PMID- 31734285
OWN - NLM
STAT- MEDLINE
DCOM- 20201116
LR  - 20201116
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 98
DP  - 2020 Mar
TI  - Novel functions of grass carp three p40 isoforms as modulators of Th17 signature 
      cytokine expression in head kidney leukocytes.
PG  - 995-1000
LID - S1050-4648(19)31068-X [pii]
LID - 10.1016/j.fsi.2019.11.025 [doi]
AB  - Interleukin (IL)-12p40, a component of IL-12 and IL-23, can be secreted as 
      monomer and homodimer in mammals. Our previous study has proved the existence of 
      natural three p40 isoforms and their proinflammatory properties in grass carp. In 
      the present study, we unexpectedly found that recombinant grass carp p40a/b/c 
      (rgcp40a, rgcp40b and rgcp40c) were able to enhance the mRNA levels of grass carp 
      il-17a/f1 (gcil-17a/f1) in a dose- and time-dependent manner in head kidney 
      leukocytes (HKLs). In agreement with these findings, the enzyme-linked 
      immunosorbent assay (ELISA) showed that rgcp40a, rgcp40b and rgcp40c markedly 
      stimulated gcIl-17a/f1 secretion from the HKLs. Together with their stimulatory 
      effects on grass carp gcil-22 and gcil-26 expression, our data suggested their 
      potential to mediate Th17-like response in grass carp. To support this notion, we 
      investigated the underlying mechanisms for the regulation of rgcp40 isoforms on 
      gcil-17a/f1 expression, and found that three rgcp40 isoforms significantly 
      induced the activation of Erk, Jnk and Stat3 pathways in a time-dependent 
      oscillation in the same cell model. Moreover, three rgcp40 isoforms-induced 
      gcil-17a/f1 mRNA expression was suppressed by the inhibition on Erk, Jnk and 
      Stat3 pathways, suggesting the signaling pathways in the p40 isoforms-mediating 
      il-17a/f1 transcription. These studies for the first time proved the involvement 
      of three gcp40 isoforms in mediating Th17 signature cytokine expression in fish 
      immune cells, therefore providing new insights into the roles of p40 in teleost 
      immunity.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Feng, Shiyu
AU  - Feng S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China. Electronic address: 
      zhouhongzh@uestc.edu.cn.
FAU - Wang, Yanyan
AU  - Wang Y
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Qiu, Xingyang
AU  - Qiu X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhang, Anying
AU  - Zhang A
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20191114
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - Carps/*genetics/immunology
MH  - Cytokines/*genetics/immunology
MH  - Fish Proteins/*genetics/immunology
MH  - Gene Expression/*immunology
MH  - Head Kidney/*immunology
MH  - Leukocytes/*immunology
MH  - Th17 Cells/immunology
OTO - NOTNLM
OT  - Grass carp
OT  - IL12/IL23p40 subunit
OT  - Il-17a/f1
OT  - Isoforms
OT  - Signaling pathway
OT  - Th17-like response
EDAT- 2019/11/18 06:00
MHDA- 2020/11/18 06:00
CRDT- 2019/11/18 06:00
PHST- 2019/06/24 00:00 [received]
PHST- 2019/11/07 00:00 [revised]
PHST- 2019/11/13 00:00 [accepted]
PHST- 2019/11/18 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2019/11/18 06:00 [entrez]
AID - S1050-4648(19)31068-X [pii]
AID - 10.1016/j.fsi.2019.11.025 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 Mar;98:995-1000. doi: 10.1016/j.fsi.2019.11.025. 
      Epub 2019 Nov 14.