PMID- 31736005
OWN - NLM
STAT- MEDLINE
DCOM- 20200603
LR  - 20201209
IS  - 2092-9293 (Electronic)
IS  - 1976-9571 (Linking)
VI  - 42
IP  - 1
DP  - 2020 Jan
TI  - Oral administration of Ulmus davidiana extract suppresses interleukin-1beta 
      expression in LPS-induced immune responses and lung injury.
PG  - 87-95
LID - 10.1007/s13258-019-00883-x [doi]
AB  - BACKGROUND: Ulmus davidiana (UD) is a traditional Korean herb medicine that is 
      used to treat inflammatory disorders. UD has been shown to modulate a number of 
      inflammatory processes in vitro or in vivo studies. However, the molecular 
      mechanisms of UD on lipopolysaccharide (LPS)-induced acute lung injury remain to 
      be understood. OBJECTIVE: The primary objective of this study is to determine the 
      effect of UD bark water extract on LPS-induced immune responses and lung injury 
      using both in vitro and in vivo models. METHODS: RAW 264.7 cells and a rat model 
      of acute lung injury (ALI) were used to study the effects of UD on several 
      parameters. Nitrite level, lactate dehydrogenase (LDH) level, and superoxide 
      dismutase (SOD) activities were measured. Tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-1beta (IL-1beta), and plasma transaminase activities in blood were also 
      determined. Pathological investigations were also performed. RESULTS: LPS 
      infusion resulted in elevated IL-1beta mRNA expression, nitrite levels, TNF-alpha 
      expression, and IL-1beta expression in RAW 264.7 cells. LPS infusion also increased 
      levels of nitrite/nitrate, total protein, LDH, and TNF-alpha in bronchoalveolar 
      lavage fluid, but reduced SOD levels in ex vivo and in vivo models. UD 
      administration ameliorated all these inflammatory markers. In particular, 
      treatment with UD reduced LPS-induced nitrite production in RAW 264.7 cells in a 
      dose-dependent manner. UD treatment also counteracted the LPS-induced increase in 
      alanine aminotransferase (ALT) and aspartate transaminase (AST) activity in rat 
      plasma, leading to a significant reduction in ALT and AST activity. CONCLUSIONS: 
      The results revealed that UD treatment reduces LPS-induced nitrite production, 
      IL-1beta mRNA expression, and TNF-alpha expression. In addition, LPS-induced decrease in 
      SOD level is significantly elevated by UD administration. These results indicate 
      that UD extract merits consideration as a potential drug for treating and/or 
      preventing ALI.
FAU - Park, Kwang-Hyun
AU  - Park KH
AUID- ORCID: 0000-0001-8816-2976
AD  - Department of Oriental Pharmaceutical Development, Nambu University, Gwangju, 
      62271, Republic of Korea.
AD  - Department of Emergency Medical Rescue, Nambu University, Gwangju, 62271, 
      Republic of Korea.
FAU - Chung, Eun-Yong
AU  - Chung EY
AD  - Department of Anesthesiology and Pain Medicine, Bucheon St. Mary's Hospital, 
      Catholic University of Korea, Bucheon, 14647, Republic of Korea.
FAU - Choi, Yu-Na
AU  - Choi YN
AD  - Department of Anesthesiology and Pain Medicine, Bucheon St. Mary's Hospital, 
      Catholic University of Korea, Bucheon, 14647, Republic of Korea.
FAU - Jang, Hye-Yeon
AU  - Jang HY
AD  - Department of Biochemistry, Chonbuk National University Medical School, Jeonju, 
      54907, Republic of Korea.
FAU - Kim, Jong-Suk
AU  - Kim JS
AD  - Department of Biochemistry, Chonbuk National University Medical School, Jeonju, 
      54907, Republic of Korea. jsukim@chonbuk.ac.kr.
FAU - Kim, Gi-Beum
AU  - Kim GB
AD  - Eouidang Agricultural Company, Wanju, 55360, Republic of Korea. 
      biomedico@naver.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191117
PL  - Korea (South)
TA  - Genes Genomics
JT  - Genes & genomics
JID - 101481027
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Acute Lung Injury/chemically induced/immunology/metabolism/*prevention & control
MH  - Administration, Oral
MH  - Animals
MH  - Gene Expression Regulation/*drug effects
MH  - Interleukin-1beta/genetics/*metabolism
MH  - Lipopolysaccharides/*toxicity
MH  - Male
MH  - Mice
MH  - Plant Extracts/*administration & dosage/pharmacology
MH  - RAW 264.7 Cells
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Respiratory Distress Syndrome/chemically 
      induced/immunology/metabolism/*prevention & control
MH  - Ulmus/*chemistry
OTO - NOTNLM
OT  - Acute lung injury
OT  - Animal model
OT  - Lipopolysaccharide
OT  - Ulmus davidiana
EDAT- 2019/11/19 06:00
MHDA- 2020/06/04 06:00
CRDT- 2019/11/19 06:00
PHST- 2019/07/19 00:00 [received]
PHST- 2019/10/28 00:00 [accepted]
PHST- 2019/11/19 06:00 [pubmed]
PHST- 2020/06/04 06:00 [medline]
PHST- 2019/11/19 06:00 [entrez]
AID - 10.1007/s13258-019-00883-x [pii]
AID - 10.1007/s13258-019-00883-x [doi]
PST - ppublish
SO  - Genes Genomics. 2020 Jan;42(1):87-95. doi: 10.1007/s13258-019-00883-x. Epub 2019 
      Nov 17.