PMID- 31734861
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20231113
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Print)
IS  - 1234-1983 (Linking)
VI  - 61
IP  - 1
DP  - 2020 Feb
TI  - Molecular and cytogenetic description of somatic hybrids between Gentiana 
      cruciata L. and G. tibetica King.
PG  - 13-24
LID - 10.1007/s13353-019-00530-x [doi]
AB  - Somatic hybridization provides an opportunity to create cells with new genetic 
      constitution. Here, the interspecific somatic hybrid plants regenerated in vitro 
      following fusion of cell suspension-derived protoplasts of tetraploid Cross 
      Gentian (Gentiana cruciata L., 2n = 52) with protoplasts released from mesophyll 
      tissue of another tetraploid species, Tibetan Gentian (G. tibetica King, 2n = 
      52), were studied. According to the results of genome analyses with AFLP, ISSR, 
      and CAPS markers, all somatic hybrids were genetically closer to "suspension" 
      fusion partner G. cruciata than to "mesophyll" partner G. tibetica, but they got 
      G. tibetica chloroplasts. Chromosome counting revealed little variation in the 
      number of chromosomes in hybrid's cells (2n = 88 or 2n = 90), although all plants 
      possessed similar nuclear DNA content which remained stable even after 2 years of 
      in vitro culture. Fluorescence in situ hybridization (FISH) showed that hybrids 
      possessed 4 to 7 chromosomes bearing 5S rDNA sites and 6 or 7 chromosomes with 
      35S rDNA sites. A part of FISH signals was smaller than those observed in the 
      parental species, which could indicate the loss of rDNA sequences. Genomic in 
      situ hybridization (GISH) showed the predominance of the number of G. cruciata 
      chromosomes over chromosomes of G. tibetica. However, a significant level of 
      cross-hybridization was observed for about one-third of hybrid chromosomes, 
      indicating a high degree of homeology between the genomes of G. cruciata and G. 
      tibetica.
FAU - Tomiczak, Karolina
AU  - Tomiczak K
AUID- ORCID: 0000-0002-4529-5581
AD  - Department of Conservation Biology of Plants, Polish Academy of Sciences 
      Botanical Garden - Center for Biological Diversity Conservation in Powsin, 
      Prawdziwka 2, 02-973, Warsaw, Poland. k.tomiczak@obpan.pl.
LA  - eng
GR  - Grant No. 3P04C 037 23/Polish Ministry of Science and Higher Education/
PT  - Journal Article
DEP - 20191116
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Amplified Fragment Length Polymorphism Analysis
MH  - *Chromosomes, Plant
MH  - *Cytogenetic Analysis
MH  - DNA, Ribosomal/genetics
MH  - Genetic Markers
MH  - *Genome, Plant
MH  - *Genomics/methods
MH  - Gentiana/classification/*genetics
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Microsatellite Repeats
PMC - PMC6968988
OTO - NOTNLM
OT  - AFLP
OT  - CAPS
OT  - Electrofusion
OT  - Gentian
OT  - ISSR
OT  - In situ hybridization
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/11/18 06:00
MHDA- 2020/06/09 06:00
PMCR- 2019/11/16
CRDT- 2019/11/18 06:00
PHST- 2019/05/29 00:00 [received]
PHST- 2019/10/10 00:00 [accepted]
PHST- 2019/09/30 00:00 [revised]
PHST- 2019/11/18 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/11/18 06:00 [entrez]
PHST- 2019/11/16 00:00 [pmc-release]
AID - 10.1007/s13353-019-00530-x [pii]
AID - 530 [pii]
AID - 10.1007/s13353-019-00530-x [doi]
PST - ppublish
SO  - J Appl Genet. 2020 Feb;61(1):13-24. doi: 10.1007/s13353-019-00530-x. Epub 2019 
      Nov 16.