PMID- 31726503
OWN - NLM
STAT- MEDLINE
DCOM- 20191126
LR  - 20200108
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 37
IP  - 10
DP  - 2019 Oct 20
TI  - [Antioxidant mechanism of diallyl sulfide in inhibiting leucopenia in peripheral 
      blood induced by benzene in rats].
PG  - 737-745
LID - 10.3760/cma.j.issn.1001-9391.2019.10.005 [doi]
AB  - Objective: To investigate the antioxidant mechanism of diallyl sulfide (DAS) in 
      antagonizing the reduction in peripheral blood white blood cells (WBC) induced by 
      benzene in rats. Methods: A total of 60 specific pathogen-free adult male 
      Sprague-Dawley rats, with a body weight of 180-220 g, were selected, and after 5 
      days of adaptive feeding, they were randomly divided into blank control group, 
      DAS control group, benzene model group, benzene+low-dose DAS group, 
      benzene+middle-dose DAS group, and benzene+high-dose DAS group, with 10 rats in 
      each group. The rats in the benzene+low-dose DAS group, the benzene+middle-dose 
      DAS group, the benzene+high-dose DAS group, and the DAS control group were given 
      DAS by gavage at a dose of 40, 80, 160, and 160 mg/kg.bw, respectively, and those 
      in the blank control group and the benzene model group were given an equal volume 
      of corn oil; 2 hours later, the rats in the benzene model group, the 
      benzene+low-dose DAS group, the benzene+middle-dose DAS group, and the 
      benzene+high-dose DAS group were given a mixture of benzene (1.3 g/kg.bw) and 
      corn oil (with a volume fraction of 50%), and those in the blank control group 
      and the DAS control group were given an equal volume of corn oil. The above 
      treatment was given once a day for 4 consecutive weeks. At 1 day before 
      treatment, anticoagulated blood was collected from the jugular vein for 
      peripheral blood cell counting. After anesthesia with intraperitoneally injected 
      pentobarbital (50 mg/kg.bw), blood samples were collected from the abdominal 
      aorta, serum was isolated, and the thymus, the spleen, and the femur were freed 
      at a low temperature to measure oxidative and antioxidant indices. The femur at 
      one side was freed for WBC counting in bone marrow. Results: Compared with the 
      blank control group, the benzene model group had significant reductions in the 
      volume, weight, and organ coefficient of the spleen and the thymus (P<0.05) ; 
      compared with the benzene model group, the benzene+low-dose DAS group, the 
      benzene+middle-dose DAS group, and the benzene+high-dose DAS group had 
      significant increases in the volume of the spleen and the thymus and the weight 
      and organ coefficient of the spleen (P<0.05), and the benzene+middle-dose DAS 
      group and the benzene+high-dose DAS group had significant increases in the weight 
      and organ coefficient of the thymus (P<0.05). Compared with the blank control 
      group, the benzene model group had a significant reduction in WBC count in 
      peripheral blood and bone marrow (P<0.05), and compared with the benzene model 
      group, the benzene+middle-dose DAS group and the benzene+high-dose DAS group had 
      a significant increase in WBC count in peripheral blood and bone marrow (P<0.05). 
      Compared with the blank control group, the benzene model group had a significant 
      increase in the serum level of malondialdehyde (MDA) (P<0.05) and significant 
      reductions in total superoxide dismutase (T-SOD) activity, reduced glutathione 
      (GSH) level, GSH/oxidized glutathione (GSSG) ratio, total antioxidant capacity 
      (T-AOC) (P<0.05) ; compared with the benzene model group, the benzene+high-dose 
      DAS group had a significant reduction in the serum level of MDA and significant 
      increases in T-SOD activity, GSH level, GSH/GSSG ratio, and T-AOC (P<0.05). 
      Compared with the blank control group, the benzene model group had a significant 
      increase in the level of MDA (P<0.05) and significant reductions in GSH level, 
      GSH/GSSG ratio, and T-AOC (P<0.05) in the spleen; compared with the benzene model 
      group, the benzene+low-dose DAS group, the benzene+middle-dose DAS group, and the 
      benzene+high-dose DAS group had a significant reduction in MDA level (P<0.05) and 
      significant increases in GSH level and T-AOC (P<0.05), and the benzene+high-dose 
      DAS group had significant increases in T-SOD activity and GSH/GSSG ratio 
      (P<0.05). Compared with the blank control group, the benzene model group had a 
      significant increase in the level of MDA in bone marrow cells (BMCs) and 
      peripheral blood mononucleated cells (PBMCs) (P<0.05) and a significant reduction 
      in T-AOC in PBMCs (P<0.05) ; compared with the benzene model group, the 
      benzene+low-dose DAS group, the benzene+middle-dose DAS group, and the 
      benzene+high-dose DAS group had a significant reduction in the level of MDA in 
      BMCs and PBMCs (P<0.05), and the benzene+high-dose DAS group had significant 
      increases in GSH level and GSH/GSSG ratio (P<0.05) . Conclusion: DAS can 
      antagonize the benzene-induced reduction in peripheral blood WBC, possibly by 
      exerting an anti-oxidative stress effect.
FAU - Yu, T
AU  - Yu T
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan 
      250012, China.
FAU - Wang, Q
AU  - Wang Q
FAU - Li, X J
AU  - Li XJ
FAU - Li, M
AU  - Li M
FAU - Liu, Z D
AU  - Liu ZD
FAU - Xie, K Q
AU  - Xie KQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (Allyl Compounds)
RN  - 0 (Antioxidants)
RN  - 0 (Sulfides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 60G7CF7CWZ (allyl sulfide)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
RN  - J64922108F (Benzene)
SB  - IM
MH  - Allyl Compounds/*pharmacology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Benzene/adverse effects
MH  - Glutathione/analysis
MH  - Leukopenia/chemically induced/*drug therapy
MH  - Male
MH  - Malondialdehyde/analysis
MH  - Oxidative Stress
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sulfides/*pharmacology
MH  - Superoxide Dismutase/analysis
OTO - NOTNLM
OT  - Benzene
OT  - Bone marrow cells
OT  - Diallyl sulfide
OT  - Leukopenia
OT  - Oxidative stress
OT  - Peripheral blood mononuclear cells
OT  - Rats
EDAT- 2019/11/15 06:00
MHDA- 2019/11/27 06:00
CRDT- 2019/11/15 06:00
PHST- 2019/11/15 06:00 [entrez]
PHST- 2019/11/15 06:00 [pubmed]
PHST- 2019/11/27 06:00 [medline]
AID - 10.3760/cma.j.issn.1001-9391.2019.10.005 [doi]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2019 Oct 20;37(10):737-745. doi: 
      10.3760/cma.j.issn.1001-9391.2019.10.005.