PMID- 3173402
OWN - NLM
STAT- MEDLINE
DCOM- 19881117
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 209
IP  - 1-2
DP  - 1988 Sep-Oct
TI  - Differential effects induced by N-hydroxy-2-acetylaminofluorene and 
      N-hydroxy-2-aminofluorene in DNA excision-repair-deficient Chinese hamster cells.
PG  - 45-50
AB  - The direct-acting cytotoxic properties of N-hydroxy-2-acetylaminofluorene 
      (N-OH-AAF) and N-hydroxy-2-aminofluorene (N-OH-AF) have been determined in 
      repair-proficient (AA8-4) and repair-deficient (UV-5) Chinese hamster ovary 
      cells. Cytotoxicity comparisons indicate that UV-5 cells are considerably more 
      sensitive to exposure to N-OH-AAF than is the parental AA8-4 cell line, i.e., 
      concentrations needed to obtain a D37 for survival of AA8-4 is greater than 
      5-fold higher than for UV-5. Mutation analysis at the HGPRT locus also indicates 
      the increased sensitivity of UV-5 cells to N-OH-AAF as witnessed by an enhanced 
      induction of 6-thioguanine-resistant colonies at equitoxic doses. Conversely, 
      N-OH-AAF, did not induce a 'UV-mimetic' response when comparing genotoxicity 
      between these two cell lines. Our data coupled with previously published 
      model-building and adduct removal studies (Broyde and Hingerty, 1983; Fuchs and 
      Daune, 1974; Grunberger and Weinstein, 1976; Yamasaki et al., 1977) suggest that 
      the minor DNA adduct species, N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene, 
      may be responsible for the hypermutagenicity witnessed in DNA 
      excision-repair-deficient cells treated with N-OH-AAF.
FAU - Okinaka, R T
AU  - Okinaka RT
AD  - Life Sciences Division, Los Alamos National Laboratory, NM 87545.
FAU - Whaley, T W
AU  - Whaley TW
FAU - Strniste, G F
AU  - Strniste GF
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Fluorenes)
RN  - 53-95-2 (Hydroxyacetylaminofluorene)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
RN  - 9M98QLJ2DL (2-Acetylaminofluorene)
SB  - IM
MH  - 2-Acetylaminofluorene/*analogs & derivatives
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cricetinae
MH  - *DNA Repair
MH  - Fluorenes/*toxicity
MH  - Hydroxyacetylaminofluorene/*toxicity
MH  - Mutation/*drug effects
MH  - Ultraviolet Rays
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 0165-7992(88)90109-1 [pii]
AID - 10.1016/0165-7992(88)90109-1 [doi]
PST - ppublish
SO  - Mutat Res. 1988 Sep-Oct;209(1-2):45-50. doi: 10.1016/0165-7992(88)90109-1.