PMID- 31739405
OWN - NLM
STAT- MEDLINE
DCOM- 20200408
LR  - 20200408
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 22
DP  - 2019 Nov 14
TI  - Tricellulin Effect on Paracellular Water Transport.
LID - 10.3390/ijms20225700 [doi]
LID - 5700
AB  - In epithelia, large amounts of water pass by transcellular and paracellular 
      pathways, driven by the osmotic gradient built up by the movement of solutes. The 
      transcellular pathway has been molecularly characterized by the discovery of 
      aquaporin membrane channels. Unlike this, the existence of a paracellular pathway 
      for water through the tight junctions (TJ) was discussed controversially for many 
      years until two molecular components of paracellular water transport, claudin-2 
      and claudin-15, were identified. A main protein of the tricellular TJ (tTJ), 
      tricellulin, was shown to be downregulated in ulcerative colitis leading to 
      increased permeability to macromolecules. Whether or not tricellulin also 
      regulates water transport is unknown yet. To this end, an epithelial cell line 
      featuring properties of a tight epithelium, Madin-Darby canine kidney cells clone 
      7 (MDCK C7), was stably transfected with small hairpin RNA (shRNA) targeting 
      tricellulin, a protein of the tTJ essential for the barrier against passage of 
      solutes up to 10 kDa. Water flux was induced by osmotic gradients using mannitol 
      or 4 and 40 kDa-dextran. Water flux in tricellulin knockdown (KD) cells was 
      higher compared to that of vector controls, indicating a direct role of 
      tricellulin in regulating water permeability in a tight epithelial cell line. We 
      conclude that tricellulin increases water permeability at reduced expression.
FAU - Ayala-Torres, Carlos
AU  - Ayala-Torres C
AD  - Institute of Clinical Physiology/Nutritional Medicine, Medical Department, 
      Division of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, 12203 Berlin, Germany.
FAU - Krug, Susanne M
AU  - Krug SM
AUID- ORCID: 0000-0002-1293-2484
AD  - Institute of Clinical Physiology/Nutritional Medicine, Medical Department, 
      Division of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, 12203 Berlin, Germany.
FAU - Schulzke, Jorg D
AU  - Schulzke JD
AUID- ORCID: 0000-0001-9947-5521
AD  - Institute of Clinical Physiology/Nutritional Medicine, Medical Department, 
      Division of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, 12203 Berlin, Germany.
FAU - Rosenthal, Rita
AU  - Rosenthal R
AD  - Institute of Clinical Physiology/Nutritional Medicine, Medical Department, 
      Division of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, 12203 Berlin, Germany.
FAU - Fromm, Michael
AU  - Fromm M
AUID- ORCID: 0000-0003-4497-7983
AD  - Institute of Clinical Physiology/Nutritional Medicine, Medical Department, 
      Division of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, 12203 Berlin, Germany.
LA  - eng
GR  - GRK 2318/Deutsche Forschungsgemeinschaft/
GR  - (none)/Open Access Publication Fund of the Charite - Universitatsmedizin Berlin/
PT  - Journal Article
DEP - 20191114
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (MARVEL Domain Containing 2 Protein)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Cell Line
MH  - Cell Membrane Permeability
MH  - Dogs
MH  - Epithelium/metabolism
MH  - Gene Knockdown Techniques
MH  - MARVEL Domain Containing 2 Protein/genetics/*metabolism
MH  - Madin Darby Canine Kidney Cells
MH  - Tight Junctions/metabolism
MH  - Water/*metabolism
PMC - PMC6888685
OTO - NOTNLM
OT  - MDCK C7 cells
OT  - paracellular water transport
OT  - tight epithelium
OT  - tricellular tight junction
OT  - tricellulin
COIS- The authors declare no conflict of interest.
EDAT- 2019/11/20 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/11/01
CRDT- 2019/11/20 06:00
PHST- 2019/10/10 00:00 [received]
PHST- 2019/11/07 00:00 [revised]
PHST- 2019/11/12 00:00 [accepted]
PHST- 2019/11/20 06:00 [entrez]
PHST- 2019/11/20 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - ijms20225700 [pii]
AID - ijms-20-05700 [pii]
AID - 10.3390/ijms20225700 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Nov 14;20(22):5700. doi: 10.3390/ijms20225700.