207 patients undergoing GFS with primary open-angle glaucoma 
      (POAG) (n = 77), primary angle-closure glaucoma (PACG) (n = 62), and cataract 
      controls (n = 68). Monocyte chemoattractant protein-1 (MCP-1), connective tissue 
      growth factor (CTGF), transforming growth factor beta1/2 (TGF-beta1/2), lysyl oxidase 
      (LOX), lysyl oxidase L2 (LOXL2), elastin (ELN), collagen type 1 alpha 1 (COL1A1), 
      secreted protein acidic and rich in cysteine (SPARC), alpha-smooth muscle actin 
      (alpha-SMA), and decorin (DCN) were determined in tenon's tissue by real-time PCR and 
      in AH using ELISA. RESULTS: A significant increase was observed in the 
      transcripts of MCP-1, TGF-beta2, and SPARC in POAG and PACG (P < 0.05); CTGF, 
      TGF-beta1, LOX, LOXL2, ELN, COL1A1, and alpha-SMA in PACG (P < 0.05) compared with 
      control. DCN transcript was significantly decreased in POAG and PACG (P < 0.05) 
      compared with control. The protein levels of CTGF, TGF-beta1/beta2, ELN, SPARC, and 
      LOXL2 was significantly elevated in POAG and PACG (P < 0.05); DCN was decreased 
      (P < 0.05) compared with control. These parameters showed significant association 
      with duration of preoperative AGMs and postoperative bleb status. CONCLUSIONS: 
      This study demonstrates increased expression of growth factors and ECM molecules, 
      both at protein and transcript levels in GFS patients. A decreased DCN in AH 
      seems striking, and if restored might have a therapeutic role in minimizing 
      postoperative scarring to improve GFS outcome.
FAU - NikhalaShree, Sampath
AU  - NikhalaShree S
AD  - R.S. Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision 
      Research Foundation, Sankara Nethralaya, Chennai, India.
AD  - School of Chemical and Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 
      India.
FAU - Karthikkeyan, Gayathree
AU  - Karthikkeyan G
AD  - R.S. Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision 
      Research Foundation, Sankara Nethralaya, Chennai, India.
FAU - George, Ronnie
AU  - George R
AD  - Smt Jadhavbai Nathmal Singhvee Glaucoma Services, Medical Research Foundation, 
      Sankara Nethralaya, Chennai, India.
FAU - Shantha, Balekudaru
AU  - Shantha B
AD  - Smt Jadhavbai Nathmal Singhvee Glaucoma Services, Medical Research Foundation, 
      Sankara Nethralaya, Chennai, India.
FAU - Vijaya, Lingam
AU  - Vijaya L
AD  - Smt Jadhavbai Nathmal Singhvee Glaucoma Services, Medical Research Foundation, 
      Sankara Nethralaya, Chennai, India.
FAU - Ratra, Vineet
AU  - Ratra V
AD  - Smt Jadhavbai Nathmal Singhvee Glaucoma Services, Medical Research Foundation, 
      Sankara Nethralaya, Chennai, India.
FAU - Sulochana, Konerirajapuram Natarajan
AU  - Sulochana KN
AD  - R.S. Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision 
      Research Foundation, Sankara Nethralaya, Chennai, India.
FAU - Coral, Karunakaran
AU  - Coral K
AD  - R.S. Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision 
      Research Foundation, Sankara Nethralaya, Chennai, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (CCN2 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (DCN protein, human)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Aged
MH  - Aqueous Humor/*metabolism
MH  - Case-Control Studies
MH  - Connective Tissue Growth Factor/genetics/metabolism
MH  - Cytokines/genetics/metabolism
MH  - Decorin/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Matrix/*metabolism
MH  - Extracellular Matrix Proteins/genetics/metabolism
MH  - Female
MH  - Glaucoma, Angle-Closure/*metabolism/surgery
MH  - Glaucoma, Open-Angle/*metabolism/surgery
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Tenon Capsule/*metabolism
MH  - Trabeculectomy
MH  - Transforming Growth Factor beta/genetics/metabolism
EDAT- 2019/11/15 06:00
MHDA- 2020/02/01 06:00
CRDT- 2019/11/15 06:00
PHST- 2019/11/15 06:00 [entrez]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2020/02/01 06:00 [medline]
AID - 2755674 [pii]
AID - 10.1167/iovs.19-27091 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2019 Nov 1;60(14):4661-4669. doi: 
      10.1167/iovs.19-27091.