PMID- 31726113
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240227
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 282
DP  - 2019 Nov 11
TI  - Simultaneous detection of classical PRRSV, highly pathogenic PRRSV and 
      NADC30-like PRRSV by TaqMan probe real-time PCR.
PG  - 113774
LID - S0166-0934(19)30179-X [pii]
LID - 10.1016/j.jviromet.2019.113774 [doi]
AB  - Porcine Reproductive and Respiratory Syndrome (PRRS), an acute infectious disease 
      caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is one 
      of the most devastating diseases affecting the global swine industry. In order to 
      establish a multiplex real-time PCR method for the simultaneous detection of the 
      classical PRRSV (C-PRRSV) strain, the highly pathogenic PRRSV (HP-PRRSV) strain 
      and NADC30-like PRRSV (NL-PRRSV) strain, we designed specific primers and TaqMan 
      fluorescent probes based on the Nsp2 target gene sequence of these three 
      different PRRSV strains, and designed American-type PRRSV (PRRSV-U) special 
      primers and probes based on the relatively conserved target gene sequence of 
      ORF7. The method established in this study can quickly and accurately detect and 
      differentiate three types of strains of clinical tissue samples, respectively. 
      This method plays a key role in the rapid diagnosis and determination of PRRSV.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Qiu, Wenbin
AU  - Qiu W
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China; School of Life Sciences, Shandong Normal 
      University, Jinan, Jinan, 250014, China.
FAU - Meng, Kai
AU  - Meng K
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China; Shandong Key Laboratory of Poultry Diseases 
      Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of 
      Shandong Province, Institute of Poultry Science, Shandong Academy of Agricultural 
      Sciences, Jinan, 250023, China.
FAU - Liu, Yanyan
AU  - Liu Y
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Zhang, Yuyu
AU  - Zhang Y
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Wang, Zhao
AU  - Wang Z
AD  - China Institute of Veterinary Drug Control, 8 Nandajie, Zhongguancun, Haidian, 
      Beijing, 100081, China.
FAU - Chen, Zhi
AU  - Chen Z
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Yang, Jie
AU  - Yang J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Sun, Wenbo
AU  - Sun W
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Guo, Lihui
AU  - Guo L
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Ren, Sufang
AU  - Ren S
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Chen, Lei
AU  - Chen L
AD  - School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China.
FAU - Yang, Guiwen
AU  - Yang G
AD  - School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China.
FAU - Shi, Jianli
AU  - Shi J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Li, Jun
AU  - Li J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Du, Yijun
AU  - Du Y
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China.
FAU - Yu, Jiang
AU  - Yu J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China. Electronic address: yujiang_2213@163.com.
FAU - Wu, Jiaqiang
AU  - Wu J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Jinan, 250100, China; School of Life Sciences, Shandong Normal 
      University, Jinan, Jinan, 250014, China; Shandong Key Laboratory of Poultry 
      Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center 
      of Shandong Province, Institute of Poultry Science, Shandong Academy of 
      Agricultural Sciences, Jinan, 250023, China. Electronic address: 
      wujiaqiang2000@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20191111
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
OTO - NOTNLM
OT  - Classical PRRSV
OT  - Highly pathogenic PRRSV
OT  - NADC30-like PRRSV
OT  - Probe real-time PCR
OT  - TaqMan
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2019/11/15 06:00
MHDA- 2019/11/15 06:01
CRDT- 2019/11/15 06:00
PHST- 2019/04/16 00:00 [received]
PHST- 2019/10/29 00:00 [revised]
PHST- 2019/11/10 00:00 [accepted]
PHST- 2019/11/15 06:01 [medline]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2019/11/15 06:00 [entrez]
AID - S0166-0934(19)30179-X [pii]
AID - 10.1016/j.jviromet.2019.113774 [doi]
PST - aheadofprint
SO  - J Virol Methods. 2019 Nov 11;282:113774. doi: 10.1016/j.jviromet.2019.113774.