PMID- 31726576
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20191217
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 688
DP  - 2019 Oct 20
TI  - Impact of biochar amendment on the abundance and structure of diazotrophic 
      community in an alkaline soil.
PG  - 944-951
LID - S0048-9697(19)32866-9 [pii]
LID - 10.1016/j.scitotenv.2019.06.293 [doi]
AB  - Biological nitrogen (N) fixation contributes to the pool of plant-available N in 
      soil and helps to minimize the use of inorganic N fertilizer in agricultural 
      ecosystems. Although diazotrophs play an important role in the biological 
      fixation of atmospheric N(2) in a range of soil types, the knowledge of their 
      response to biochar amendment is still limited. Here, using the nifH gene as a 
      molecular marker, we investigated the short-term effect of biochar application on 
      the abundance, community composition and activity of diazotroph in an alkaline 
      soil. A field trial was established before soybean sowing in 2017 and five 
      treatments were included: inorganic NPK fertilizer (CK); inorganic NPK fertilizer 
      + wheat straw (CS); inorganic NPK fertilizer + low rate of biochar (B4); 
      inorganic NPK fertilizer + high rate of biochar (B20); biochar compound 
      fertilizer (BCF). The field trial was lasted for one crop season and samples were 
      collected by soybean harvest. The results showed that biochar addition generally 
      increased the concentration of soil organic carbon (SOC) and available 
      phosphorous (AP), while B20 treatment significantly increased the total nitrogen 
      (TN) and available potassium (AK). Biochar addition treatments increased the nifH 
      gene abundance and altered the community structure of soil diazotrophs. The 
      abundance of nifH gene was positively correlated with SOC, indicating that 
      increasing SOC potentially affected diazotrophic population in the alkaline soil. 
      Community structure of diazotrophs in the CS treatment was similar with the CK 
      treatment; thus, there was no effect of crop straw on diazotroph community 
      structure. In contrast, the application of biochar and biochar compound 
      fertilizer altered the diazotroph community structure with shifts in the dominant 
      genus, with higher Sinorhizobium in the biochar-amended treatments. SOC, C/N and 
      AP were the key factors correlated with change in diazotroph community structure. 
      Overall, our results suggest that the addition of biochar or biochar compound 
      fertilizer could increase the abundance and alter the community structure of 
      diazotrophs, which may benefit N fixation in alkaline agricultural soil. 
      Conversely, the direct straw return had no effect on the abundance and community 
      structure of diazotrophs.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Liu, Xiaoyu
AU  - Liu X
AD  - Institute of Resources, Ecosystem and Environment of Agriculture, Nanjing 
      Agricultural University, 1 Weigang, Nanjing 210095, China.
FAU - Liu, Cheng
AU  - Liu C
AD  - Institute of Resources, Ecosystem and Environment of Agriculture, Nanjing 
      Agricultural University, 1 Weigang, Nanjing 210095, China.
FAU - Gao, Wenhui
AU  - Gao W
AD  - Department of Bioengineering, College of Life Science, Huaibei Normal University, 
      Huaibei 235000, Anhui, China.
FAU - Xue, Chen
AU  - Xue C
AD  - Department of Bioengineering, College of Life Science, Huaibei Normal University, 
      Huaibei 235000, Anhui, China.
FAU - Guo, Zonghao
AU  - Guo Z
AD  - Department of Bioengineering, College of Life Science, Huaibei Normal University, 
      Huaibei 235000, Anhui, China.
FAU - Jiang, Li
AU  - Jiang L
AD  - State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology 
      and Geography, Chinese Academy of Science, Urumqi 830011, China.
FAU - Li, Feng
AU  - Li F
AD  - Department of Bioengineering, College of Life Science, Huaibei Normal University, 
      Huaibei 235000, Anhui, China. Electronic address: rx2500@163.com.
FAU - Liu, Yuan
AU  - Liu Y
AD  - Department of Bioengineering, College of Life Science, Huaibei Normal University, 
      Huaibei 235000, Anhui, China. Electronic address: 13856180050@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190621
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Fertilizers)
RN  - 0 (Soil)
RN  - 0 (biochar)
RN  - 16291-96-6 (Charcoal)
RN  - 27YLU75U4W (Phosphorus)
RN  - 7440-44-0 (Carbon)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Agriculture
MH  - Carbon
MH  - *Charcoal
MH  - *Ecosystem
MH  - *Fertilizers
MH  - Nitrogen/analysis
MH  - Phosphorus
MH  - Soil/chemistry
MH  - *Soil Microbiology
MH  - Triticum
OTO - NOTNLM
OT  - Biochar
OT  - Biological N fixation
OT  - Diazotroph
OT  - Soybean
OT  - nifH gene
EDAT- 2019/11/16 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/11/16 06:00
PHST- 2019/02/26 00:00 [received]
PHST- 2019/06/11 00:00 [revised]
PHST- 2019/06/19 00:00 [accepted]
PHST- 2019/11/16 06:00 [entrez]
PHST- 2019/11/16 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
AID - S0048-9697(19)32866-9 [pii]
AID - 10.1016/j.scitotenv.2019.06.293 [doi]
PST - ppublish
SO  - Sci Total Environ. 2019 Oct 20;688:944-951. doi: 10.1016/j.scitotenv.2019.06.293. 
      Epub 2019 Jun 21.