PMID- 31726114
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 276
DP  - 2020 Feb
TI  - Development of a real-time loop-mediated isothermal amplification (LAMP) assay 
      and visual LAMP assay for detection of African swine fever virus (ASFV).
PG  - 113775
LID - S0166-0934(19)30266-6 [pii]
LID - 10.1016/j.jviromet.2019.113775 [doi]
AB  - African swine fever (ASF) is a fatal disease caused by a virus in domestic pigs. 
      In this study, a real-time loop-mediated isothermal amplification (LAMP) assay 
      and visual LAMP assay were developed for the detection of African swine fever 
      virus (ASFV). LAMP primers targeting the p10 gene of ASFV were designed, the LAMP 
      reaction system was optimized with plasmid pUC57 containing the p10 gene 
      sequence, and the specificities of the real-time LAMP and the visual assays were 
      tested with the DNA or cDNA of pseudorabies virus (PRV), porcine circovirus type 
      2 (PCV2), classical swine fever virus (CSFV), porcine reproductive and 
      respiratory syndrome virus (PRRSV), porcine parvovirus (PPV) and ASFV, as well as 
      the plasmid pUC57 containing the p10 gene sequence. The detection limits were 
      determined using a serial dilution of plasmid pUC57 containing the p10 gene 
      sequence. Our results showed that the LAMP assays could accurately and 
      specifically detect ASFV with a detection limit of 30 copies per mul(-1) of pUC57 
      containing p10 gene sequence. In addition, the LAMP assays were further evaluated 
      using various genotypes of ASFV strains. Furthermore, the LAMP assays are 
      comparable with the well-established real-time PCR assay. This study provides 
      promising solutions for facilitating preliminary and cost-effective surveillance 
      for prevention and control of ASFV.
CI  - Published by Elsevier B.V.
FAU - Wang, Deguo
AU  - Wang D
AD  - Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety of 
      Henan Province, Xuchang University, Xuchang, 461000, China.
FAU - Yu, Jianghan
AU  - Yu J
AD  - School of Food and Biological Engineering, Henan University of Science and 
      Technology, Luoyang, 471000, China.
FAU - Wang, Yongzhen
AU  - Wang Y
AD  - Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety of 
      Henan Province, Xuchang University, Xuchang, 461000, China.
FAU - Zhang, Meng
AU  - Zhang M
AD  - School of Food and Biological Engineering, Henan University of Science and 
      Technology, Luoyang, 471000, China.
FAU - Li, Peng
AU  - Li P
AD  - XinXiang University, Xinxiang, 453000, China.
FAU - Liu, Meng
AU  - Liu M
AD  - Zhumadian Veterinary Drug & Feed (Animal Products) Inspection and Testing Center, 
      Zhumadian, 463000, China.
FAU - Liu, Yanhong
AU  - Liu Y
AD  - Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional 
      Research Center, Agricultural Research Service, United States Department of 
      Agriculture, Wyndmoor, PA, 19038, USA. Electronic address: yanhong.liu@usda.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191111
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - LAMP assay
SB  - IM
MH  - African Swine Fever/*diagnosis/virology
MH  - African Swine Fever Virus/genetics/*isolation & purification
MH  - Animals
MH  - Animals, Domestic/virology
MH  - DNA, Viral/*genetics
MH  - Limit of Detection
MH  - Molecular Diagnostic Techniques/*methods
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine/virology
OTO - NOTNLM
OT  - African swine fever (ASF)
OT  - African swine fever virus (ASFV)
OT  - Loop-mediated isothermal amplification (LAMP)
EDAT- 2019/11/15 06:00
MHDA- 2021/03/16 06:00
CRDT- 2019/11/15 06:00
PHST- 2019/07/09 00:00 [received]
PHST- 2019/11/05 00:00 [revised]
PHST- 2019/11/10 00:00 [accepted]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
PHST- 2019/11/15 06:00 [entrez]
AID - S0166-0934(19)30266-6 [pii]
AID - 10.1016/j.jviromet.2019.113775 [doi]
PST - ppublish
SO  - J Virol Methods. 2020 Feb;276:113775. doi: 10.1016/j.jviromet.2019.113775. Epub 
      2019 Nov 11.