PMID- 31736789
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 10
DP  - 2019
TI  - Evaluation of the Expression and Function of the TRE2-like and TRE2 Genes in 
      Ecdysis of Harmonia axyridis.
PG  - 1371
LID - 10.3389/fphys.2019.01371 [doi]
LID - 1371
AB  - Harmonia axyridis is an important predatory insect and widely used in biological 
      control of agricultural and forestry pests. Trehalose is directly involved in the 
      energy storage of the H. axyridis and in the oxidative function of various 
      physiological activities thereby providing an energy source for its growth and 
      development. The aim of this study was to explore the potential function of 
      membrane-bound-like trehalase (TRE2-like) and membrane-bound trehalase (TRE2) 
      genes in H. axyridis by RNAi. In addition, the activity of soluble and 
      membrane-bound trehalase and the expression of genes related to trehalose and 
      glycogen metabolism were determined in the larvae injected with dsTRE2-like or 
      dsTRE2. The results showed that wing abnormality and mortality appeared in 
      adults, as well as the activity of soluble trehalase and glycogen contents 
      increased when interfering with TRE2-like gene. However, the activity of 
      membrane-bound trehalase, trehalose and glucose contents in the larvae decreased. 
      The expression of glycogen synthase (GS) and glycogen phosphorylase (GP) genes 
      were decreased after RNAi in the ecdysis stage. The expression of chitin synthase 
      gene A (CHSA), chitin synthase gene B (CHSB), and trehalose-6-phosphate synthase 
      genes (TPS) were decreased significantly after RNAi, especially in the ecdysis 
      stage. These results indicated that RNA interference is capable of knocking down 
      gene expression of TRE2-like and TRE2, thereby disrupting trehalose metabolism 
      which affects the chitin synthesis pathway in turn and also leads to 
      developmental defects, such as wing deformities. This study could provide some 
      theoretical guidance for the function of TRE2 gene in other insects.
CI  - Copyright (c) 2019 Li, Chen, Wang, Pan, Wang, Wang and Tang.
FAU - Li, Yan
AU  - Li Y
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      China.
FAU - Chen, Xu
AU  - Chen X
AD  - Institute of Plant and Environment Protection, Beijing Academy of Agriculture and 
      Forestry Sciences, Beijing, China.
FAU - Wang, Sha-Sha
AU  - Wang SS
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      China.
FAU - Pan, Bi-Ying
AU  - Pan BY
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      China.
FAU - Wang, Shi-Gui
AU  - Wang SG
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      China.
FAU - Wang, Su
AU  - Wang S
AD  - Institute of Plant and Environment Protection, Beijing Academy of Agriculture and 
      Forestry Sciences, Beijing, China.
FAU - Tang, Bin
AU  - Tang B
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20191101
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC6839538
OTO - NOTNLM
OT  - Harmonia axyridis
OT  - RNA interference
OT  - TRE2
OT  - TRE2-like
OT  - physiological activities
EDAT- 2019/11/19 06:00
MHDA- 2019/11/19 06:01
PMCR- 2019/11/01
CRDT- 2019/11/19 06:00
PHST- 2019/09/06 00:00 [received]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/11/19 06:00 [entrez]
PHST- 2019/11/19 06:00 [pubmed]
PHST- 2019/11/19 06:01 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - 10.3389/fphys.2019.01371 [doi]
PST - epublish
SO  - Front Physiol. 2019 Nov 1;10:1371. doi: 10.3389/fphys.2019.01371. eCollection 
      2019.