PMID- 31725974
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200108
IS  - 2379-8068 (Electronic)
IS  - 2379-8068 (Linking)
VI  - 2
IP  - 1
DP  - 2017 Mar
TI  - Fluorescence In Situ Hybridization for Glycine max Metaphase Chromosomes.
PG  - 89-107
LID - 10.1002/cppb.20045 [doi]
AB  - This article presents protocols for fluorescence in situ hybridization (FISH) in 
      the cultivated soybean, Glycine max. The protocols represent soybean-optimized 
      versions developed for maize. We describe the use of two different probes types: 
      genomic-repeat-based fluorescently-tagged oligonucleotides and bacterial 
      artificial chromosomes (BACs). The two probe types can be used either 
      individually or together, depending on the experimental questions. The article 
      also includes starting points for executing FISH in additional legume species. (c) 
      2017 by John Wiley & Sons, Inc.
CI  - Copyright (c) 2017 John Wiley & Sons, Inc.
FAU - Findley, Seth D
AU  - Findley SD
AD  - University of Missouri, Division of Plant Sciences, Columbia, Missouri.
FAU - Birchler, James A
AU  - Birchler JA
AD  - University of Missouri, Division of Biological Sciences, Columbia, Missouri.
FAU - Stacey, Gary
AU  - Stacey G
AD  - University of Missouri, Division of Plant Sciences, Columbia, Missouri.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Plant Biol
JT  - Current protocols in plant biology
JID - 101685882
SB  - IM
OTO - NOTNLM
OT  - Glycine max
OT  - bacterial artificial chromosomes (BACs)
OT  - chromosome structure
OT  - fluorescence in situ hybridization (FISH)
OT  - genomic in situ hybridization (GISH)
OT  - karyotype
OT  - metaphase chromosomes
OT  - oligonucleotide probe
OT  - polyploidy
OT  - rolling-circle amplification (RCA)
EDAT- 2017/03/01 00:00
MHDA- 2017/03/01 00:01
CRDT- 2019/11/15 06:00
PHST- 2019/11/15 06:00 [entrez]
PHST- 2017/03/01 00:00 [pubmed]
PHST- 2017/03/01 00:01 [medline]
AID - 10.1002/cppb.20045 [doi]
PST - ppublish
SO  - Curr Protoc Plant Biol. 2017 Mar;2(1):89-107. doi: 10.1002/cppb.20045.