PMID- 31731179
OWN - NLM
STAT- MEDLINE
DCOM- 20200602
LR  - 20200602
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 41
DP  - 2019 Dec
TI  - Simulated microgravity-cultured mesenchymal stem cells improve recovery following 
      spinal cord ischemia in rats.
PG  - 101601
LID - S1873-5061(19)30231-4 [pii]
LID - 10.1016/j.scr.2019.101601 [doi]
AB  - Spinal cord ischemia is a potential complication of thoracoabdominal aortic 
      surgery that may induce irreversible motor disability. We investigated the 
      therapeutic efficacy of simulated microgravity-cultured mesenchymal stem cell 
      (MSC) injection following spinal cord ischemia-reperfusion injury. Sprague-Dawley 
      rats were divided into sham, phosphate-buffered saline (PBS), normal 
      gravity-cultured MSC (MSC-1 G), and simulated microgravity-cultured MSC (MSC-MG) 
      groups. Spinal cord ischemia was induced by transient balloon occlusion of the 
      thoracic aorta, which was followed immediately by PBS or MSC injection into the 
      left carotid artery. Hindlimb motor function was evaluated by the 
      Basso-Beattie-Bresnahan (BBB) scale. Spinal cords were removed 1, 3, or 7 days 
      post-injury for immunohistochemical staining and Western blot analysis. One day 
      post-injury, a few infiltrating inflammatory cells and small vacuoles were 
      observed without significant group differences, followed over several days by 
      progressive spinal cord degeneration. Glial fibrillary acidic protein 
      (GFAP)-positive (reactive) astrocyte numbers were increased in all three groups, 
      and brain-derived neurotrophic factor (BDNF) was colocalized with GFAP-positive 
      cells in spinal ventral horn. Animals in the MSC-MG group demonstrated greater 
      BDNF-positive astrocyte numbers, reduced caspase-3-positive cell numbers, and 
      superior motor recovery. Microgravity-cultured MSC-based therapy may improve 
      functional recovery following spinal ischemia-reperfusion injury by promoting 
      astrocytic BDNF release, thereby preventing apoptosis.
CI  - Copyright (c) 2019 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Kurose, Tomoyuki
AU  - Kurose T
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Takahashi, Shinya
AU  - Takahashi S
AD  - Department of Cardiovascular Surgery, Hiroshima University Hospital, Hiroshima, 
      Japan.
FAU - Otsuka, Takashi
AU  - Otsuka T
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Nakagawa, Kei
AU  - Nakagawa K
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Imura, Takeshi
AU  - Imura T
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Sueda, Taijiro
AU  - Sueda T
AD  - Hiroshima City Medical Association-administered Hiroshima City Aki Hospital, 
      Hiroshima, Japan.
FAU - Yuge, Louis
AU  - Yuge L
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan. Electronic address: 
      ryuge@hiroshima-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20191015
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
SB  - IM
MH  - Allografts
MH  - Animals
MH  - Ischemia/metabolism/pathology/*therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Recovery of Function
MH  - Spinal Cord/blood supply/*metabolism/pathology
MH  - Spinal Cord Diseases/metabolism/pathology/*therapy
MH  - *Weightlessness
OTO - NOTNLM
OT  - Apoptosis
OT  - Astrocyte
OT  - Brain-derived neurotrophic factor
OT  - Ischemia-reperfusion injury
OT  - Microgravity
OT  - Motor function
OT  - Spinal cord
EDAT- 2019/11/16 06:00
MHDA- 2020/06/03 06:00
CRDT- 2019/11/16 06:00
PHST- 2019/04/03 00:00 [received]
PHST- 2019/09/14 00:00 [revised]
PHST- 2019/09/23 00:00 [accepted]
PHST- 2019/11/16 06:00 [pubmed]
PHST- 2020/06/03 06:00 [medline]
PHST- 2019/11/16 06:00 [entrez]
AID - S1873-5061(19)30231-4 [pii]
AID - 10.1016/j.scr.2019.101601 [doi]
PST - ppublish
SO  - Stem Cell Res. 2019 Dec;41:101601. doi: 10.1016/j.scr.2019.101601. Epub 2019 Oct 
      15.