PMID- 31738890
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200413
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 679
DP  - 2020 Jan 15
TI  - Micro RNA 146a gene variant / TNF-alpha / IL-6 / IL-1 beta; A cross-link axis inbetween 
      oxidative stress, endothelial dysfunction and neuro-inflammation in acute 
      ischemic stroke and chronic schizophrenic patients.
PG  - 108193
LID - S0003-9861(19)30761-1 [pii]
LID - 10.1016/j.abb.2019.108193 [doi]
AB  - This work was purposed to speculate the possible association of 
      rs2910164hsa-miR-146a C>G gene single nucleotide polymorphism in the pathogenesis 
      of schizophrenia and subsequently their relevance to neuro-inflammatory, vascular 
      and oxidative stress pathways as acute ischemic stroke (AIS) risk factors in 
      chronic schizophrenic patients. 450 subjects, 150 healthy controls (group I), 150 
      chronic schizophrenic patients without any evidences of stroke (group II) and 150 
      chronic schizophrenic patients with AIS (group III) were included. Genotypes (CC, 
      CG&GG) for hsa-mir-146a gene polymorphism were identified using polymerase chain 
      reaction-restriction fragment length polymorphism PCR-RFLP technique. Tumor 
      necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), interleukin 1beta (IL-1 beta), 
      plasminogen activator-inhibitor 1 (PAI-1), thrombomodulin (TM) and 
      8-Hydroxy-2-deoxyguanosine (8-OHdG) serum levels were immunoassayed. Complete 
      lipid profile was estimated. The CG and GG hsa-miR-146a genotypes were associated 
      with increased risk of both schizophrenia and AIS in schizophrenic patients with 
      thrombomodulin levels decrement in group II& III. On the other side, the risk 
      genotypes were associated significantly with positive and negative syndrome scale 
      PANSS scores, TNF-alpha, IL-6, IL-1 beta, PAI-1, and 8-OHdG increment levels in both 
      groups II & III. By contrast, the CG and GG hsa-miR-146a genotypes did not affect 
      the neuro-inflammatory and oxidative stress markers in healthy controls. These 
      findings illustrate a new mechanism strengthening the occurrence of oxidative 
      stress and DNA damage as a result of the neuro-inflammatory and endothelial 
      dysfunction status originated from the hsa-miR-146a C>G gene single nucleotide 
      polymorphism, thus, confirming their role in the pathogenesis of schizophrenia 
      and its AIS risk.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Ibrahim, Rowida Raafat
AU  - Ibrahim RR
AD  - Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Tanta 
      University, Egypt. Electronic address: rowaida.yousef@med.tanta.edu.eg.
FAU - Amer, Reham A
AU  - Amer RA
AD  - Neuro-psychiatry Department, Faculty of Medicine, Tanta University, Egypt.
FAU - Abozeid, Abeer A
AU  - Abozeid AA
AD  - Physiology Department, Faculty of Medicine, Tanta University, Egypt.
FAU - Elsharaby, Radwa Mahmoud
AU  - Elsharaby RM
AD  - Clinical Pathology Department, Faculty of Medicine, Tanta University, Egypt.
FAU - Shafik, Noha M
AU  - Shafik NM
AD  - Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Tanta 
      University, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20191115
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (IL1B protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN146 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Acute Disease
MH  - Brain Ischemia/*complications
MH  - Chronic Disease
MH  - DNA Damage/genetics
MH  - Endothelial Cells/*pathology
MH  - Genetic Predisposition to Disease/genetics
MH  - Humans
MH  - Inflammation/genetics/metabolism/pathology
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Oxidative Stress/*genetics
MH  - *Polymorphism, Single Nucleotide
MH  - Schizophrenia/*genetics/metabolism/pathology
MH  - Signal Transduction/genetics
MH  - Stroke/complications/*genetics/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Acute ischemic stroke
OT  - DNA damage
OT  - Polymorphism
OT  - Schizophrenia
OT  - microRNA-146a
EDAT- 2019/11/19 06:00
MHDA- 2020/04/14 06:00
CRDT- 2019/11/19 06:00
PHST- 2019/08/30 00:00 [received]
PHST- 2019/11/09 00:00 [revised]
PHST- 2019/11/12 00:00 [accepted]
PHST- 2019/11/19 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2019/11/19 06:00 [entrez]
AID - S0003-9861(19)30761-1 [pii]
AID - 10.1016/j.abb.2019.108193 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2020 Jan 15;679:108193. doi: 10.1016/j.abb.2019.108193. 
      Epub 2019 Nov 15.